Print version ISSN 0041-8781
Rev. Hosp. Clin. vol.58 no.6 São Paulo 2003
A critical review of the possible benefits associated with homeopathic medicine
Uma revisão crítica da literatura relativa aos possíveis benefícios da medicina homeopática
Renan Moritz V. Rodrigues Almeida
Federal University of Rio de Janeiro - Program of Biomedical Engineering (COPPE) - Rio de Janeiro/RJ, Brazil
OBJECTIVE: To evaluate the recent scientific research progress on homeopathy.
METHODOLOGY: Homeopathy was evaluated in terms of its clinical research; in vitro research, and physical foundations. The Medline database was the main reference source for the present research, concerning data of approximately the last 10 years. Secondary references (not available in this database) were obtained by means of direct requests to authors listed in the primary references.
RESULTS: Clinical studies and in vitro research indicate the inefficacy of homeopathy. Some few studies with positive results are questionable because of problems with the quality and lack of appropriate experimental controls in these studies. The most recent meta-analyses on the topic yielded negative results. One of the few previous meta-analyses with positive results had serious publication bias problems, and its results were later substantially reconsidered by the main authors. The sparse in vitro homeopathic research with positive results has not been replicated by independent researchers, had serious methodological flaws, or when replicated, did not confirm the initial positive results. A plausible mechanism for homeopathic action is still nonexistent, and its formulation, by now, seems highly unlikely.
CONCLUSIONS: As a result of the recent scientific research on homeopathy, it can be concluded that ample evidence exists to show that the homeopathic therapy is not scientifically justifiable.
Descriptors: Homeopathy. Complementary and alternative medicine. Pseudo-science.
OBJETIVOS: Avaliar os resultados da pesquisa científica em relação aos possíveis benefícios da homeopatia.
METODOLOGIA: A homeopatia foi avaliada a partir de sua pesquisa clínica; sua pesquisa in vitro ou "pré-clínica" e seus fundamentos físicos. Para tal, foi realizada uma ampla revisão e análise crítica da literatura científica mais recente no tópico. (aproximadamente últimos dez anos). Os trabalhos foram selecionados primeiramente a partir da base de dados Medline. Referências secundárias (não indexadas nesta base de dados) puderam então ser obtidas por meio de contato direto com autores listados nas referências primárias.
RESULTADOS: Tanto estudos clínicos quanto pesquisas in vitro e estudos de "provas" homeopáticas apontam para a ineficácia de sua utilização. Alguns poucos estudos com resultados positivos podem ser explicados por problemas de qualidade e (falta de) controles adequados. As meta-análises mais importantes na área forneceram resultados negativos. Uma recente meta-análise com resultados positivos teve sérios problemas metodológicos, e seus resultados foram, posteriormente, substancialmente alterados pelos autores. A pesquisa metodológica in vitro, similarmente, apresenta poucos resultados positivos, os quais possuem sérios problemas metodológicos e não puderam ser replicados por pesquisadores independentes. Um mecanismo plausível para a atuação das substâncias homeopáticas é inexistente, e sua formulação, atualmente é altamente improvável.
CONCLUSÃO: Como resultado das pesquisas realizadas nos últimos anos, podemos concluir que existe ampla evidência de que a prática homeopática não é cientificamente justificável.
Descritores: Homeopatia. Medicina complementar e alternativa. Pseudo-ciência.
Homeopathy, introduced by the German physician Samuel Hahnemann at the beginning of the 19th century, consists of the treatment of the symptoms of a disease by means of ultra-diluted substances. These substances are chosen based on the assumption that they would cause the same symptoms in healthy subjects if ingested in measurable amounts. Minerals, vegetables, or substances obtained from living organisms may be used in the preparation of homeopathic medicines. Supposedly, the effect of these substances becomes stronger the more they are diluted. An important step in their preparation is called the succussion: "shakes" that are applied to the substance after each dilution stage. Although mostly recommended for small afflictions, sometimes radical statements about the effectiveness of homeopathy are made, for instance, concerning the treatment of AIDS1.
In Brazil, homeopathy is accepted as a medical, pharmaceutical, and veterinary specialty. Homeopathy also has an accepted medical status in England, India, and some States of the United States (Arizona, Connecticut, and Nevada), and its use is widespread in many other countries2. The amount of research on homeopathic topics rose substantially in the last 20 years. The analysis of its scientific status, therefore, can already take into account a large number of studies, and such an analysis is obviously important, both in terms of general scientific advancement and for health financing reasons.
The objective of the present study was to critically review and assess the current scientific status of homeopathic medicine. This review focused on the performance of homeopathy in clinical trials, on its state-of-the-art in basic research, and on its physical foundations. References were basically obtained with the help of the Medline-Pubmed database and were restricted to (approximately) the last 10 years. Secondary references, not available in this database, were also identified by means of direct contact with primary authors.
Probably the best strategy for assessing the effect of homeopathy is through the results of its clinical trials. Many good quality studies of the last 12 years have yielded negative results, for instance, concerning systematic reviews and meta-analyses3-11; infections of the upper respiratory tract and allergic rhinitis12,13; post-operative ileus14; asthma15; warts16-18; pain relief and muscle soreness19-23; treatment of adenoid vegetation24; chronic headache25-28; ear inflammation29,30; eczema31; arthritis32,33; bruising34,35; anxiety36; "provings" (see below) with belladonna, arnica, Pulsatilla and mercury37-41 and "aggravations" ("good" worsening of symptoms, said by homeopaths to precede patient recovery)42.
Detailed comments on many of these studies are published2,5,43. Some studies were at first considered to be of good methodological quality, in which positive results were found, concerning, for example, allergic rhinitis and childhood diarrhea44,45. These studies, however, could not be independently replicated13,46, and some methodological flaws were later detected in their designs47,48. The present review could not identify any homeopathic clinical trial that was positively replicated by an independent group49,50.
In 1990, Hill and Doyon3 reviewed 40 randomized double-blind studies, and concluded that "The results do not provide acceptable evidence that homeopathic treatments are effective". In a review of the scientific literature concerning pediatric homeopathy, Kurz4 concluded that "The review of studies carried out according to current scientific criteria revealed-at besta placebo effect of homeopathy". Linde and Melchart6, in a review of homeopathic studies on "individualized homeopathy" (one of the current schools of homeopathic treatment), stated that " when the analysis was restricted to the methodologically best trials no significant effect was seen". Ernst and Pittler7 in a review of the scientific literature on the homeopathic preparation - arnica, concluded that "The claim that homeopathic arnica is efficacious beyond a placebo effect is not supported by rigorous clinical trials". One of these authors, in another review8, states: "It is concluded that the trial data available to date do not suggest that homeopathy is effective in the prophylaxis of migraine or headache beyond a placebo effect", and, more recently10, that: " (in this review) there was no condition which responds convincingly better to homeopathic treatment than to placebo ".
In 1991, Kleijnen et al. published a meta-analysis51 that reported a small positive effect for homeopathic treatments. However, the authors emphasized the fact that clear conclusions were not feasible, since most studies had a very low methodological quality, which could severely bias the results. Similarly, Linde et al.49, in a 1997 review of more than 100 studies, identified a favorable result for homeopathy. Also in this case, however, the authors admitted that publication bias could introduce an effect that was "difficult to estimate". In addition, they stated that: " we found little evidence of effectiveness of any single homeopathic approach on any single clinical condition".
The magnitude of the bias and the quality of the studies in these reviews is illustrated by a positive study that, when re-assessed, actually revealed no effect of homeopathy over placebo (the original analysis used an inadequate statistical methodology). However, the inadequate study was still accepted in these reviews and considered to be of good quality53,54. Similarly, the French government commissioned a high-quality trial with the objective of confirming (or not) 2 preliminary positive homeopathic studies of poor quality. This new trial did not yield any positive results, but all 3 studies were included in the reviews55.
Moreover, the presence of bias was later confirmed56-58, by means of a visual test that allowed for the identification of a publication bias in the "positive studies" direction. Another approach was the reproduction of the analysis of Linde et al., but taking into account only those studies judged to be of the highest quality59. The conclusion of this new analysis was that: " homeopathic remedies are associated with the same clinical effect as placebo" and that: " applying strict criteria, a meta-analysis of dilute homeopathic remedies shows no effect over placebo whatsoever" 59.
Also, in a re-assessment of their work60,61, the main authors of the 1997 review stated that: " there was clear evidence that [homeopathic] studies with better methodological quality tended to yield less positive results", and that: "The evidence of bias weakens the findings of our original meta-analysis It seems, therefore, likely that our meta-analysis at least overestimated the effects of homeopathic treatments".
Finally, 2 other recent reviews11,62 concluded that no effect can be detected when studies of better methodological quality are considered, and still another, specifically dealing with the post-operative ileus condition63, found negative results for the studies in which homeopathic preparations had been highly diluted.
As can be seen, the relationship between the lack of quality in a homeopathic study and its positive results arises in numerous studies, and some authors repeatedly warn against the fact that "alternative and complementary" medical journals create a serious publication bias in the field64. Thus, the message that consistently emerges is that homeopathic studies with positive results are those of low methodological quality, in which double-blind characteristics could not be assured, that had statistical methodological flaws or that were published in less rigorous journals54-67. For instance, in the study mentioned by Linde et al., only 20% of the trials could actually be considered as "blind"67.
In addition, other reasons for positive results can be noted:
- homeopathic preparations with a relatively low dilution may still retain pharmacological properties68. Even if these effects were not specific for the problem being addressed, the masking of the "intervention" group could be thus compromised.
- There are documented cases in which homeopathic medications had been adulterated69-71. For instance, Kerr and Saryan69, in an analysis of homeopathic preparations in the United States, found arsenic in 2 out of 6 tested samples. Also, in a recent review 120 samples of "alternative medicine" medications (including homeopathy), Gupta et al.71, in India, found that 38% of them were contaminated with corticosteroids. An older study in the United States70 detected a similar proportion.
Another homeopathic research topic that has yielded fully negative results is the one of "homeopathic provings"37-41. This is a fundamental procedure for the definition of homeopathic prescriptions. In these provings, arbitrary substances are given to a small number of healthy volunteers, and the subsequent symptoms are recorded by the volunteers. As mentioned, homeopathic prescriptions are then developed from the assumptions that these substances, ultra-diluted, could cure these same symptoms in sick subjects. For instance, in a recent experiment that tried to submit these assumptions to a double-blind test38, 47 volunteers were divided in 2 groups, one receiving a placebo, and the other, ultra-diluted belladonna. It was not possible to observe any discernible pattern in the symptoms reported by the 2 groups. Another interesting older example concerns an experiment on homeopathic Pulsatilla40 in which subjects received either a placebo or the homeopathic preparation in a cross-over design. No differences could be detected between the 2 groups, and an interesting result was that during the first month, clear symptoms were reported by all patients, probably due to the "novelty" characteristic of the study.
Physical foundations and in vitro research
The modern in vitro homeopathic research can be said to begin with the work of the French immunologist Jacques Benveniste, during the 1980s. In 1988, this researcher reported that in his laboratory, conclusive proof of homeopathic effects had been identified. According to him, water could retain a "memory" of its solutes and, with this memory, cause the degranulation of basophil cells in vitro, even after the solute had been diluted to 10-120.
Benveniste´s study72 was accepted for publication in the Nature journal, as long as a committee, assigned by the journal, could make an in loco replication attempt. This committee, however, concluded that73:
- the experiments were not double-blind;
- they "worked better" when performed by a specific member of Benveniste´s team;
- when the experiments did not work, they were discarded;
- no adequate statistical control methods were employed;
- when replicated with better controls, no effect could be detected.
In summary, " an extensive series of experiments which are statistically ill-controlled, from which no substantial effort has been made to exclude systematic error, including observer bias, and whose interpretation has been clouded by the exclusion of measures in conflict with the claim ".
Recently, an allegedly positive replication of this study was reported74. This new experiment was performed in 4 European laboratories, and a member of Benveniste´s original team took part in them. This study, however, was only published as a short, non-peer reviewed article, and, apparently, had methodological flaws that could compromise its results. For instance, the authors reported that ¼ of their data had to be discarded, although no clear justification was presented for that. Also, the statistical techniques used appear inadequate. For instance, consider the fractions of control x treatment degranulation reported in the study for "laboratory 1":
Using standard statistical formulae for a 2-proportions t-test75, one finds a 95% Confidence Interval of:
95% CI lab 1: [-0.03; 0.21] ; not statistically significant.
Finally, at least 5 other research groups tried to replicate Benveniste´s results, not one of them finding a positive effect76-80.
More recently, Benveniste stated that the "homeopathic potentialization" of a substance could be sent and recovered through electromagnetic means (for example, through an e-mail message)81. The "water memory" thus digitized could then be used to transfer the properties of the original solute to other substances, at long distance. More information on this (still non-substantiated) claim can be found in <http://www.digibio.com>.
Currently, research in this area still continues, basically coordinated by an international consortium named GIRI (International Research Group on Very Low Dose and High Dilution Effects, in French). The member list of this group has more than 150 scientists from many countries, and renowned members include the French immunologist Madeleine Bastide. In one of the few studies of the group that appeared in the scientific literature82,83, the authors attempted to study the effects of ultra-diluted thyroxine (10-60) in the growth of juvenile frogs (see Endler84 for more details). The authors report on the results of 4 experiments. In the first one, a very highly diluted thyroxine solution was applied to a tank in which juvenile frogs were developing. In the second, this solution was placed inside a sealed vial, which was then placed close to the tank (without contact with the water). In 2 other experiments, a procedure was used to "digitize" the properties of the solution and then to "communicate" them to water in another vial. Basically, in the 2 last experiments, a vial with the thyroxine preparation was connected to a coil wire and to an amplifier. An electric current was then applied to the wire, and "after filtering and noise reduction", the homeopathic effect was, allegedly, transferred to a pure water vial, also connected to the amplifier. In each of the 4 experiments, "diluted water (water 10-60)" was used as control. Results were similar in all the 4 experiments: frog growth was altered whether they were exposed to the ultra-diluted thyroxine, to the closely placed sealed vial, or to the vials to which the "digitized" properties had been transferred. The authors also reported that many other factors, for instance, the time of the year when the experiments were performed, appeared to influence the results.
From these experiments, the authors concluded that the homeopathic effect propagates through electromagnetic waves, which had transferred the homeopathic effect to the frogs. However, the most probable explanation is that the complex and non-blind characteristic of the work allowed for the introduction of bias, which would explain the altered rate of growth in all 4 experiments85,86.
Other GIRI members further expanded the implications of these results. For instance, the Belgian researcher William Betz reports that, in a similar situation, when both a placebo and a homeopathic preparation produced the same effect, Madeleine Bastide concluded that the placebo had been "contaminated" by the "homeopathic radiation", arising from the closely stored homeopathic preparation85.
Finally, Vickers87 performed a comprehensive review of the literature concerning "pre-clinical research" in homeopathy, concluding that: "There is a lack of independent replication of any pre-clinical research in homoeopathy. In the few instances where a research team has set out to replicate the work of another, either the results were negative or the methodology was questionable" .
In summary, similarly to the analysis concerning clinical trials, here, also, it is possible to identify a clear relationship between the low methodological quality of a homeopathic study and its positive results.
Still concerning the physical mechanisms of homeopathy, it is not possible to identify differences between homeopathic preparations and pure water by means of a nuclear magnetic ressonance (NMR) analysis88. It is well known that homeopathy "violates" the Avogadro number, that is, in a homeopathic preparation above a 10-24 dilution, not 1 molecule of the original solute is expected to be found. However, explanations for the putative action mechanisms for homeopathy under these circumstances are only very rarely presented. Worse yet, when examined, these few explanations turn out to be absolutely non-substantiated and non-scientific43,86. For instance, in a recent introduction to homeopathy in the British Medical Journal89, the authors made the (non-referenced) statement that "The complex lattice formations created by water molecules are thought by some to hold the key to homeopathy". The reason for such an oblique, unspecific citation must be the long history of non-corroborated search for such water structures, of which a recent example is Lo et al.90-92.
It should also be noticed that this low concern with a possible mechanism of action leaves unanswered arguments such as93: "It is estimated that in 60 seconds a person standing still generates about 100 000 particles skin fragments, salt, oil droplets, humidity and cosmetics which are large enough to damage an integrated circuit during its manufacturing process. Even if homeopathic medications were prepared in a room of maximum cleanness, the chance of one of these particles ending in the final preparation would be large. How could the water tell between what is the active substance and what is a sulphur molecule from a hairspray? How can it tell, in its memory, the molecule of the rotten liver of a duck among millions of organic particles that are thrown over it by the simple act of breathing?"
The statement that homeopathy is "discriminated by an "official science" cannot be supported by the results of the present review. The quantity and quality of the current research in this area indicates that, actually, the rejection of homeopathy as a valid scientific endeavor comes from the fact that the more recent research has thoroughly disconfirmed the main homeopathic hypotheses. Another conclusion concerns the relationship between the quality of a homeopathic study and its positive results. This is a clearly recognized phenomenon, which, for instance, admittedly "contaminated" the meta-analyses conducted in the 1990s that detected a (weak) favorable effect for homeopathic preparations.
In summary, the present review indicates that the weight of the modern evidence clearly disconfirms the hypothesis that ultra-diluted substances could have a noticeable clinical or pharmacological effect in living organisms.
My thanks to Drs. Wim Betz (Vrije Universitei, Belgium) and Wallace I Sampson, (Stanford University Medical School), for the discussion of some of the aspects of the present work, and for their help in identifying relevant references.
1. Rastogi DP, Singh VP, Singh V, et al. Homeopathy in HIV infection: a trial report of double-blind placebo controlled study. Br Homeopath J 1999; 88(2):49-57. [ Links ]
3. Hill C, Doyon F. Review of randomized trials of homeopathy. Rev Epidemiol Santé Pub 1990; 38:39-47. [ Links ]
4. Kurz R. Clinical medicine versus homeopathy. Padiatric Padology 1992; 27(2):37-41. [ Links ]
5. Aulas J. Homeopathy update. Préscrire Int 1996; 15(155):674-84. [ Links ]
6. Linde K, Melchart D. Randomized controlled trials of individualized homeopathy: a state-of-the-art review. J Altern Complement Med 1998 Winter; 4(4):371-88. [ Links ]
7. Ernst E, Pittler MH. Efficacy of homeopathic arnica: a systematic review of placebo-controlled clinical trials. Arch Surg 1998; 13(11):1187-90. [ Links ]
8. Ernst E. Homeopathic prophylaxis of headaches and migraine? A systematic review. J Pain Symptom Management 1999; 18(5):353-7. [ Links ]
9. Stevinson C, Ernst E. Complementary/alternative therapies for premenstrual syndrome: a systematic review of randomized controlled trials. Am J Obstet Gynecol 2001 Jul; 185(1): 227-35. [ Links ]
10. Ernst E. A systematic review of systematic reviews of homeopathy. Br J Clin Pharmacol 2002; 54(6): 577-582. [ Links ]
11. CRD. Homeopathy. Effective Health Care 2002; 7(3) [Centre for Reviews and Dissemination], University of York, York, UK. [ Links ]
12. De Lange ESM, Blommers J, Kuik DJ, et al. Effect of homeopathic medicines on daily burden of symptoms in children with recurrent upper respiratory tract infections. Br Med J 1994; 309(6965):1329-32. [ Links ]
13. Lewith GT, Watkins AD, Hyland ME, et al. Use of ultramolecular potencies of allergen to treat asthmatic people allergic to house dust mite: double blind randomised controlled clinical trial. Br Med J 2002; 324(7336):520. [ Links ]
14. Fingerhut A. Homeopathy for the restoration of transit after abdominal surgery. Chirurgie 1990; 116 404-8. [ Links ]
15. White A, Slade P, Hunt C, et al. Individualised homeopathy as an adjunct in the treatment of childhood asthma: a randomised placebo controlled trial. Thorax 2003; 58(4): 317-321. [ Links ]
16. Labrecque M, Audet D, Latulippe LG, et al. Homeopathic treatment of plantar warts. Can Med Assoc J 1992; 146:1749-1753. [ Links ]
17. Kainz JT, Kozel G, Haidvogl M et al. Homeopathic versus placebo therapy of children with warts on the hands: a randomized double-blind clinical trial Dermatol 1996; 193(4): 318-20. [ Links ]
18. Smolle J, Prause G, Kerl H. A double-blind, controlled clinical trial of homeopathy and analysis of lunar phases and postoperative outcome. Arch Dermatol 1998 Nov; 134(11):1368-70. [ Links ]
19. Lokken P, Straumsheim PA, Tveiten D, et al. Effect of homoeopathy on pain and other events after acute trauma: placebo controlled trial with bilateral oral surgery. Br Med J 1995; 310(6992): 1439-42. [ Links ]
20. Hart O, Mullee MA, Lewith G, et al. Double-blind, placebo controlled randomized clinical trial of homoeopathic arnica C30 for pain and infection after total abdominal hysterectomy. J Royal Soc Med 1997; 90(2):73-8. [ Links ]
21. Vickers AJ, Fisher P, Smith C, et al. Homeopathy for delayed onset of muscle soreness: a randomised double blind placebo controlled trial. Br J Sports Med 1997; 31:304-7. [ Links ]
22. Vickers AJ, Fisher P, Smith C, et al. Homeopathic Arnica 30x is ineffective for muscle soreness after long-distance running: a randomized, double-blind, placebo-controlled trial. Clin J Pain 1998; 14(3): 227-31. [ Links ]
23. Shealy CN, Thomlinson RP, Borgmeyer V. Osteoarthritic pain: a comparison of homeopathy and acetaminophen. Am J Pain Management 1998; 8:89-91. [ Links ]
24. Friese K, Feucher U, Moeller H. Homeopathic management of adenoid vegetations, results of a prospective, randomized double-blind study. HNO 1997; 45:618-624. [ Links ]
25. Whitmarsh T, Coleston Shields D, Steiner T. Double-blind randomized placebo controlled study of homeopathic treatment of chronic headaches. Cephalgia 1997; 17: 600-4. [ Links ]
26. Walach H, Haeusler W, Lowes T, et al. Classical homeopathic treatment of chronic headaches. Cephalgia 1997; 17(2):119-26. [ Links ]
27. Walach H, Lowes T, Mussbach D, et al. The long-term effects of homeopathic treatment of chronic headaches: 1 year follow up. Cephalgia 2000; 20(9):835-7. [ Links ]
28. Straumsheim P, Borchgrevink C, Mowinckel P, et al. Homeopathic treatment of migraine: a double blind, placebo controlled trial of 68 patients. Br Homeopath J 2000 Jan; 89(1):4-7. [ Links ]
29. Simpson JJ, Donaldson ID WE. Use of homeopathy in the treatment of tinnitus. Br J Audiol 1998; 32(4):227-33. [ Links ]
30. Harrison H, Fixsen A, Vickers A. A randomized comparison of homeopathic and standard care for the treatment of glue ear in children. Complement Ther Med 1999; 7(3):132-5. [ Links ]
31. Hoare C, Li WPA, Williams H - Systematic review of treatments for atopic eczema. Health Technol Assess 2000; 4(37):1-191. [ Links ]
32. Andrade LE, Ferraz MB, Atra E, et al. A randomized controlled trial to evaluate the effectiveness of homeopathy in rheumatoid arthritis Scand J Rheumatol 1991; 20(3):204-208. [ Links ]
33. Fisher P, Scott DL - A randomized controlled trial of homeopathy in rheumatoid arthritis. Rheumatol 2001 Sept; 40(9):1052-1055. [ Links ]
34. Ramelet AA, Buchheim G, Lorenz P, et al. Homeopathic Arnica in postoperative haematomas: a double-blind study. Dermatol 2000; 201(4): 347-8. [ Links ]
35. Stevinson C, Devaraj VS, Fountain-Barber A, et al. Homeopathic arnica for prevention of pain and bruising: randomised placebo-controlled trial in hand surgery. J R Soc Med 2003; 96(2): 60-65. [ Links ]
36. Bonne O, Shemer Y, Gorali Y, et al. A randomized, double-blind, placebo-controlled study of classical homeopathy in generalized anxiety disorder. J Clin Psychiatry 2003; 64(3): 282-287. [ Links ]
37. Wallach H. Does a highly diluted homeopathic drug act as a placebo in health volunteers? J Psychosom Res 1993; 37(8): 851-60. [ Links ]
38. Walach H, Koster H, Hennig T, et al. The effects of homeopathic Belladonna 30CH in healthy volunteers: a randomized double-blind experiment. J Psychosom Res 2001; 50(3):155-60. [ Links ]
39. Goodyear K, Lewith G, Low JL. Randomized double-blind placebo-controlled trial of homoeopathic `proving' for Belladonna C30. J R Soc Med 1998 Nov; 91(11):579-82. [ Links ]
40. Campbell A. Homeopathy in perspective myth and reality [Chapter11] Available <www.acampbell.org.uk/%20freethinker/altmed/homeomythology> June 2003). [ Links ]
41. Vickers AJ, Van Haselen R, Heger M. Can homeopathically prepared mercury cause symptoms in healthy volunteers? J Altern Complement Med 2001 Apr; 7(2):141-8. [ Links ]
42. Grabia S, Ernst E. Homeopathic aggravations: a systematic review of randomised, placebo-controlled clinical trials. Homeopathy 2003; 92(2):92-8. [ Links ]
43. Ramey D, Wagner M, Imrie RH, et al. Homeopathy and science: a closer look. Available <http://spot.colorado.edu/~vstenger/Medicine/Homeop.html> - June 2003. [ Links ]
44. Jacobs J, Jimenez LM, Gloyd SS et al. Treatment of acute childhood diarrhea with homeopathic medicine: a randomized clinical trial in Nicaragua. Pediatrics 1994; 93(5):719-25. [ Links ]
45. Taylor MA, Reilly D, Lewellyn-Jones RH, et al. Randomised controlled trial of homoeopathy versus placebo in perennial allergic rhinitis with overview of four trial series. Br Med J 2000; Aug 19-26; 321(7259):471-6. [ Links ]
46. ¾ Clinical updates: Homeopathy - Physician's Weekly 1997; XIV(13), (available <http://www.physweekly.com/archive/97/04_07_97/cu5.html> - June 2003). [ Links ]
47. Sampson W, London W. Analysis of homeopathic treatment of childhood diarrhea. Pediatrics 1995; 96(5): 961-64. [ Links ]
48. Raso J. Homeopathy: if `less is more,' is nothing best? Priorities for Health 2000; 12(3) (available <www.acsh.org/publications/priorities/1203/homeopathy.html> - June 2003). [ Links ]
49. Linde K, Clausius N, Ramirez G, et al. Are the clinical effects of homoeopathy placebo effects? A meta-analysis of placebo-controlled trials. Lancet 1997; 350:834-43. [ Links ]
50. Linde K, Jonas WB. Meta-analysis of homoeopathy trials. Lancet 1998; 351:368. [ Links ]
51. Kleijnen J, Knipschild P, Ter Riet G. Clinical trials of homoeopathy. Br Med J 1991; Feb 9; 302(6772): 316-23. [ Links ]
52. Fisher P, Greenwood A, Huskinnsson E, et al. Effect of homoeopathic treatment on fibrositis Br Med J 1989; 299:365-6. [ Links ]
53. Coulquhoun D. Re-analysis of clinical trial of homoeopathic treatment of fibrositis. Lancet 1990; 336: 441-2. [ Links ]
54. Wall PD. Trials of homoeopathy. Br Med J 1991; 302(2):529. [ Links ]
55. Kahn MF. Meta-analysis of homoeopathy trials. Lancet 1998; 351:365. [ Links ]
56. Egger M, Davey Smith G, Schneider M et al. Bias in meta-analysis detected by a simple, graphical test. Br Med J 1997; 315(7109):629-34. [ Links ]
57. Egger M, Smith GD, Head CM. Bias in meta-analysis detected by a simple, graphical test. Br Med J 1998; 316(7129):471. [ Links ]
58. Sterne JAC, Egger M, Smith GD. Systematic reviews in health care: investigating and dealing with publication and other biases in meta-analysis. Br Med J 2001; 323:101-105. [ Links ]
59. Ernst E. Are highly dilute homeopathic remedies placebos? Perfusion 1998; 11: 291-292. [ Links ]
60. Linde K, Scholz M, Ramirez G, et al. Impact of study quality on outcome in placebo-controlled trials of homeopathy. J Clin Epidemiol 1999; 52(7):631-6. [ Links ]
61. Ernst E, Pittler MH. Re-analysis of previous meta-analysis of clinical trials of homeopathy. J Clin Epidemiol 2000 Nov; 53(11):1188. [ Links ]
62. Cucherat M, Haugh MC, Gooch M, et al. Evidence of clinical efficacy of homeopathy. A meta-analysis of clinical trials. Eur J Clin Pharmacol 2000; 56(1):27-33. [ Links ]
63. Barnes J, Resch KL, Ernst E. Homeopathy for post-operative ileus? A meta-analysis. J Clin Gastroentherol 1997; 25(4): 628-33. [ Links ]
64. Ernst E, Pittler MH. Alternative Therapy Bias. Nature 1997; 365:480. [ Links ]
65. Pittler MH, Abbot NC, Harkness EF, et al. Location bias in controlled clinical trials of complementary/alternative therapies. J Clin Epidemiol 2000; 53(5): 485-9. [ Links ]
66. Linde K, Jonas WB, Melchart D, et al. The methodological quality of randomized controlled trials of homeopathy, herbal medicines and acupuncture. Int J Epidemiol 2001 Jun; 30(3):526-531. [ Links ]
67. Langman MJS. Homeopathy trials: reason for good ones but are they warranted? Lancet 1997; 350(20): 825. [ Links ]
68. Wagner MW. Is homeopathy "new science" or "new age"? The Scientific Review of Alternative Medicine 1997; 1(1): 7-12. [ Links ]
69. Kerr H, Saryan L. Arsenic content of homeopathic medicines. J Toxicol Clin Toxicol 1986; 24(5): 451-9. [ Links ]
70. Morice A. Adulterated homeopathic cure for asthma. Lancet 1986; 12(1):8374. [ Links ]
71. Gupta SK, Kaleekal T, Joshi S. Misuse of corticosteroids in some of the drugs dispensed as preparations from alternative systems of medicine in India. Pharmacoepidemiol Drug Saf 2000 Dec; 9(7):599-602. [ Links ]
72. Davenas E, Beauvais F, Amara J, et al. Human basophil degranulation triggered by very dilute antiserum against IgE. Nature 1988; 333(6176):816-818. [ Links ]
73. Madox J, Randi J, Stewart WW. High dilution experiments a delusion. Nature 1988; 334(6180): 287-291. [ Links ]
74. Belon P, Cumps J, Ennis M, et al. Inhibition of human basophil degranulation by successive histamine dilutions: results of a European multi-centre trial. Inflamm Res 1999 Apr; 48 Suppl 1:S17-8. [ Links ]
75. Wonnacott RJ, Wonnacott TH. Introductory Statistics (Fourth Edition), p. 242 Wiley, New York 1985. [ Links ]
76. Seagrave JC. Evidence of non-reproducibility. Nature 1988; 334:559. [ Links ]
77. Bonini S, Adriani E, Balsano F. Evidence of non-reproducibility. Nature 1988; 334:559. [ Links ]
78. Metzger H, Dreskin SC. Only the smile is left. Nature 1988; 334:375. [ Links ]
79. Ovelgonne JH, Bol AWJM, Hop WCJ, et al. Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties. Experientia 1992; 48:504-8. [ Links ]
80. Hirst SJ, Hayes MA, Burridge J, et al. Human basophil degranulation is not triggered by very dilute antiserum against IgE. Nature 1993; 366(6455):525-7. [ Links ]
81. Benveniste J, Jurgens P, Hsueh P, et al. Transatlantic transfer of digitized antigen signal by telephone link J Allergy Clin Immun AAAAI/AAI/CIS Joint Meeting 1997; 21-6. [ Links ]
82. Endler PC, Pongratz W, Kastberger G, et al. The effect of high diluted agitated thyroxine on the climbing activity of frogs. Vet Hum Toxicol 1994; 36(1):56-59. [ Links ]
83. Endler PC, Pongratz W, Smith CW, et al. - Non-molecular information transfer from thyroxine to frogs with regard to homeopathic toxicology. Vet Hum Toxicol 1995; 37(3):259-60. [ Links ]
84. Endler PC, Pongratz W, Smith CW, et al. Non-Molecular information transfer from thyroxine to frogs by means of homoeopathic preparation and electronics (available <http://www.giriweb.com/endler.htm> - June 2003). [ Links ]
85. Betz W - Homeopathy logic and tactics. First World Skeptics Congress Buffalo, New York, 20-23 1996 June. [ Links ]
86. Park RL - Alternative medicine and the laws of physics. Skeptical Inquirer 1997; 21(5): 24-28. [ Links ]
87. Vickers AJ - Independent replication of pre-clinical research in homeopathy: a systematic review. Forsch Komplementarmed 1999 Dec; 6(6):311-20. [ Links ]
88. Milgrom LR, King KR, Lee J, et al. On the investigation of homeopathic potencies using low resolution NMR T2 relaxation times: an experimental and critical survey of the work of Roland Conte et al. Br Homeopath J 2001 Jan; 90(1):5-13. [ Links ]
89 Vickers A, Zollman C - ABC of complementary medicine: Homoeopathy Br Med J 1999; 319:1115-8. [ Links ]
90. Lo S-Y, Lo A, Chong LW, et al. Physical properties of water with IE structures. Mod Phys Letters B 1996; 10(19):921-930. [ Links ]
91. State of Oregon DOJ. Affidavit of Dr. Paul Engelking. State of Oregon Department of Justice, Financial Fraud/Consumer Protection, Circuit Court of Oregon, Marion County, 1997. (available <www.doj.state.or.us/FinFraud/engel-king.htm> - June 2003). [ Links ]
92. State of Oregon DOJ. Affidavit of Andrew W Blackwood. State of Oregon Department of Justice, Financial Fraud/Consumer Protection, Circuit Court of Oregon Marin County, 1997 (available <http://www.doj.state.or.us/FinFraud/blueblaff.htm> - June 2003). [ Links ]
93. Colluci Jr. J. O físico e a pororoca Observatório da Imprensa, 14/02/2001 Edition (available <www.observatoriodaimprensa.com.br/ofjor/ofc140220013.htm> - June 2003). [ Links ]
Received for publication on October 7, 2002.