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Revista da Sociedade Brasileira de Medicina Tropical

Print version ISSN 0037-8682On-line version ISSN 1678-9849

Rev. Soc. Bras. Med. Trop. vol.53  Uberaba  2020  Epub Mar 16, 2020

https://doi.org/10.1590/0037-8682-0436-2019 

Short Communication

First report of Rhodnius montenegrensis (Hemiptera, Reduviidae, Triatominae) in Amazonas, Brazil

Fernanda Portela Madeira1  2 

André Luiz Rodrigues Menezes3 

Adila Costa de Jesus1  2 

Madson Huilber da Silva Moraes1 

Jader de Oliveira4  5 

João Aristeu da Rosa4  5 

Luís Marcelo Aranha Camargo1  6  7  8  9 

Dionatas Ulises de Oliveira Meneguetti1  10 

Paulo Sérgio Berrnarde1  2 
http://orcid.org/0000-0002-2191-7817

1Universidade Federal do Acre, Programa de Pós-Graduação Stricto Sensu em Ciências da Saúde na Amazônia Ocidental, Rio Branco, AC, Brasil.

2Universidade Federal do Acre, Campus Floresta, Centro Multidisciplinar, Laboratório de Herpetologia, Cruzeiro do Sul, AC, Brasil.

3Instituto Federal de Rondônia, Campus de Guajará- Mirim, RO, Brasil.

4Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Farmacêuticas, Departamento de Ciências Biológicas, Araraquara, SP, Brasil.

5Universidade Estadual Paulista Júlio de Mesquita Filho, Programa de Pós-Graduação Stricto Sensu em Biociências e Biotecnologia, Araraquara, SP, Brasil.

6Universidade de São Paulo, Instituto de Ciências Biomédicas 5, Monte Negro, RO, Brasil.

7Centro Universitário São Lucas, Departamento de Medicina, Porto Velho, RO, Brasil.

8Centro de Pesquisa em Medicina Tropical de Rondônia, Porto Velho, RO, Brasil.

9Instituto Nacional de Epidemiologia da Amazônia Ocidental, Porto Velho, RO, Brasil.

10Universidade Federal do Acre, Colégio de Aplicação, Rio Branco, AC, Brasil.


Abstract

INTRODUCTION:

Triatomines are hematophagous insects of epidemiological importance because they are vectors of Chagas disease. We present here the first report of Rhodnius montenegrensis in Amazonas, Brazil.

METHODS:

Triatomines were collected from Attalea butyracea palm trees in the municipality of Guajará.

RESULTS:

Two adult female R. montenegrensis specimens were identified.

CONCLUSIONS:

The present study confirms that the number of triatomine species within the Amazon has increased from 10 to 11, and the number of Brazilian states with R. montenegrensis has increased from two to three.

Keywords: Western Amazon; Chagas disease; Triatomines

Triatomines are hematophagous insects belonging to the Reduviidae family and the Triatominae subfamily1. They are found throughout South and Central America and are of epidemiological importance as they are vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease (American trypanosomiasis)1. These vectors may also transmit the protozoan, Trypanosoma rangeli, to vertebrates; although this species does not cause symptoms of infection in humans, it may complicate differential diagnosis of T. cruzi2.

Currently, 154 triatomine species, grouped into 19 genera3-5, have been identified worldwide. Of these, over 30 species, distributed among nine genera, occur within the Amazon region6. In the Brazilian state of Amazonas, ten species, distributed among four genera, were previously recorded: Cavernicola lenti Barrett and Arias, 1985, Eratyrus mucronatus Stal, 1859, Panstrongylus geniculatus (Latreille, 1811), P. lignarius (Walker, 1873), P. rufotuberculatus (Champion, 1899), Rhodnius amazonicus Almeida, Santos, and Sposina, 1973, R. brethesi Matta, 1919, R. paraensis Sherlock, Guitton and Miles, 1977, R. pictipes Stal, 1872 and R. robustus Larrousse, 19277. The present article reports the first occurrence of R. montenegrensis in the Brazilian state of Amazonas.

In January 2019, using a dissection technique, triatomines were collected from four Attalea butyracea palm trees (commonly referred to as Jaci or coquinho da mata in the Amazon region) in a rural area of the municipality of Guajará, Amazonas, near the river Juruá (latitude 07º 30' 87''S, longitude 72º31'17''W). This municipality is located in the meso-region of the southwestern Amazon and the micro-region of Juruá.

Two triatomines were collected and transferred to the Laboratory of Tropical Medicine at the Federal University of Acre (UFAC) in the city of Rio Branco, Acre, where they were identified based on their morphological characteristics, previously described by Lent and Wygodzinsky8 and Rosa et al.9. These two specimens were subsequently identified as female R. montenegrensis and referred to the Entomology Laboratory of the Department of Biological Sciences, School of Pharmaceutical Sciences, Paulista State University Júlio de Mesquita Filho (UNESP), Araraquara, São Paulo, Brazil, where the species were further confirmed by genital charactersistics10 (Figure 1A-D). These R. montenegrensis individuals (two adult females, municipality of Guajará, Amazonas, Brazil, latitude 07º 30' 87''S, longitude 72º31'17''W, Madeira, F.P, col. Oliveira J, det.) were then added to the Triatominae collection, "Dr Jose Maria Soares Barata" (CTJMSB) of the UNESP, Araraquara.

Collected R. montenegrensis individuals were mainly yellow in color with black longitudinal stripes on the pronotum, wings, and connexivum9 (Figure 1A). Markings on the head included a distinct central yellow band between two continuous brown bands; stains from the climax regions to the neck were not present9 (Figure 1B). Legs were yellow in color, except for the posterior tibias, which had a black stripe near the tarsus9(Figure 1A).

FIGURE 1: Female specimen of Rhodnius montenegrensis collected from Attalea butyracea palm trees in the municipality of Guajará, Amazonas, Brazil. (A) Dorsal view. (B) Head. (C) Detail of the pronotum. (D) Ventral view. (E) Female genitalia. 

R. montenegrensis specimens were further analyzed for Trypanosoma sp. infection. Fresh intestinal content in a 0.9% saline solution was examined using optical microscopy (400x magnification)11. R. montenegrensis specimens were not positive for trypanosomatids, however.

This is the first report of R. montenegrensis in Amazonas. This species was first described in the municipality of Monte Negro, Rondônia9, and it was further recorded in two meso-regions in the state of Acre1,12. Given the results of the present report, the number of triatomines occurring in the state of Amazonas has increased from 10 to 11, expanding the distribution of this species in Brazil, which previously only comprised the states of Rondônia and Acre (Figure 2).

FIGURE 2: Distribution of Rhodnius montenegrensis in Brazil. 

Although in the present study, R. montenegrensis specimens were not infected by trypanosomatids, this species is nonetheless a potential vector for transmission of this etiological agent given that its infection by T. cruzi13 and T. rangeli2 was previously confirmed. R. montenegrensis individuals infected by T. rangeli were further found inside apartments in the state of Acre, but without evidence of domiciliation14.

The occurrence of T. cruzi-infected R. montenegrensis individuals indicates that this triatomine has an active role in the maintenance of the enzootic cycle of this trypanosomatid13,15, thus reinforcing the need for further studies on R. montenegrensis occurrence to further elucidate its distribution and overall ecology.

ACKNOWLEDGEMENTS

The authors would like to thank the Acre State Research Support Foundation (FAPAC) and the Dean of Research and Post-Graduation (PROPEG) at the Federal University of Acre (UFAC).

REFERENCES

1. Meneguetti DUO, Tojal SD, Miranda PRM, Rosa JA, Camargo LMA. First report of Rhodnius montenegrensis (Hemiptera, Reduviidae, Triatominae) in the State of Acre, Brazil. Rev Soc Bras Med Trop. 2015;2015(48):471-3. [ Links ]

2. Meneguetti DUO, Soares EB, Campaner M, Camargo LMA. First report of Rhodnius montenegrensis (Hemiptera, Reduviidade, Triatominae) infection by Trypanosoma rangeli. Rev Soc Bras Med Trop . 2014;47(3):374-6. [ Links ]

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12. Jesus AC, Madeira FP, Moraes MHS, Morais AA, Moresco GG, Oliveira J, et al. Increased geographical distribution of Rhodnius stali and Rhodnius montenegrensis: First report in the Juruá Valley region, Acre, Brazil. In: Salgado YCS, Oliveira, AC, editors. Pathologies: parasitic diseases. 1st ed. Ponta Grossa: Athena; 2019. p. 25-34. [ Links ]

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14. Ribeiro MAL, Castro GVS, Souza JL, Rosa JA, Camargo LMA, Meneguetti DUO. Occurrence of triatomines in an urban residential complex in the municipality of Rio Branco, Acre, South-Western Amazon. Rev Soc Bras Med Trop. 2019;52(1):1-4. [ Links ]

15. Bilheiro AB, Oliveira J, Bellintani T, Fontes G, Junior A, Meneguetti DUO, et al. Biological aspects of Rhodnius montenegrensis (Hemiptera, Reduviidae, Triatominae) under laboratory conditions. Vector Borne Zoonotic Dis . 2019;20(20):1-4. [ Links ]

Ethical considerations: Triatomine collection was carried out under a permanent license issued by the Brazilian Institute of the Environment and Renewable Natural Resources (IBAMA) (License no. 52260-1).

Financial support: This study was supported by the Research Program for the Single Health System (SUS): Shared Health Management (PPSUS) of the Acre State Research Support Foundation (FAPAC).

Received: September 16, 2019; Accepted: December 12, 2019

Corresponding author: Dr. Paulo Sergio Bernarde. e-mail:SnakeBernarde@hotmail.com

Conflicts of interest: The authors declare that there is no conflict of interest.

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License