Pattern of acquisition of rotavirus antibody in children followed up from birth to the age of three years

Linhares, Alexandre C.; Melo, Valéria R.; Mascarenhas, Joana D'Arc P.; Gabbay, Yvone B.; Freitas, Ronaldo B. de

doi:10.1590/S0037-86821989000100005

Abstracts

Nine hundred and forty-eight serum samples from 83 children living in Belem, Brazil, collected'within their first three years of life, were testedfor the presence of group- specific rotavirus-antibody by an enzyme-linked immunosorbent assay (ELISA) blocking-test. Passively transferred maternal antibody lasted about two and half months; subsequentely, low levels of rotavirus antibody started to appear at seven months, reaching a peak at eleven months of age. From one year onwards positivity gradually increased, reaching highest values at 34 months of life. Individual responses were examined in sera from 61 children who were followed up since birth to three years of age: 38 (62,3%) ofthem developed a long-term immunity following first infection; eleven (18.0%) children developed a short-term immunity after first infection by rotavirus; seven (11.5%) had no antibody response within their first three years of life; and 5 (8.2%) showed positive antibody response from birth to three years old.

Rotavirus; Antibody; Children

Amostras de soro, em número de 948, foram coletadas de 83 crianças, ao longo de seus três primeiros anos de vida, e testadas quanto àpresença de anticorpos grupo-especificos para rotavirus por um teste de bloqueio, utilizando-se o ensaio imunoenzimático (ELISA). Observou-se que anticorpos transferidos passivamente pela mãe persistiram por cerca de dois e meio meses; subseqüentemente, baixos níveis de anticorpos para rotavirus começaram a aparecer aos sete meses de idade, atingindo um pico por volta dos onze. A partir de um ano apositividade aumentou gradualmente, alcançando os mais altos valores aos 34 meses de vida. Respostas imunes individuais foram avaliadas em 61 crianças acompanhadas desde o seu nascimento até os três anos de idade, obtendo-se os seguintes resultados: 38 (62,3%) delas desenvolveram imunidade de longa duração, após a primo-infecção; 11 (18,0%) indivíduos apresentaram anticorpos específicos que se mantiveram por um intervalo de tempo relativamente curto; 07 (11,5%) não exibiram qualquer resposta imune ao agente em questão, ao longo dos seus três primeiros anos de vida; e 05 (8,2%) mostraram-se positivos quanto à presença de anticorpos para rotavirus, desde o nascimento até os três anos de idade.

Rotavirus; Anticorpos; Crianças

ARTICLES

Pattern of acquisition of rotavirus antibody in children followed up from birth to the age of three years

Alexandre C. Linhares^I; Valéria R. Melo^II; Joana D'Arc P. Mascarenhas^I; Yvone B. Gabbay^I; Ronaldo B. de Freitas^I

^IInstituto Evandro Chagas, Fundação Serviços de Saúde Pública - 66050 - Belém, Pará - Brasil

^IIUniversidade Federal do Pará, Centro de Ciências Biológicas - 66000 - Belém, Pará - Brasil

ABSTRACT

Nine hundred and forty-eight serum samples from 83 children living in Belem, Brazil, collected'within their first three years of life, were testedfor the presence of group- specific rotavirus-antibody by an enzyme-linked immunosorbent assay (ELISA) blocking-test. Passively transferred maternal antibody lasted about two and half months; subsequentely, low levels of rotavirus antibody started to appear at seven months, reaching a peak at eleven months of age. From one year onwards positivity gradually increased, reaching highest values at 34 months of life. Individual responses were examined in sera from 61 children who were followed up since birth to three years of age: 38 (62,3%) ofthem developed a long-term immunity following first infection; eleven (18.0%) children developed a short-term immunity after first infection by rotavirus; seven (11.5%) had no antibody response within their first three years of life; and 5 (8.2%) showed positive antibody response from birth to three years old.

Keywords: Rotavirus. Antibody. Children.

RESUMO

Amostras de soro, em número de 948, foram coletadas de 83 crianças, ao longo de seus três primeiros anos de vida, e testadas quanto àpresença de anticorpos grupo-especificos para rotavirus por um teste de bloqueio, utilizando-se o ensaio imunoenzimático (ELISA). Observou-se que anticorpos transferidos passivamente pela mãe persistiram por cerca de dois e meio meses; subseqüentemente, baixos níveis de anticorpos para rotavirus começaram a aparecer aos sete meses de idade, atingindo um pico por volta dos onze. A partir de um ano apositividade aumentou gradualmente, alcançando os mais altos valores aos 34 meses de vida. Respostas imunes individuais foram avaliadas em 61 crianças acompanhadas desde o seu nascimento até os três anos de idade, obtendo-se os seguintes resultados: 38 (62,3%) delas desenvolveram imunidade de longa duração, após a primo-infecção; 11 (18,0%) indivíduos apresentaram anticorpos específicos que se mantiveram por um intervalo de tempo relativamente curto; 07 (11,5%) não exibiram qualquer resposta imune ao agente em questão, ao longo dos seus três primeiros anos de vida; e 05 (8,2%) mostraram-se positivos quanto à presença de anticorpos para rotavirus, desde o nascimento até os três anos de idade.

Palavras-chave: Rotavirus. Anticorpos. Crianças.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 28/10/88.

This work was supported by the World Health Organization, Control of Diarrhoeal Diseases Programme and Fundação Serviços de Saúde Pública, Ministério da Saúde.

1. Bishop RF. Epidemiology of diarrhoeal disease caused by rotavirus. In: Holmgreen J, Lindberg A, Mollby R (ed.). Development of vaccines and drugs against diarrhoea p. 158-170.11th Nobel Conference, Stockholm, Sweden, 1985.
2. Bishop RF, Barnes GL, Cipriani E, Lund JS. Clinical immunity after neonatal rotavirus infection. A prospective longitudinal study in young childrens. The New England Journal of Medicine 309: 72-76, 1983.
3. Cameron DJ, Bishop RF, Veenstra AA, Barnes GL, Holmes IH, Ruck BJ. Pattern of shedding of two noncultivable viruses in stools of newborn babies. Journal of Medical Virology 2: 7-13, 1978.
4. Candeias JA, Rosenberg CP, Racz ML. Identificação por contraimunoeletroforese de ratavírus em casos de diarréia infantil. Revista de Saúde Pública (São Paulo) 12: 99-103, 1978.
5. Coiro JRR, Bendati MMA, Almeida Neto AJ, Heuser MCF, Vasconcellos VL. Rotavirus infection in Brazilian children with acute enteritis: a seasonal variation study. American Journal of Tropical Medicine Hygiene 32: 1186-1188, 1983.
6. Elias MM. Distribution and titres of rotavirus antibodies in different age groups. Journal of Hygiene 79: 365-372, 1977.
7. GurwithM, Wernnan W, HindeD, Feltham S, Greenberg H. A prospective study of rotavirus infection in infants and young children. Journal of Infectious Diseases 144: 218-224, 1981.
8. Hjelt K, Grauballe PC, Paerregaard A, Nielsen ÒH, KrasilnikoffPA. Protective effect of preexisting rotavirus- specific immunoglobulin A against naturally acquired rotavirus infection in children. Journal of Medical Virology 21: 39-47, 1987.
9. Houly CA, Uchoa MM, Zaidan AM, Gomes-Neto A, de Oliveira FM, Athayde MA, Almeida MFL, Pereira HG. Electrophoretic study of the genome of human rotavirus from Maceio, Brazil. Brazilian Journal of Medical and Biological Research 19: 33-37, 1986.
10. Ishak R, Linhares AC, Gabbay Y, Ishak MOG, Cardoso DDP. Soroepidemiologia de rotavirus em uma população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26: 280-284, 1984.
11. Linhares AC, Ferreira FS, Maues BC, Benchimol JA, Gabbay YB. Prevalência de anticorpos para rotavirus em crianças diarréicas de Belém, Brasil. Revista da Fundação SESP 28: 95-105, 1983.
12. Linhares AC, Gabbay YB, Freitas RB, Mascarenhas JDP, Flewett TH, Beards GM. Epidemiology of rotavirus subgroups and serotypes in Belem, Brazil: a three- year study. Annales de l'Institut Pasteur/ Virologie 139: 89-99, 1988.
13. Linhares AC, Gabbay YB, Freitas RB, Mascarenhas JDP, Salbe E. Longitudinal study of rotavirus infections among children living in Belem, Brazil. Epidemiology and Infection. In press.
14. Linhares AC, Monção HC, Gabbay YB, de Araujo VL, Serruya AC, Loureiro EC. Acute diarrhoea associated with rotavirus among children living in Belem, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 77: 384-390, 1983.
15. Linhares AC, Pinheiro FP, Schmetz C, Muller G, Peters D. Douvirus (rotavirus) em Belém do Pará, Brasil, Nota Prévia. Revista do Instituto de Medicina Tropical de São Paulo 19: 278-279, 1977.
16. Linhares AC, Salbé EV, Gabbay YB, Rees N. Prevalence of rotavirus antibody among isolated South American Indian communities. American Journal of Epidemiology 123: 699-709, 1986.
17. MataL, Simhon A, Urrutia JJ, KronmalRA, Femández R, Garcia B. Epidemiology of rotaviruses in a cohort of 45 Guatamalan Mayan Indian children observed from birth to the age of three years. Journal of Infectious Diseases 148: 452-461, 1983.
18. Parrino TA, Schreiber DS, Trier JS, Kapikian AZ, Blacklow N. Clinical immunity in acute gastroenteritis caused by Norwalk agent The New England Journal of Medicine 297: 86-89, 1977.
19. Pereira HG, Azeredo RS, Leite JP, Candeias JA, Racz ML, Linhares AC, Gabbay YB, Trabulsi JR. Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Para, Brazil, Journal of Hygiene 90: 117-125, 1983.
20. Riepenhoff-Talty M, Bogger-Goren S, Li P, Carmody PJ, Barret HJ, Ogra PL. Development of serum and intestinal antibody response to rotavirus after naturally acquired rotavirus infection in man. Journal of Medical Virology 8: 215-222, 1981.
21. Rowland MG, Goh SG, Williams K, Campbell AD, Beards GM, Sanders RC, Flewett TH. Epidemiological aspects of rotavirus infection in young Gambian children. Annals of Tropical Paediatrics 5: 23-28, 1985.
22. Totterdell BM, Nicholson KG, MacLeod J, Chrystie IL, Banatvala JE. Neonatal rotavirus infection: role of lacteal neutralising alpha anti-trypsin and nonimmunoglobulin antiviral activity in protection. Journal of Medical Virology 10: 37-44, 1982.

Publication Dates

Publication in this collection
27 May 2013
Date of issue
Mar 1989

History

Accepted
28 Oct 1988
Received
28 Oct 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Bishop RF. Epidemiology of diarrhoeal disease caused by rotavirus. In: Holmgreen J, Lindberg A, Mollby R (ed.). Development of vaccines and drugs against diarrhoea p. 158-170.11th Nobel Conference, Stockholm, Sweden, 1985.

[2] 2. Bishop RF, Barnes GL, Cipriani E, Lund JS. Clinical immunity after neonatal rotavirus infection. A prospective longitudinal study in young childrens. The New England Journal of Medicine 309: 72-76, 1983.

[3] 3. Cameron DJ, Bishop RF, Veenstra AA, Barnes GL, Holmes IH, Ruck BJ. Pattern of shedding of two noncultivable viruses in stools of newborn babies. Journal of Medical Virology 2: 7-13, 1978.

[4] 4. Candeias JA, Rosenberg CP, Racz ML. Identificação por contraimunoeletroforese de ratavírus em casos de diarréia infantil. Revista de Saúde Pública (São Paulo) 12: 99-103, 1978.

[5] 5. Coiro JRR, Bendati MMA, Almeida Neto AJ, Heuser MCF, Vasconcellos VL. Rotavirus infection in Brazilian children with acute enteritis: a seasonal variation study. American Journal of Tropical Medicine Hygiene 32: 1186-1188, 1983.

[6] 6. Elias MM. Distribution and titres of rotavirus antibodies in different age groups. Journal of Hygiene 79: 365-372, 1977.

[7] 7. GurwithM, Wernnan W, HindeD, Feltham S, Greenberg H. A prospective study of rotavirus infection in infants and young children. Journal of Infectious Diseases 144: 218-224, 1981.

[8] 8. Hjelt K, Grauballe PC, Paerregaard A, Nielsen ÒH, KrasilnikoffPA. Protective effect of preexisting rotavirus- specific immunoglobulin A against naturally acquired rotavirus infection in children. Journal of Medical Virology 21: 39-47, 1987.

[9] 9. Houly CA, Uchoa MM, Zaidan AM, Gomes-Neto A, de Oliveira FM, Athayde MA, Almeida MFL, Pereira HG. Electrophoretic study of the genome of human rotavirus from Maceio, Brazil. Brazilian Journal of Medical and Biological Research 19: 33-37, 1986.

[10] 10. Ishak R, Linhares AC, Gabbay Y, Ishak MOG, Cardoso DDP. Soroepidemiologia de rotavirus em uma população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26: 280-284, 1984.

[11] 11. Linhares AC, Ferreira FS, Maues BC, Benchimol JA, Gabbay YB. Prevalência de anticorpos para rotavirus em crianças diarréicas de Belém, Brasil. Revista da Fundação SESP 28: 95-105, 1983.

[12] 12. Linhares AC, Gabbay YB, Freitas RB, Mascarenhas JDP, Flewett TH, Beards GM. Epidemiology of rotavirus subgroups and serotypes in Belem, Brazil: a three- year study. Annales de l'Institut Pasteur/ Virologie 139: 89-99, 1988.

[13] 13. Linhares AC, Gabbay YB, Freitas RB, Mascarenhas JDP, Salbe E. Longitudinal study of rotavirus infections among children living in Belem, Brazil. Epidemiology and Infection. In press.

[14] 14. Linhares AC, Monção HC, Gabbay YB, de Araujo VL, Serruya AC, Loureiro EC. Acute diarrhoea associated with rotavirus among children living in Belem, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 77: 384-390, 1983.

[15] 15. Linhares AC, Pinheiro FP, Schmetz C, Muller G, Peters D. Douvirus (rotavirus) em Belém do Pará, Brasil, Nota Prévia. Revista do Instituto de Medicina Tropical de São Paulo 19: 278-279, 1977.

[16] 16. Linhares AC, Salbé EV, Gabbay YB, Rees N. Prevalence of rotavirus antibody among isolated South American Indian communities. American Journal of Epidemiology 123: 699-709, 1986.

[17] 17. MataL, Simhon A, Urrutia JJ, KronmalRA, Femández R, Garcia B. Epidemiology of rotaviruses in a cohort of 45 Guatamalan Mayan Indian children observed from birth to the age of three years. Journal of Infectious Diseases 148: 452-461, 1983.

[18] 18. Parrino TA, Schreiber DS, Trier JS, Kapikian AZ, Blacklow N. Clinical immunity in acute gastroenteritis caused by Norwalk agent The New England Journal of Medicine 297: 86-89, 1977.

[19] 19. Pereira HG, Azeredo RS, Leite JP, Candeias JA, Racz ML, Linhares AC, Gabbay YB, Trabulsi JR. Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Para, Brazil, Journal of Hygiene 90: 117-125, 1983.

[20] 20. Riepenhoff-Talty M, Bogger-Goren S, Li P, Carmody PJ, Barret HJ, Ogra PL. Development of serum and intestinal antibody response to rotavirus after naturally acquired rotavirus infection in man. Journal of Medical Virology 8: 215-222, 1981.

[21] 21. Rowland MG, Goh SG, Williams K, Campbell AD, Beards GM, Sanders RC, Flewett TH. Epidemiological aspects of rotavirus infection in young Gambian children. Annals of Tropical Paediatrics 5: 23-28, 1985.

[22] 22. Totterdell BM, Nicholson KG, MacLeod J, Chrystie IL, Banatvala JE. Neonatal rotavirus infection: role of lacteal neutralising alpha anti-trypsin and nonimmunoglobulin antiviral activity in protection. Journal of Medical Virology 10: 37-44, 1982.