Ocorrência de rotavírus e adenovírus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia

Cardoso, Divina das Dores P.; Brito, Wilia Marta E.D. de; Martins, Regina Maria Bringel; Kitajima, Eliott W.; Souza, Marieta P. M.; Barbosa, Aristides José; Oliveira, Solimar A. de; Rascopi, Silvana B.

doi:10.1590/S0037-86821989000200001

Resumos

Analisaram-se 300 amostras fecais de crianças na faixa etária de até 8 anos, na cidade de Goiânia, objetivando-se determinar o percentual de rotavirus e adenovirus em processos diarréicos, frente a outros microorganismos patogênicos entéricos (bactérias ou parasitos). Rotavirus foram detectados isoladamente em 47 casos e, associando-se a outros agentes, em 21 casos. Adenovirus foram encontrados em 7 casos, sendo que em 6 ocorreram isoladamente e em 1, associaram-se a outro microrganismo. Utilizaram-se três metodologias para a análise virológica: o ensaio imunoenzimático adaptado para rotavirus e adenovirus (EIARA), a eletroforese em gel de poliacrilamida (EGPA) e a imunomicroscopia eletrônica (IME). O índice de concordância entre as três metodologias foi de 92,8%, entre EGPA e EIARA de 95,8% e entre a IME e ElARA de 100,0%. Dentre as amostras de rotavirus, 97,0% mostraram perfil eletroforético longo, compatível com o subgrupo II.

Adenovirus; Rotavirus; Gastrenterite

In an attempt to detect rotavirus and adenovirus prevalence among other enteropathogens (bacteria and parasites) in diarrhoea, three hundred fecal samples originating from children living in Goiânia city (Goiás state, Brazil) were analysed. Rotavirus was found to be the only pathogen in 47 cases, and associated with other infectious agents in 21 cases. 97,0% positive samples of rotavirus showed an electrophoretic pattern characteristic of subgroup II. Adenovirus was found in 7 cases, and associated with other microrganisms in 1 case. Three methods were applied for virological analyses: enzyme immunoassay for rotavirus and adenovirus (EIARA), polyacrylamide gel electrophoresis (PAGE) and immunoelectron microscopy (IEM). The concordance among the three methods was 92.8%, PAGE and EIARA agreed in 95.8%, and IEM and EIARA agreed in 100.0%.

Adenovirus; Rotavirus; Gastroenteritis

ARTIGOS

Ocorrência de rotavírus e adenovírus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia

Divina das Dores P. Cardoso^I; Wilia Marta E.D. de Brito^I; Regina Maria Bringel Martins^I; Eliott W. Kitajima^II; Marieta P. M. Souza^I; Aristides José Barbosa^I; Solimar A. de Oliveira^I; Silvana B. Rascopi^I

^ILaboratório de Virologia do Instituto de Patologia Tropical e Saúde Pública. Universidade Federal de Goiás - Goiânia, GO

^IIDepartamento de Biologia Celular - Fundação Universidade de Brasília - Brasília, DF. Suporte Financeiro da FINEP

^{Endereço para correspondência} Endereço para correspondência: Profa. Divina das Dores P. Cardoso. Depto. de Microbiologia/IPTSP/UFG. Pça. Universitária s/n. 74000 Goiânia, GO.

RESUMO

Analisaram-se 300 amostras fecais de crianças na faixa etária de até 8 anos, na cidade de Goiânia, objetivando-se determinar o percentual de rotavirus e adenovirus em processos diarréicos, frente a outros microorganismos patogênicos entéricos (bactérias ou parasitos). Rotavirus foram detectados isoladamente em 47 casos e, associando-se a outros agentes, em 21 casos. Adenovirus foram encontrados em 7 casos, sendo que em 6 ocorreram isoladamente e em 1, associaram-se a outro microrganismo. Utilizaram-se três metodologias para a análise virológica: o ensaio imunoenzimático adaptado para rotavirus e adenovirus (EIARA), a eletroforese em gel de poliacrilamida (EGPA) e a imunomicroscopia eletrônica (IME). O índice de concordância entre as três metodologias foi de 92,8%, entre EGPA e EIARA de 95,8% e entre a IME e ElARA de 100,0%. Dentre as amostras de rotavirus, 97,0% mostraram perfil eletroforético longo, compatível com o subgrupo II.

Palavras-chave: Adenovirus. Rotavirus. Gastrenterite.

ABSTRACT

In an attempt to detect rotavirus and adenovirus prevalence among other enteropathogens (bacteria and parasites) in diarrhoea, three hundred fecal samples originating from children living in Goiânia city (Goiás state, Brazil) were analysed. Rotavirus was found to be the only pathogen in 47 cases, and associated with other infectious agents in 21 cases. 97,0% positive samples of rotavirus showed an electrophoretic pattern characteristic of subgroup II. Adenovirus was found in 7 cases, and associated with other microrganisms in 1 case. Three methods were applied for virological analyses: enzyme immunoassay for rotavirus and adenovirus (EIARA), polyacrylamide gel electrophoresis (PAGE) and immunoelectron microscopy (IEM). The concordance among the three methods was 92.8%, PAGE and EIARA agreed in 95.8%, and IEM and EIARA agreed in 100.0%.

Keywords: Adenovirus. Rotavirus. Gastroenteritis.

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Recebido para publicação em 15/12/88

Suporte Financeiro da FINEP.

1. Azeredo RS, Leite JPG, Pereira HG, Vidal MNP, Andrade ZP, Farias V, Sutmoller F, Schatzmayr HG. Agentes virais associados à gastroenterite infantil no Rio de Janeiro - 1^o semestre 1983. Boletim Epidemiológico do Ministério da Saúde XV: 193-194, 1983.
2. Barth, OM. Estudo sobre a contrastação negativa de suspensões virais. Revista Brasileira de Biologia 44:71- 80, 1984.
3. Chiba S, Nakata S, Nakamura I, Tamiguchi K, Urasawa S, Fujinaga K, Nakao T. Outbreak of infantile gastroenteritis due to type 40 adenovirus. Lancet II: 954-957, 1983.
4. Clarke IN, McCrae MA. Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity 36:492-497,1982.
5. Cukor G, Blacklow NR. Human viral gastroenteritis. Microbiological Reviews 48:157-179, 1984.
6. De Jong JC, Wigand R, Kidd AH, Wadell G, Kapsenberg JG, Muzerie CJ, Wermenbol AG, Firtzlaff RG. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. Journal of Medical Virology 11:215-231, 1983.
7. Dimitrov DH, Graham DY, Lopez J, Muchinik G, Velasco G, Stenback WA, Estes MK. RNA electropherotypes of human rotaviruses from North and South America. Bulletin of the World Health Organization 62:321-329, 1984.
8. Edwards PR, Ewing WH. Identification of Enterobacteriaceae (3^rd edit) Minneapolis, Minnesota: Burgess Publishing Company, 1972.
9. Evans Jr DJ, Evans DG. Direct serological assay for the heat labile enterotoxin of Escherichia coli, using passive immune hemolysis. Infection and Immunity 16:604-609, 1977.
10. Faust EC, D'Antoni JS, Odan V, Miller MJ, Peres C Sawitz W, Thomen LF, Tobie J, Walker JM. Critical study of clinical laboratory techniques for diagnosis of protozoan cysts and helminth eggs in feces. Preliminary communication. American Journal of Tropical Medicine 18:169-183, 1938.
11. Flewett TH, Bryden AS, Davies H. Epidemic viral ententes in a long-stay children's ward. Lancet 1:4-5, 1975.
12. Gary Jr GW, Hierholzer JC, Black RE. Characteristics of noncultivable adenoviruses associated with diarrhea in infants: a new subgroup of human adenoviruses. Journal of Clinical Microbiology 10:96-103, 1979.
13. Gómez JA, Biscotti EI, Bercovich JA, Grinstein S. Epidemiology of human rotaviruses in Argentina as determined by RNA genome electrophoresis. Intervirology 26:174-180, 1986.
14. Hoffman WA, Pons JA, Janer JL. Sedimentation-concentration method in Schistosomiasis mansoni Puerto Rico Journal of Public Health and Tropical Medicine 9:283-291, 1934.
15. Holland J, Spindler K, Horodyski F, Graban E, Nichol S, Vandepol S. Rapid evolution of RNA genomes. Science 215:1577-1585, 1982.
16. Hundley F, Biryahwaho B, Gow M, Desseeberger V. Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. Virology 143:88-103, 1985.
17. IshakR, Linhares AC, Gabbay Y,IshakMDG, Cardoso DDP. Soroepidemiologia de rotavirus em uma população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26:280-284, 1984.
18. Johansson ME, Uhnoo I, Kidd AH, Madeley CR, Wadell G. Direct identification of enteric adenovirus a candidate new serotype associated with infantile gastroenteritis. Journal of Clinical Microbiology 12:95-100, 1980.
19. Kapikian AZ, Cline WL, Kim HW, Kalika AR, Wyatl RG, Kirkvan DH. Chanock RM, James Jr HD, Vaughn AL. Antigenic relationships among five reovirus like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proceedings of the Society for Experimental Biology and Medicine 152:535-539, 1976.
20. Leite JP, Pereira HG, Azeredo RS, Schatzmayr HG. Adenoviruses in faeces of children with acute gastroenteritis in Rio de Janeiro, Brazil. Journal of Medical Virology 15:203-209, 1985.
21. Linhares AC, Ferreira FS, Mavues BC, Benchimol JA, Gabbay YB. Prevalência de anticorpos para rotavirus em crianças diarrèicas de Belém, Brasil. Revista da Fundação SE SP 28:95-105, 1983.
22. Linhares AC, Gabbay YB, Freitas RB, Silva IX, Vieira JJA, Ferreira SF, Figueiras ACM, Loureiro MGS. Estudo longitudinal das infecções por rotavirus em crianças de Belém, Brasil: Primeiros dezoito meses. Boletim Epidemiológico do Ministério da Saúde XVII: 49-59, 1985.
23. Linhares AC, Monção HC, Gabbay YB, Araujo VLC, Serruya AC, Loureiro ECB. Acute diarrhoea associated with rotavirus among children living in Belém, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 77:384-390, 1983.
24. Linhares AC, Pinheiro FP, Freitas RB, Gabbay YB, Shirley JA, Beards GM. An outbreak of rotavirus diarrhea among a nonimmune isolated South American Indian community. American Journal of Epidemiology 113:703-710, 1981.
25. Middleton RJ, Szymanski HI, Petrie M. Viruses associated with acute gastroenteritis in young children. American Journal of Diseases of Children 131:733-737, 1987.
26. Pedley S, Bridger JC. Brown JF, McCrae MA. Molecular characterization of rotaviruses with distinct group antigens. Journal of General Virology 64:2093-2101, 1983.
27. Pereira HG, Azeredo RS, Leite JP, Andrade ZP, Castro L. A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). Journal of Virological Methods 10:21-28, 1985.
28. Pereira HG, Azeredo RS, Leite JPG, Barth OM, Sutmoller F, Farias V, Vidal MNP. Comparison of polyacrylamide gel electrophoresis (PAGE), immunoelectron microscopy (IEM) and enzyme immunoassay (EIA) for the rapid diagnoses of rotavirus infection in children. Memórias do Instituto Oswaldo Cruz 78:483- 490, 1983.
29. Pereira HG, Azeredo RS, Leite JP, Candeias JA, Rácz ML, Linhares AC, Gabbay YB, Trabulsi JR. Electrophoretic study of the genome of human rotaviruses from Rio de J aneiro, São Paulo and Pará, Brazil. Journal of Hygiene 90:117-125, 1983.
30. Retter M, Middleton PJ, Tam JS, Petrie M. Enteric adenoviruses: detection, replication, and significance. Journal of Clinical Microbiology 10:574-578, 1979.
31. Richmond SJ, Caul EO, Dunn SM, Ashley CR, Clarke SKR. An outbreak of gastroenteritis in young children caused by adenoviruses. The Lancet1:1178-1181,1979.
32. Schnags RD, Rodger SM, Holmes IH. Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in Central Australia. Infection and Immunity 33:17-21, 1981.
33. Uhnool, Wadell G., Svensson L, Johansson M. Two new serotypes of enteric adenovirus causing infantile diarrhoea. Developments in Biological Standardization 53:311-318, 1983.
34. Whitelaw A, Davies H, Parry J. Electron microscopy of fatal adenovirus gastroenteritis. The Lancet 1:361,1977.
35. World Health Organization, scientific activities. Bulletin of the World Health Organization 63:233-235, 1985.

Endereço para correspondência:

Profa. Divina das Dores P. Cardoso.

Depto. de Microbiologia/IPTSP/UFG.

Pça. Universitária s/n.

74000

Goiânia, GO.

Datas de Publicação

Publicação nesta coleção
27 Maio 2013
Data do Fascículo
Jun 1989

Histórico

Recebido
15 Dez 1988
Aceito
15 Dez 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Azeredo RS, Leite JPG, Pereira HG, Vidal MNP, Andrade ZP, Farias V, Sutmoller F, Schatzmayr HG. Agentes virais associados à gastroenterite infantil no Rio de Janeiro - 1^o semestre 1983. Boletim Epidemiológico do Ministério da Saúde XV: 193-194, 1983.

[2] 2. Barth, OM. Estudo sobre a contrastação negativa de suspensões virais. Revista Brasileira de Biologia 44:71- 80, 1984.

[3] 3. Chiba S, Nakata S, Nakamura I, Tamiguchi K, Urasawa S, Fujinaga K, Nakao T. Outbreak of infantile gastroenteritis due to type 40 adenovirus. Lancet II: 954-957, 1983.

[4] 4. Clarke IN, McCrae MA. Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity 36:492-497,1982.

[5] 5. Cukor G, Blacklow NR. Human viral gastroenteritis. Microbiological Reviews 48:157-179, 1984.

[6] 6. De Jong JC, Wigand R, Kidd AH, Wadell G, Kapsenberg JG, Muzerie CJ, Wermenbol AG, Firtzlaff RG. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. Journal of Medical Virology 11:215-231, 1983.

[7] 7. Dimitrov DH, Graham DY, Lopez J, Muchinik G, Velasco G, Stenback WA, Estes MK. RNA electropherotypes of human rotaviruses from North and South America. Bulletin of the World Health Organization 62:321-329, 1984.

[8] 8. Edwards PR, Ewing WH. Identification of Enterobacteriaceae (3^rd edit) Minneapolis, Minnesota: Burgess Publishing Company, 1972.

[9] 9. Evans Jr DJ, Evans DG. Direct serological assay for the heat labile enterotoxin of Escherichia coli, using passive immune hemolysis. Infection and Immunity 16:604-609, 1977.

[10] 10. Faust EC, D'Antoni JS, Odan V, Miller MJ, Peres C Sawitz W, Thomen LF, Tobie J, Walker JM. Critical study of clinical laboratory techniques for diagnosis of protozoan cysts and helminth eggs in feces. Preliminary communication. American Journal of Tropical Medicine 18:169-183, 1938.

[11] 11. Flewett TH, Bryden AS, Davies H. Epidemic viral ententes in a long-stay children's ward. Lancet 1:4-5, 1975.

[12] 12. Gary Jr GW, Hierholzer JC, Black RE. Characteristics of noncultivable adenoviruses associated with diarrhea in infants: a new subgroup of human adenoviruses. Journal of Clinical Microbiology 10:96-103, 1979.

[13] 13. Gómez JA, Biscotti EI, Bercovich JA, Grinstein S. Epidemiology of human rotaviruses in Argentina as determined by RNA genome electrophoresis. Intervirology 26:174-180, 1986.

[14] 14. Hoffman WA, Pons JA, Janer JL. Sedimentation-concentration method in Schistosomiasis mansoni Puerto Rico Journal of Public Health and Tropical Medicine 9:283-291, 1934.

[15] 15. Holland J, Spindler K, Horodyski F, Graban E, Nichol S, Vandepol S. Rapid evolution of RNA genomes. Science 215:1577-1585, 1982.

[16] 16. Hundley F, Biryahwaho B, Gow M, Desseeberger V. Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. Virology 143:88-103, 1985.

[17] 17. IshakR, Linhares AC, Gabbay Y,IshakMDG, Cardoso DDP. Soroepidemiologia de rotavirus em uma população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26:280-284, 1984.

[18] 18. Johansson ME, Uhnoo I, Kidd AH, Madeley CR, Wadell G. Direct identification of enteric adenovirus a candidate new serotype associated with infantile gastroenteritis. Journal of Clinical Microbiology 12:95-100, 1980.

[19] 19. Kapikian AZ, Cline WL, Kim HW, Kalika AR, Wyatl RG, Kirkvan DH. Chanock RM, James Jr HD, Vaughn AL. Antigenic relationships among five reovirus like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proceedings of the Society for Experimental Biology and Medicine 152:535-539, 1976.

[20] 20. Leite JP, Pereira HG, Azeredo RS, Schatzmayr HG. Adenoviruses in faeces of children with acute gastroenteritis in Rio de Janeiro, Brazil. Journal of Medical Virology 15:203-209, 1985.

[21] 21. Linhares AC, Ferreira FS, Mavues BC, Benchimol JA, Gabbay YB. Prevalência de anticorpos para rotavirus em crianças diarrèicas de Belém, Brasil. Revista da Fundação SE SP 28:95-105, 1983.

[22] 22. Linhares AC, Gabbay YB, Freitas RB, Silva IX, Vieira JJA, Ferreira SF, Figueiras ACM, Loureiro MGS. Estudo longitudinal das infecções por rotavirus em crianças de Belém, Brasil: Primeiros dezoito meses. Boletim Epidemiológico do Ministério da Saúde XVII: 49-59, 1985.

[23] 23. Linhares AC, Monção HC, Gabbay YB, Araujo VLC, Serruya AC, Loureiro ECB. Acute diarrhoea associated with rotavirus among children living in Belém, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 77:384-390, 1983.

[24] 24. Linhares AC, Pinheiro FP, Freitas RB, Gabbay YB, Shirley JA, Beards GM. An outbreak of rotavirus diarrhea among a nonimmune isolated South American Indian community. American Journal of Epidemiology 113:703-710, 1981.

[25] 25. Middleton RJ, Szymanski HI, Petrie M. Viruses associated with acute gastroenteritis in young children. American Journal of Diseases of Children 131:733-737, 1987.

[26] 26. Pedley S, Bridger JC. Brown JF, McCrae MA. Molecular characterization of rotaviruses with distinct group antigens. Journal of General Virology 64:2093-2101, 1983.

[27] 27. Pereira HG, Azeredo RS, Leite JP, Andrade ZP, Castro L. A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). Journal of Virological Methods 10:21-28, 1985.

[28] 28. Pereira HG, Azeredo RS, Leite JPG, Barth OM, Sutmoller F, Farias V, Vidal MNP. Comparison of polyacrylamide gel electrophoresis (PAGE), immunoelectron microscopy (IEM) and enzyme immunoassay (EIA) for the rapid diagnoses of rotavirus infection in children. Memórias do Instituto Oswaldo Cruz 78:483- 490, 1983.

[29] 29. Pereira HG, Azeredo RS, Leite JP, Candeias JA, Rácz ML, Linhares AC, Gabbay YB, Trabulsi JR. Electrophoretic study of the genome of human rotaviruses from Rio de J aneiro, São Paulo and Pará, Brazil. Journal of Hygiene 90:117-125, 1983.

[30] 30. Retter M, Middleton PJ, Tam JS, Petrie M. Enteric adenoviruses: detection, replication, and significance. Journal of Clinical Microbiology 10:574-578, 1979.

[31] 31. Richmond SJ, Caul EO, Dunn SM, Ashley CR, Clarke SKR. An outbreak of gastroenteritis in young children caused by adenoviruses. The Lancet1:1178-1181,1979.

[32] 32. Schnags RD, Rodger SM, Holmes IH. Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in Central Australia. Infection and Immunity 33:17-21, 1981.

[33] 33. Uhnool, Wadell G., Svensson L, Johansson M. Two new serotypes of enteric adenovirus causing infantile diarrhoea. Developments in Biological Standardization 53:311-318, 1983.

[34] 34. Whitelaw A, Davies H, Parry J. Electron microscopy of fatal adenovirus gastroenteritis. The Lancet 1:361,1977.

[35] 35. World Health Organization, scientific activities. Bulletin of the World Health Organization 63:233-235, 1985.

Brasil

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Ocorrência de rotavírus e adenovírus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia

Resumos

Endereço para correspondência:

Datas de Publicação

Histórico