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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682

Rev. Soc. Bras. Med. Trop. vol.22 no.2 Uberaba Apr./June 1989

http://dx.doi.org/10.1590/S0037-86821989000200001 

ARTIGOS

 

Ocorrência de rotavírus e adenovírus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia

 

 

Divina das Dores P. CardosoI; Wilia Marta E.D. de BritoI; Regina Maria Bringel MartinsI; Eliott W. KitajimaII; Marieta P. M. SouzaI; Aristides José BarbosaI; Solimar A. de OliveiraI; Silvana B. RascopiI

ILaboratório de Virologia do Instituto de Patologia Tropical e Saúde Pública. Universidade Federal de Goiás - Goiânia, GO
IIDepartamento de Biologia Celular - Fundação Universidade de Brasília - Brasília, DF. Suporte Financeiro da FINEP

Endereço para correspondência

 

 


RESUMO

Analisaram-se 300 amostras fecais de crianças na faixa etária de até 8 anos, na cidade de Goiânia, objetivando-se determinar o percentual de rotavirus e adenovirus em processos diarréicos, frente a outros microorganismos patogênicos entéricos (bactérias ou parasitos). Rotavirus foram detectados isoladamente em 47 casos e, associando-se a outros agentes, em 21 casos. Adenovirus foram encontrados em 7 casos, sendo que em 6 ocorreram isoladamente e em 1, associaram-se a outro microrganismo. Utilizaram-se três metodologias para a análise virológica: o ensaio imunoenzimático adaptado para rotavirus e adenovirus (EIARA), a eletroforese em gel de poliacrilamida (EGPA) e a imunomicroscopia eletrônica (IME). O índice de concordância entre as três metodologias foi de 92,8%, entre EGPA e EIARA de 95,8% e entre a IME e ElARA de 100,0%. Dentre as amostras de rotavirus, 97,0% mostraram perfil eletroforético longo, compatível com o subgrupo II.

Palavras-chave: Adenovirus. Rotavirus. Gastrenterite.


ABSTRACT

In an attempt to detect rotavirus and adenovirus prevalence among other enteropathogens (bacteria and parasites) in diarrhoea, three hundred fecal samples originating from children living in Goiânia city (Goiás state, Brazil) were analysed. Rotavirus was found to be the only pathogen in 47 cases, and associated with other infectious agents in 21 cases. 97,0% positive samples of rotavirus showed an electrophoretic pattern characteristic of subgroup II. Adenovirus was found in 7 cases, and associated with other microrganisms in 1 case. Three methods were applied for virological analyses: enzyme immunoassay for rotavirus and adenovirus (EIARA), polyacrylamide gel electrophoresis (PAGE) and immunoelectron microscopy (IEM). The concordance among the three methods was 92.8%, PAGE and EIARA agreed in 95.8%, and IEM and EIARA agreed in 100.0%.

Keywords: Adenovirus. Rotavirus. Gastroenteritis.


 

 

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Full text available only in PDF format.

 

 

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Endereço para correspondência:
Profa. Divina das Dores P. Cardoso.
Depto. de Microbiologia/IPTSP/UFG.
Pça. Universitária s/n.
74000
Goiânia, GO.

 

 

Recebido para publicação em 15/12/88

 

 

Suporte Financeiro da FINEP.

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