Sporothrix brasiliensis produces the highest levels of oxidative stress in a murine model among the species of the Sporothrix schenckii complex

Abstract

INTRODUCTION:

We compared indicators of oxidative stress in the tissue of mice infected with strains from Sporothrix schenckii complex.

METHODS:

Mice were inoculated with Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, Sporothrix mexicana or Sporothrix albicans. The activity of catalase and glutathione were accessed in the liver and spleen.

RESULTS:

Animals infected with S. brasiliensis exhibited splenomegaly and significant decrease in catalase activity, and protein and non-protein thiol content compared to animals infected with the other species.

CONCLUSIONS:

Sporothrix brasiliensis exhibits higher pathogenicity compared to other species of the Sporothrix schenckii complex by increasing oxidative stress in animal tissue.

Key words:
Sporotrichosis; Antioxidant enzymes; Oxidative stress

The genus Sporothrix was recently reclassified as a complex including at least six species capable of causing sporotrichosis¹. Subsequently, studies have been conducted in order to elucidate the different levels of virulence and pathogenicity demonstrated by each species². According to previous studies, S. brasiliensis demonstrated the highest pathogenicity and virulence, while isolates of S. schenckii sensu stricto (s. str.) showed marked heterogeneity in the virulence profiles studied^2,3. Sporotrichosis has a worldwide distribution and is the most common subcutaneous fungal infection in South America⁴. It is mainly acquired by traumatic inoculation of the subcutaneous tissue by the organism and can be disseminated to other parts of the body, particularly in immunocompromised patients⁴.

The ability of the fungal pathogen to cause disease depends on its survival in the host via evasion of the immune system, especially the antimicrobial mechanism of phagocytes^5,6. Immune cell function is closely linked to the generation of reactive oxygen species (ROS) and is strongly influenced by the redox potential of the extracellular environment. Furthermore, the intracellular oxidant/antioxidant balance is an important determinant of the immune cell’s activity; the antioxidant level in immune cells plays a crucial role in protection against oxidative stress and preserving adequate function. Sporotrichosis causes tissue damage, which can lead to a state of oxidative stress, thereby reducing the immune response and enhancing pathogen survival in the host⁶.

It has been recognized that similar characteristics expressed by strains of S. brasiliensis and S. schenckii, but not by other species of the complex, result in these two species being the most pathogenic of the group. In the present study, we investigated differences in response to the oxidative stress induced by experimental infection with five species of the Sporothrix schenckii complex in a mouse model.

One strain each of the following species was included in the study: S. brasiliensis CBS 1330, S. schenckii s. str. Ss 02, S. globosa CBS 132922, S. mexicana CBS 120341, and S. albicans PG 03. The fungi were stored in slant cultures covered with sterile paraffin oil and subcultured on Sabouraud dextrose agar (SDA) plates at 30°C for 7 days.

Four-week-old BALB/c male mice (Animal House, Federal University of Santa Maria, Brazil) with a mean weight of 30g were used. The mice were housed in standard boxes with adequate access to food and water in a room with controlled temperature (22 ± 2°C) and subjected to a 12h light-dark cycle with lights on at 7:00am. The entire experiment was performed in accordance with the guidelines of the National Council for the Control of Animal Experimentation (CONCEA).

A slice of the plate culture was inoculated into Sabouraud dextrose broth. The mycelia of the strains were grown at 25°C for 7 days using a rotary shaker at 150rpm. The conidia were separated by gauze filtration and washed with sterile 0.05M phosphate-buffered saline (PBS), at a pH of 7.2. An inoculum size (2 x 10⁶ CFU/animal) was ensured by cell counting in a Neubauer chamber and administered intraperitoneally in 0.2mL of PBS⁷. Inoculum viability was verified by plating a volume of the fungal cell suspensions on SDA immediately after inoculation and determining the number of colony-forming units after 7 days of incubation at 30^oC (minimum viability of 90%). Five groups of 10 animals/group were each infected with a strain of the different species studied, and a sixth control group with 10 animals received only 0.2mL of sterile saline. On the 14^th day after infection, all the animals were anesthetized with ketamine (100mg/kg) and xylazine (10mg/kg) and sacrificed by cervical dislocation. The liver and spleen were removed for ex vivo analysis and the organs weighed prior to homogenization in Tris-HCl buffer (pH 7.4, 10mM) at a ratio of 1:10w/v. The homogenates were centrifuged and the supernatants collected.

The activity of the antioxidant enzyme catalase in each of the organs was quantified by the spectrophotometric method of Aebi⁸, which measures the degradation of hydrogen peroxide (H₂O₂) in the presence of tissue homogenate supernatant (phosphate buffer pH 7.0, 25°C) at 240nm. The enzymatic activity is expressed as µmol H₂O₂/(min·g of tissue).

The total thiol content in each organ was measured by Ellman’s assay⁹. For the determination of non-protein thiol, trichloroacetic acid (TCA) was added to the organ homogenate supernatants, the contents were centrifuged at 3000rpm for 10 minutes, and the deproteinated supernatants were used for the analysis. Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) was added and the chromophore formed was measured spectrophotometrically at 412nm. The concentrations of protein and non-protein thiols were expressed as µmol protein thiol/g tissue and non-protein thiol/g tissue, respectively.

Inter-group comparisons were carried out by analysis of variance [(ANOVA) one-way] followed by post-hoc Tukey’s test, with the significance level set at 0.05.

The analysis of the liver and spleen of the mice infected with S. brasiliensis showed significant decreases in catalase activity in the liver (p=0.0042), protein thiol content in the liver (p<0.0001), and non-protein thiol content in the spleen (p<0.0001) (Figure 1). The liver weights showed no significant differences between the two groups. However, the mice infected with S. brasiliensis showed pronounced splenomegaly with significant increases in organ weight compared with either the control group or the mice infected with other species of Sporothrix (p<0.0001) (Figure 2). The other species also showed no significant differences compared with the control group in this regard.

FIGURE 1
Catalase activity and content of protein and non-protein thiol in the liver and spleen of animals infected with strains of the Sporothrix schenckii complex. aControl; b S. brasiliensis; c S. schenckii; d S. globosa; e S. mexicana; f S. albicans. S.: Sporothrix .

FIGURE 2
Liver and spleen weight of animals infected with strains of the Sporothrix schenckii complex. aControl; b S. brasiliensis; c S. schenckii; d S. globosa; e S. mexicana; f S. albicans. S.: Sporothrix .

The pathogenic mechanisms of Sporothrix are quite complex, showing intra and interspecies variability depending on the host immune response^5,10.

Comparative studies of virulence with species of the complex showed that the lesional mechanisms might be species specific, S. brasiliensis being the most virulent species, followed by Sporothrix schenckii, Sporothrix globosa, Sporothrix mexicana, and Sporothrix pallida². The authors found that S. brasiliensis showed a remarkable tropism for the brain, while for S. schenckii and S. globosa this tropism was lower.

In this study, the liver and kidney were the organs that showed greater vulnerability to oxidative stress caused by infection with Sporothrix, especially by S. brasiliensis.

Fernandes et al. observed a large heterogeneity in virulence profiles among different isolates of S. schenckii s. str. S. brasiliensis (CBS 132990) was the most virulent strain, leading to mortality within a short time of infection with a high fungal load³. In contrast, S. globosa (CBS 132922) was completely non-virulent with zero fungal load and survival of the animals at the end of the experiment³.

Some studies have investigated the protein secretion profile of Sporothrix spp. Fernandes et al. showed that Brazilian S. schenckii isolates express different proteins¹¹. It was also observed that the humoral response profiles of mice infected with S. schenckii s. str., S. globosa, and S. brasiliensis were distinct. The most virulent isolates (including S. schenckii s. str., and S. brasiliensis isolates), had in common 60-kDa and 110-kDa molecules, suggesting that these antigens could be involved in virulence³.

Reactive oxygen species are used by the host's immune system to eliminate an infectious agent by causing irreversible damage to their cellular components and eventually cell death. Cells of the host immune system, such as macrophages and neutrophils, can generate an oxidative burst following phagocytosis of fungal pathogens^5,12.

In the present study, we investigated catalase enzyme activity and levels of thiols (protein and non-protein), as these are markers that change rapidly in response to cellular oxidative stress. Catalase is an enzyme responsible for the degradation of the ROS hydrogen peroxide, which is highly toxic to the cell¹³. In the initial phase of an infection, the decrease in the levels of this enzyme may reflect an increase in the formation of hydrogen peroxide. The results presented indicate that this decrease occurred predominantly in the organs of animals infected with S. brasiliensis. Likewise, glutathione, a major cellular antioxidant also appears to be depleted by the antioxidation system of animals infected with S. brasiliensis as demonstrated by the significant reduction in the levels of protein and non-protein thiols in the liver and spleen, respectively.

Sporothrix brasiliensis is more resistant to peroxide stress than S. schenckii, which might be expected as S. brasiliensis is normally associated with the zoonotic outbreaks seen in Brazil. It is thought to be adapted to felines from which it can infect human hosts¹⁴. Interestingly, a recent study that showed plant pathogens are less resistant to peroxide stress than human fungal pathogens supports the idea that the species of the Sporothrix schenckii complex will display different adaptation strategies, and this will influence the likely route of infection¹⁵. These differences can also affect the resulting pathogenicity of each species in humans. For example, S. brasiliensis is known to be a more virulent pathogen than S. schenckii, but the latter species is always involved in infections resulting from traumatic inoculation via contaminated vegetal debris and soil.

Ethical considerations

The study was also approved by the Ethics Committee on Animal Use of the Federal University of Santa Maria, Brazil, prior to the start of experimentation (approval number: 006/2014).

REFERENCES

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  Ortega I, Felipe MSS, Vasconcelos ATR, Bezerra LML, Dantas AS. Peroxide sensing and signaling in the Sporothrix schenckii complex: an in silico analysis to uncover putative mechanisms regulating the Hog1 and AP-1 like signaling pathways. Med Mycol. 2015;53(1):51-9.

• Financial support: This study received funding from the Foundation for Research Support of the State of Rio Grande do Sul (FAPERGS).

Publication Dates

• Publication in this collection
  Jul-Aug 2017

History

• Received
  17 May 2016
• Accepted
  24 Apr 2017