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Revista da Sociedade Brasileira de Medicina Tropical

versão impressa ISSN 0037-8682versão On-line ISSN 1678-9849

Rev. Soc. Bras. Med. Trop. vol.52  Uberaba  2019  Epub 14-Jan-2019 

Short Communication

Morphological differentiation between seven Brazilian populations of Haemagogus capricornii and Hg. janthinomys (Diptera: Culicidae) using geometric morphometry of the wings

Shayenne Olsson Freitas Silva1  2  *

Ana Laura Carbajal de la Fuente3  *

Cecilia Ferreira de Mello1  4 

Jeronimo Alencar1 

1Laboratório de Diptera, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.

2Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemíptera, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.

3CONICET - Buenos Aires' University. Institute of Ecology, Genetics and Evolution (IEGEBA), Eco-Epidemiology Laboratory. Buenos Aires, Argentina.

4Instituto de Biologia, Pós-Graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brasil.



Haemagogus capricornii and Hg. janthinomys females are considered morphologically indistinguishable. We analyzed morphometric variability between Brazilian populations of these species using wing geometric morphometry.


Size and shape at intra- and interspecific levels were analyzed in 108 Hg. capricornii and Hg. janthinomys females.


Geometric morphometry indicated size and shape variables can differentiate these species at interspecific level. However, at intraspecific level, results show relative differentiation. Two populations of Hg. capricornii had a smaller centroid size with no significant differences between them, whereas all Hg. janthinomys populations showed significant differences.


Both species were correctly identified by geometric morphometry.

Keywords: Haemagogus capricornii; Haemagogus janthinomys; Culicidae; Geometric morphometry; Brazil

One of the most important genera of mosquitoes capable of infecting and transmitting the wild yellow fever virus (WFV) is Haemagogus Williston, 1896, which is considered a biological vector and responsible for maintaining the natural cycle of this zoonosis in forested areas of the Americas1. Mosquitoes of this genus are restricted to the Americas and almost all species have a Neotropical distribution, except for Hg. equinus Theobald, 1903, which can even be found in some southern parts of the Nearctic region2. These are mainly wild, diurnal, and acrodendrophic mosquitoes inhabiting primarily dense forest and gallery areas3.

Haemagogus is very diverse; it includes twenty-eight species of which nine are found in Brazil1. Some of these are epidemiologically important in the transmission of the wild-type yellow fever virus1. Among the nine known vector species, five stand out for the efficiency of their transmission in Brazil: Hg. albomaculatus Theobald, 1903, Hg. leucocelaenus Dyar and Shannon, 1924, Hg. spegazzini Bréthes, 1912, Hg. capricornii Lutz, 1904, and Hg. janthinomys Dyar, 1921. Larvae and females of Hg. capricornii and Hg. janthinomys species are currently morphologically indistinguishable, their differentiation being based primarily on characteristics of the male genitalia. Their identification is carried out based on the following: the presence (Hg. janthinomys) or absence (Hg. capricornii) of notable spiculosity on the ventral face of the aedeagus and the existence of a medial process, with a hooked shape, near the apex of the paraproct in Hg. janthinomys. These structures are small and only distinguishable by well-trained personnel and misidentifications can be frequent.

Although traditional morphometry contributed to the identification of these species, a more robust approach is necessary4. Geometric morphometry is a powerful, low-cost tool that addresses issues in taxonomy, ecology, and morphology, particularly in insects and especially in the family Culicidae, which possesses wings5. These bi-dimensional structures are eminently suitable for morphometrical description6. Geometric morphometry makes it possible to identify morphological variations and to explore their causes both within and between populations7. In Diptera, it has been widely used to answer questions mainly related to population studies6. A recent study of Culex mosquitoes from the state of Rio de Janeiro showed the effects of seasonal variations on phenotypic variations using this tool3.

Considering the difficulties in the identification of Hg. capricornii and Hg. janthinomys females, the poor knowledge about them, the partial overlap of their geographical distribution, and their eco-epidemiological importance, attention must be paid to the evaluation of old reports of infection of these and similar species, especially in Brazil1. In this context, the aim of this study was to determine the phenotypic variability in Hg. janthinomys and Hg. capricornii females at species and population levels, using the geometric morphometry of the wings. For this purpose, we included populations of the two species that have a large proportion of their geographic distribution in Brazil.

A total of 108 right and left wings of females belonging to Hg. capricornii and Hg. janthinomys from Brazil were used in this study (Figure 1A and Figure 1B; Table 1). The Haemagogus populations came from ecological and epidemiological studies carried out by the Diptera Laboratory team and from the Entomological Collection at the Oswaldo Cruz Institute, Fiocruz, Brazil. Species were identified by direct observation of morphological characters using an optical microscope (Leica DMD108® - Morrisville, United States of America - USA) according to Arnell (1973)1. Once identified, the wings were extirpated and later photographed according to Alencar et al. (2016)3.

FIGURE 1: Wings of Haemagogus janthinomys (A) and Hg. capricornii (B) with graph paper in the background. Landmarks (n = 14) are shown in (A). Gray bar = 1 mm. (C) Neighbor-joining trees derived from Mahalanobis distances of shape variables of Hg. capricornii and Hg. janthinomys females from Brazil. (Populations as in Table 1). 

TABLE 1: Geographical location, coordinates, altitude, origin, and number of wigs (N = 108) of the sampled females of Haemagogus janthinomys and Hg. capricornii populations from Brazil. 

Species Locality/ State Population code Wings (N) Latitude Longitude Altitude (m)
Hg. janthinomys Atalaia/Alagoas Hgj_Ata 9 -9.538056 -36.132778 54
Jacarandá/Bahía Hgj_Jac 27 -15.863056 -38.882778 8
Canavieiras/Bahía Hgj_BA 4 -15.675000 -38.947222 4
Campina Verde/Minas Gerais Hgj_Cav 18 -19.538611 -49.486389 494
Duque de Caxias/Rio de Janeiro Hgj_RJ 19 -22.785556 -43.311667 19
Hg. capricornii Duque de Caxias/Rio de Janeiro Hgc_RJ 10 -22.578611 -43.314722 24
Mangarí/Minas Gerais Hgc_Man 21 -18.587222 -46.514444 950

Fourteen type-1 landmarks were selected and included in the analyses8. We used coordinate data and the isometric estimator centroid size (CS) to compare overall wing sizes between species and populations. The Mann-Whitney test was applied to comparisons of CS between species and populations. The shape variables (partial warps and uniform components) were obtained using the generalized Procrustes analysis superimposition algorithm. Mahalanobis distances derived from the shape variables were used to explore shape proximity between the species and populations. Statistical significance was determined by permutation tests (1,000 runs each) and corrected by the Bonferroni method.

We represented the Mahalanobis distances between species and populations in neighbor-joining (NJ) trees. The percentage of phenotypic similarity between species and populations was calculated using the cross-check test of discriminant analysis. Shape variables were regressed onto CS by multivariate regression analysis to detect allometry. The correlation between geographic and Mahalanobis distances was determined by a Mantel test (1,000 permutations) using straight-line geographic distances between collection sites as described by Rosenberg and Anderson (2011)9.

The geometric coordinates of each landmark were digitalized using the program tpsDig version 2.09 (available at Centroid size generalized Procrustes analysis, Mahalanobis distances, permutation tests, and allometry were performed using the modules VAR, MOG, PAD, and COV respectively, included in the CLIC98 package, according to Dujardin 200810. The correlation between geographic and Mahalanobis distances was determined by Mantel tests using the PASSaGE 2 software (available at

For interspecific comparison, the size variable revealed that the centroid size of Hg. capricornii was significantly smaller (Mann-Whitney test, P = 0.01) than Hg. janthinomys. The permutation test based on the Mahalanobis distances revealed significant differences for shape variables between the two species (P = 0.01). The "cross-checked classification" of Hg. capricornii and Hg. janthinomys individuals showed that 81% and 67%, respectively, of all specimens were correctly assigned.

For intraspecific comparison, the size variable, revealed that all populations of Hg. janthinomys were significantly different among themselves and bigger than Hg. capricornii (Mann-Whitney test, P = 0.01). However, the analysis of populations of Hg. capricornii showed no significant differences among them (P = 0.06). The permutation test based on the Mahalanobis distances revealed significant differences for shape variables among some populations (Table 2). The contribution of the canonical factors resulted from 38%, 26%, and 15% for the first, second, and third factors, respectively. A "cross-checked classification" of individuals of the seven populations of Hg. capricornii and Hg. janthinomys showed acceptable and heterogeneous reclassification scores. Hg. capricornii populations showed low reclassification scores (30-42%). Although very heterogeneous, populations of Hg. janthinomys had better reclassification scores, from low (22% Bahía), to medium (42% Rio de Janeiro), to high (77% Atalaia, Rio de Janeiro). The NJ tree based on the distances of Mahalanobis showed that the two populations of Hg. capricornii (Hgc_RJ, Hgc_Man) were the most similar, followed by the Hg. janthinomys (Hgj_RJ) population, and morphologically different from the population of Campina Verde (Hgj_CaV) (Figure 1C). In addition, the NJ tree showed that the Hg. janthinomys populations (Hgj_Ata, Hgj_Jac) were different from the Bahia population (Hgj_BA). The Mantel test revealed a positive and significant association between the geographic distances and distances of Mahalanobis (r = 0.467; P = 0.01). A multivariate regression analysis of shape variables on the size variable showed no significant effect (test after 1000 permutations, P = 0.11).

TABLE 2: Mahalanobis distances for wings of females of Haemagogus janthinomys and Hg. capricornii from four states in Brazil. 

Species Mahalanobis distances
Code Hgj_Ata Hgj_Jac Hgj_BA Hgj_CaV Hgj_RJ Hgc_RJ Hgc_Man
Haemagogus janthinomys Hgj_Ata 0.00
Hgj_Jac 3.60* 0.00
Hgj_BA 5.02 3.30 0.00
Hgj_CaV 5.41* 3.74* 4.38 0.00
Hgj_RJ 4.54* 1.89 3.54 3.73* 0.00
Haemagogus capricornii Hgc_RJ 5.32* 3.86* 4.20 5.44* 3.57* 0.00
Hgc_Man 4.55* 2.48* 3.70 4.17* 2.32 3.62* 0.00

Hgj_Ata: Atalaia/Alagoas; Hgj_Jac: Jacarandá/Bahía; Hgj_BA: Canavieiras/Bahía; Hgj_CaV: Campina Verde/Minas Gerais; Hgj_RJ: Duque de Caxias/Rio de Janeiro; Hgc_RJ: Duque de Caxias/Rio de Janeiro; Hgc_Man: Mangarí/Minas Gerais. *Distances were significant at P < 0.0033 after Bonferroni correction.

Our results based on wing geometric morphometry of Hg. capricornii and Hg. janthinomys indicate that both size and shape variables can differentiate at the interspecific level. However, at the intraspecific level, the results show a relative differentiation. The two populations of Hg. capricornii had a smaller centroid size with no significant difference between them, whereas all Hg. janthinomys populations showed significant differences. The shape variables were able to separate the two Hg. capricornii and Hg. janthinomys populations, except for the two originating in Bahia, which were not statistically different.

The importance of taxonomy in biological sciences is undeniable. Biodiversity mapping should focus on limited groups so that research that is more thorough can be carried out effectively. Our results are congruent with the hypothesis that suggests Hg. capricornii and Hg. janthinomys may constitute a complex of species whose morphological differentiation is complex. To help identify these cryptic species and to study the relationship between them, new tools, such as molecular biology and biochemistry, have been used in addition to morphological methods, such as classical morphology, scanning electron microscopy, and morphometry11.

Modern molecular tools are available to discriminate between sister species living in sympatry12. However, they are expensive to use and require specialized training. Geometric morphometrics have been shown to be highly informative, fast, and affordable. With minimal training, geometric morphometry can be used to answer ecological or taxonomic questions6. This study demonstrates that geometric morphometry can discriminate with considerable success Hg. capricornii and Hg. janthinomys females that cannot be identified by traditional morphological criteria.

Although centroid size is not a good measure to use in species identification because it is affected by environmental factors, our results show that this size variable was able to differentiate between the two species13. Thus, conformation is a reasonably good feature to solve identification problems and is merely affected by the environmental factors14. Our study was able to differentiate between the two species, as well as between some populations. The correlation analysis between centroid size and the shape variables for Hg. capricornii and Hg. janthinomys did not show a common allometric slope. The association between the shape of the kites and the geographic distance between the populations suggests that the morphological variation could fit a distance isolation model.

Our study had some limitations. The results were obtained from a limited number of individuals and samples were more abundant for populations of Hg. capricornii than Hg. janthinomys. This type of problem is frequent in works that present data that involve field collections.

Our results support the use of geometric morphometry in the morphological discrimination of Hg. capricornii and Hg. janthinomys females. Proper identification of species is the fundamental basis for building knowledge of biodiversity, ecology, and other areas of biology. Failures in species identification may lead to the diffusion and amplification of conceptual and methodological errors in other areas, with implications not only for our knowledge of nature, but also for ecosystem structure functioning, management decisions, and human health vector control programs15. Correct species identification using geometric morphometry could contribute to improving vector control strategies.


1. Arnell JH. Mosquito studies (Diptera, Culicidae). XXXII. A revision of the genus Haemagogus. Contrib Am Entomol Inst 1973;10:1-174. [ Links ]

2. Forattini OP. Medical Culicidology, Identification, Biology, Epidemiology. 2° Vol. University of São Paulo Publisher, São Paulo, Brasil; 2002.860 p. [ Links ]

3. Alencar J, Mello CF, Gil-Santana HR, Guimarães AE, Almeida SAS & Gleiser RG. Vertical oviposition activity of mosquitoes in the Atlantic Forest of Brazil with emphasis on the sylvan vector, (Diptera: Culicidae). J Vector Ecol. 2016;41:18-26. [ Links ]

4. Alencar J, Serra-Freire NM, Marcondes CB, Silva JS, Correa FF & Guimarães AE. Influence of climatic factors on the population dynamics of Haemagogus janthinomys (Diptera: Culicidae), a vector of sylvatic yellow fever. Entomol News. 2010;121:45-52. [ Links ]

5. Adams DC, Rohlf FJ & Slice DE. A field comes of age: geometric morphometrics in the 21st century. Hystrix. 2013;24:7-14. [ Links ]

6. Lorenz C, Almeida F, Almeida-Lopes F, Louise C, Pereira SN, Petersen V & Suesdek L. Geometric morphometrics in mosquitoes: what has been measured? Infect Genet Evol. 2017;54:205-15. [ Links ]

7. Lawing AM & Polly PD. Geometric morphometrics: recent applications to the study of evolution and development. J Zool. 2010;280(1):1-7. [ Links ]

8. Bookstein FL. Morphometric tools for landmark data: geometry and biology. Cambridge: Cambridge Univ. Press; 1991. 198 p. [ Links ]

9. Rosenberg MS, & Anderson CD. Passage: Pattern Analysis, Spatial Statistics and Geographic Exegesis. Version 2. MEE. 2011;2:229-32. [ Links ]

10. Dujardin JP. Morphometrics applied to medical entomology. ‎Infect Genet Evol . 2008;8:875-90. [ Links ]

11. Forattini OP. Culicidiologia Médica. São Paulo: University of São Paulo Publisher, São Paulo, Brasil; 1996. 860 p. [ Links ]

12. Jaramillo-O N, Dujardin JP, Calle-Londoño D, Fonseca-González I. Geometric morphometrics for the taxonomy of 11 species of Anopheles (Nyssorhynchus) mosquitoes. Med Vet Entomol. 2015;29:26-36. [ Links ]

13. Gómez GF, Márquez EJ, Gutiérrez LA, Conn JE & Correa MM. Geometric morphometric analysis of Colombian Anopheles albimanus (Diptera: Culicidae) reveals significant effect of environ- mental factors on wing traits and presence of a metapopulation. Acta Trop. 2014;135:75-85. [ Links ]

14. Zelditch ML, Swiderski DL & Sheets HD. Geometric morphometrics for biologists: A Primer. Elsevier Academic Press, London and New York, NY; 2004. 437 p. [ Links ]

15. Bortolus A. Error cascades in the biological sciences: the unwanted consequences of using bad taxonoomy in ecology. AMBIO. 2008;37(2):114-8. [ Links ]

Financial Support: We would like to acknowledge Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro, FAPERJ (26/010.001630/2014; E-26/202.819/2015) and Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (301707/2017-0).

Recebido: 19 de Março de 2018; Aceito: 06 de Junho de 2018

Corresponding author: Jeronimo Alencar.

* Authors with equal contributions.

Conflict of Interest: The authors declare that there is no conflict of interest.

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