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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682On-line version ISSN 1678-9849

Rev. Soc. Bras. Med. Trop. vol.53  Uberaba  2020  Epub Jan 27, 2020

http://dx.doi.org/10.1590/0037-8682-0299-2019 

Short Communication

New report of Eratyrus cuspidatus Stål, 1859 (Hemiptera: Reduviidae: Triatominae) in the State of Campeche, Mexico

Paulino Tamay-Segovia1 
http://orcid.org/0000-0001-5329-0476

Selene Blum-Domínguez2 

Ricardo Alejandre-Aguilar3 

Luis Alberto Núñez-Oreza4 

Betty Sarabia-Alcocer5 

Vicente Jesús Chan-Puc6 

1Laboratorio de Enfermedades Transmitidas por Vectores y Zoonosis. Centro de Investigaciones Biomédicas de la Universidad Autónoma de Campeche. Campeche, México.

2 Laboratorio de Enfermedades Tropicales, Centro de Investigaciones Biomédicas de la Universidad Autónoma de Campeche. Campeche, México.

3 Laboratorio de Entomología, Depto. de Parasitología Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional. Ciudad de México, México.

4 Laboratorio de Microbiología y Biología Molecular, Centro de Investigaciones Biomédicas de la Universidad Autónoma de Campeche. Campeche, México.

5 Facultad de Medicina de la Universidad Autónoma de Campeche. Campeche, México.

6 Facultad de Enfermería de la Universidad Autónoma de Campeche. Campeche, México.


Abstract

INTRODUCTION:

Triatomine bugs are vectors of Trypanosoma cruzi, the etiological agent of Chagas disease.

METHODS:

Triatomine bugs were collected and identified following established protocols. In addition, infection with T. cruzi was detected by microscopic and molecular analysis.

RESULTS:

We captured an adult male specimen of the Eratyrus cuspidatus species that has not been reported in the state of Campeche.

CONCLUSIONS:

This finding provides new information on the distribution of E. cuspidatus in Mexico. However, more studies are needed to determine their epidemiological significance.

Keywords: Triatominae; Eratyrus cuspidatus; Sylvatic vectors

Triatomine bugs are important vectors of Trypanosoma cruzi (Chagas, 1909), the etiological agent of Chagas disease, which is endemic across much of the Americas. According to the Pan American Health Organization, approximately 6 million people are infected and 65 million are at risk of contracting the infection1. Currently, 150 extant and 2 extinct species of triatomines are known, and grouped into 16 genera2. In Mexico, approximately 33 triatomine (Hemiptera: Reduviidae: Triatominae) species have been reported, so far. It is therefore, one of the countries with the greatest diversity of triatomines. Two genera namely, Triatoma (20 species) and Meccus (6 species) are the most abundant and widely distributed3. However, other species, that until recently, had not been considered epidemiologically significant have also been reported3: Dipetalogaster maxima (Uhler, 1894), Eratyrus cuspidatus (Stål, 1859), Paratriatoma hirsuta (Barber, 1938), Panstrongylus rufotuberculatus (Champion, 1899), Triatoma nitida (Usinger, 1939), and Rhodnius prolixus (Stål, 1859). E. cuspidatus was found to be naturally infected with T. cruzi4,5,6. They have begun to invade and colonize human dwellings. Since they transmit the etiologic agent of Chagas disease, more knowledge is needed about their distribution and ecology, to improve control strategies.

E. cuspidatus is distributed throughout Colombia, Ecuador, Guatemala, Mexico, Panama, Peru, and Venezuela3. In Mexico, it has been observed only in the three states: Chiapas, Veracruz, and Yucatan3,7,8,9. In the state of Campeche, Triatoma dimidiata (Latreille, 1811) is the only species associated with T. cruzi transmission, since the last 70 years4. However other vector species have been identified in neighboring states. It is therefore, likely that more than one vector is present in Campeche3,7.

Reports of sylvatic species of triatomines, contribute to the knowledge about current and new species distributions, and describe their role as existing or possible vectors of T. cruzi.

The state of Campeche is located within the following geographic coordinates: 17°48’46” N to 20°50’53” N and 92°28’7” W to 89°07’16” W. It is bordered to the north by the Gulf of Mexico and Yucatan, to the east by Quintana Roo and Belize, to the south by the Republic of Guatemala and the state of Tabasco, and to the west by the state of Tabasco and the Gulf of Mexico. The average annual temperature throughout the state is 26 °C while that along the coast is 28 °C10.

In March 2010, a study was conducted in Calakmul, a protected, natural, rainforest reserve, located in the Calakmul municipality, approximately 179.5 km from the capital city (Campeche). Triatomine bugs were collected manually, during the day, and with a lamp at night, according to the method described by Schofield11. During the search, we examined all possible ecotopes (including hollow trees and cracks, holes in the ground, and stone piles). Captured specimens were placed in labeled flasks, containing folded cardboard sheets, and transported to the laboratory for morphological identification according to the key described by Lent and Wygodzinsky4. Infection with flagellates was determined by microscopic observation of feces, obtained after abdominal compression, and dilution in phosphate buffered saline (PBS). Presence of T. cruzi was determined by PCR12.

Primarily, adults of T. dimidiata infected with T. cruzi were collected. Although this species was the most abundant, one adult of another triatominae species was collected. This specimen was probably attracted to the lights and consequently, captured near the bedroom, of the home of forest guards (18°21'54'' N and 089°53'32'' W) and visitors. It was identified as a male of E. cuspidatus4, which presents rounded discal tubercles, humeral angles angular or pointed but not spinose, and the process of the scutellum bent slightly upward (Figure 1). In contrast E. mucronatus presents discal tubercles in the form of strong spines, humeral angles distinctly spinose, and the scutellum upward in variable angle. The authors also compared the specimen with T. dimidiata, the more abundant vector in the area. T. dimidiata presents discal tubercles that are not pointed and not elevated, humeral angles rounded, a scutellum with a central area that is not depressed, and an apical process that is subcylindrical and bent slightly downward at the apex (Figure 2B). Identification of E. cuspidatus was confirmed by the entomologist, Alejandre-Aguilar, expert in triatomines, at the Laboratory of Entomology of the Escuela Nacional de Ciencias Biologicas del Instituto Politecnico Nacional.

FIGURE 1: Lateral view of a male specimen of Eratyrus cuspidatus, length 23mm. 

FIGURE 2: Dorsal view of two male triatomine specimens: (A) Eratyrus cuspidatus, length 23mm. and (B) Triatoma dimidiata, 26mm. 

The most important epidemiological species are those that live among humans and have a strong preference for human blood11. However, sylvatic species are a source of infestation and re-infestation; in some cases, they even replace domestic species that had been controlled with chemical products (insecticides). When they adapt to the type of habitat and fauna, they can establish an ecological chain of epidemiologically important vector species13. Thus, E. cuspidatus could contribute to the transmission of T. cruzi in humans, as reported in other countries14.

In addition to T. dimidiata reported by Valdez-Tah et al.15, the occurrence of E. cuspidatus in proximity to a human dwelling suggests, that it may eventually become a part of the transmission cycle of T. cruzi among the human communities established in these areas.

This study reports the occurrence of a particular species that had not been previously reported in the state. It also reports the recent proximity of this species to human environments, possibly as a consequence of human movement into sylvatic areas.

We examined E. cuspidatus for T. cruzi infection. However, no flagellates were detected. Similarly, the result of PCR was also negative for the presence of T. cruzi. Thus, our findings concur with other reports of E. cuspidatus in Mexico. However, it is yet unknown, whether they are limited to sylvan areas, with occasional intrusion into human dwellings, or if they could possibly invade domestic areas. Therefore, more studies are needed to further investigate and determine the importance of these species as vectors of T. cruzi.

ACKNOWLEDGEMENTS

To Carlos Jesús Maas Nolasco for the photographs that appear in this work.

REFERENCES

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14. Ayala Hoyos CJ, Hernández Mendoza CM, Eyes Escalante M, Romero Ricardo LR, Álvarez Rodríguez RA, Blanco Tuirán P. Detección de infección natural por Trypanosoma cruzi (Trypanosomatidae) en triatominos del municipio de Colosó, Colombia. Acta biol. Colomb. 2019;24(1):180-4. DOI: http://dx.doi.org/10.15446/abc.v24n1.72306 [ Links ]

15. Valdez-Tah A, Huicochea-Gómez L, Ortega-Canto J, Nazar-Beutelspacher A, Ramsey JM. Social Representations and Practices Towards Triatomines and Chagas Disease in Calakmul, México. Plos One. 2015;10(7):1-28. [ Links ]

Financial Support: This study was funded by the Autonomous University of Campeche Project number UACAM-EXB-065-2009.

Received: June 17, 2019; Accepted: October 30, 2019

Corresponding author: Dr. Paulino Tamay-Segovia. e-mail:pautamay@hotmail.com

Authors’ contribution: TSP: Capture of triatomines and molecular analysis of DNA samples and report writing; BDS: Capture of triatomines and extraction of genetic material (DNA) and report writing; AAR: Identification and confirmation of triatomine species; NOLA: Translation into English and report writing. SAB: Capture of triatomines and microscopic analysis of infection with Trypanosoma cruzi; CPVJ: Capture of triatomines and microscopic analysis of infection with Trypanosoma cruzi.

Conflict of interest: The authors declare no conflict of interest regarding the publication of this manuscript.

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