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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682

Rev. Soc. Bras. Med. Trop. vol.23 no.1 Uberaba Jan./Mar. 1990

https://doi.org/10.1590/S0037-86821990000100003 

ARTIGOS

 

Uso de células de Aedes albopictus C6/36 na propagação e classificação de arbovírus das famílias Togaviridae, Flaviviridae, Bunyaviridae e Rhabdoviridae

 

 

Luiz Tadeu Moraes Figueiredo

Endereço para correspondência

 

 


RESUMO

Colônias de células de mosquito Aedes albopictus C6/36 foram infectadas com 23 arbovirus, sendo 19 destes existentes no Brasil, pertencentes às famílias Togaviridae, Flaviviridae, Bunyaviridae e Rhabdoviridae. A Replicação virai foi detectada por imunofluorescência indireta com todos os vírus estudados enquanto que o efeito citopático foi observado durante a infecção por alguns destes. No teste de imunofluorescência indireta utilizou-se fluidos ascíticos imunes de camundongos, específicos para os vírus estudados. A replicação virai caracterizada por grande produção de antígeno recomenda a utilização de células C6/36 na propagação e em tentativas de isolamento desses arbovirus. A técnica de imunofluorescência ofereceu importantes subsídios na classificação e identificação de vírus que replicam nestas células.

Palavras-chave: Arbovirus. Culturas de células de mosquito. Imunofluorescência Indireta. Isolamento de vírus.


ABSTRACT

C6/36 Aedes albopictus cells were infected w ith Brazilian arbovirus from the families Togaviridae, Flaviviridae, Bunyaviridae and Rhabdoviridae. Replication was obtained with all the studied viruses and cytopathic effect was observed with some. Viral antigen was assayed in C6/36 cell cultures for antigen was assayed in C6/36 cells by an indirect immunofluorescence test using specific mouse immune ascitic fluid. Antigen production was detected in C6/36 cells infected with all the studied viruses. The author recommends the inoculation of C6/36 cell cultures for isolation of virus from the four studied families. The immunofluorescence technique is an important tool for classification and identification of virus growing in C6/36 cells.

Keywords: Arbovirus. Mosquito tissue culture cells. Indirect imunofluorescence. Virus isolation.


 

 

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Endereço para correspondência:
Dr. Luiz Tadeu Moraes Figueiredo.
Departamento de Clínica Médica.
Facuidade de Medicina de Ribeirão Preto/USP.
14049
Ribeirão Preto, SP, Brasil.

 

 

Recebido para publicação em 03/08/89.

 

 

Trabalho efetuado no Departamento de Virologia do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. Financiado em parte pelo CNPq (302509/87-9/BM/FV) e pela SUCAM.

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