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Print version ISSN 0066-782X
Arq. Bras. Cardiol. vol.88 no.4 São Paulo Apr. 2007
Flávia Aparecida de OliveiraI, III; Vicente de Paula Antunes TeixeiraII, III; Ruy de Souza Lino JuniorI; Marina Clare VinaudI; Marlene Antônia dos ReisII, III
IGeneral Pathology Sector, Tropical
Pathology and Public Health Institute (IPTSP), Federal University of Goiás
IIGeneral Pathology Sector, Federal University of Triângulo Mineiro (UFTM)
IIIPathology Post-graduation Programme, UFTM, Goiânia-GO, Uberaba, MG, Brazil
To describe the macroscopic characteristics of chronic Chagas heart disease
in autopsied elderly.
METHODS: The elderly studied were 60 or older. Twenty of them had chronic Chagas heart disease (CHD) and positive serology for the disease, and 14 had no heart disease (WHD) nor morphological changes suggestive of it and were serologically negative for Chagas disease.
RESULTS: The CHD elderly had cardiac weight greater than the WHD (385 ± 141.1 vs 306.8 ± 62.1g, respectively; p > 0.05), in addition to significantly higher heart weight-to-body weight ratio (0.71% [0.5-1.42%] vs 0.59% [0.47-0.91%] p < 0.05). When compared, the CHD elderly presented lower fibrous thickening and/or atherosclerosis in the ascending aorta, mitral and tricuspid valves, and left and right coronaries than the WHD elderly. In the aortic and mitral valves, the lesions were significantly less severe (p < 0.05). Left ventricular apical lesion was observed in 45% of the CHD elderly, and intracardiac thrombosis in the left ventricle was found in 10% of them.
CONCLUSION: Fibrous thickening and/or atherosclerosis were found to be less severe in the valves and arteries of the CHD elderly. Moreover, heart weight and intracardiac thrombosis frequency were lower than those detailed in the literature for non-elderly individuals.
Key words: Chagas cardimiopathy; atherosclerosis; Chagas disease; aging.
According to the World Health Organization (WHO), thirteen million individuals are infected by the protozoan Trypanosoma cruzi. Around three million cases are symptomatic, and the annual incidence of new cases is one hundred thousand to two hundred thousand1. In Brazil, there is an association of epidemiological and demographic transitions2. Among the various chronic illnesses which afflict the elderly, Chagas disease in its chronic phase is found in endemic areas. Infection by T. cruzi in the elderly represents a public health problem due to reduction in preponderance and interruption in transmission of Chagas disease, which determines an increase in aging infected individuals3. Although chronic Chagas heart disease is the most serious lesion caused by Chagas disease, the disease is still little understood. Most infected individuals remain asymptomatic, and around 30% present cardiac and/or digestive complications in the late phase of the disease4.
In chronic Chagas heart disease described in non-elderly individuals, the macroscopic changes vary from an apparently normal heart with dilation of the right auricle to an increase in weight, global dilation associated with hypertrophy, subendocardial fibrosis at the tip of the ventricle and papillary muscles, endocardial thickening, left ventricular apical lesion with or without intracardiac thrombosis, intracardiac thrombosis, extreme cardiomegaly with the heart weighing over 1000g, and changes in the aorta, such as supravalvular dilation and aneurysm formation5-7.
The macroscopic aspects of chronic Chagas heart disease in aging were not found in the literature. In the few studies investigating the disease in the elderly, most refer to clinical aspects8-12. Therefore, based on the increase in aging individuals with Chagas disease and on the fact that there are few pathological studies of Chagas disease in the elderly, the aim of this study was to describe the macroscopic changes of chronic Chagas heart disease in autopsied elderly.
The elderly, whether with chronic Chagas disease or not, were selected from the reports on autopsies performed at the Teaching Hospital of the Federal University of Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, from 1970 to 2000. The elderly selected were age 60 or older, and their demographic data are shown in Table 1. Among these data, causes of death were grouped into cardiovascular, infectious, neoplastic, digestive, and others, according to the description in the autopsy report of the process that could have determined the ultimate cause of death13.
Two groups were formed for the macroscopic analysis of the hearts: one consisting of fourteen elderly individuals without heart disease (WHD elderly) and the other consisting of 20 elderly with chronic Chagas heart disease (CHD elderly). In the CHD group, cases of emphysema; bronchitis; ischemic, hypertensive, and rheumatic heart diseases; and/or cor pulmonale were excluded, according to the macroscopic and microscopic morphologic characteristics. Cases with positive serology for Chagas disease, as well as those with morphologic characteristics suggestive of Chagas heart disease, were included in this group14. In the WHD group, Chagas heart disease cases and those with positive serology for Chagas disease were excluded, as well as the lesions excluded in the CHD elderly group15.
The following macroscopic characteristics were evaluated: fibrous thickening and atherosclerosis in the ascending aorta, in the pulmonary trunk, and in the aortic, pulmonary, mitral, and tricuspid valves; epicarditis; the shape of the heart; endocardial fibroelastosis; left ventricular apical lesion; and endocardial thrombosis16. The severity of the processes was classified semiquantitatively according to predominance in the structure analyzed as follows: absent, mild when up to 25% of the structure was affected, moderate when 26% to 50% of the structure was affected, and severe when over 51% of the structure was affected. Cardiac weight was obtained from the autopsy reports, and the heart weight-to-body weight ratio (HW/BW) was calculated with the weight in grams multiplied by 100. The HW/BW normality pattern was considered as less than or equal to 0.5%17.
Statistical analysis was performed using the SigmaStat 2.03 program. Comparison of two groups with normal distribution and homogenous variance was performed using the Student's t-test and, when this was not the case, the Mann Whitney test to compare two groups, or the Kruskal-Wallis, followed by the Dunn test, to compare more than two groups. The significance level was set at 5% (p<0.05). This research project was approved by the Ethics Committee of the UFTM, file No. 357.
The cardiac weight of the CHD elderly was greater than that of the WHD elderly (385 ± 141.1 vs 306.8 ± 62.1g, respectively; p>0.05), and their HW/BW ratio was significantly higher than that of the CHD elderly (0.71% [0.5-1.42%] vs. 0.59% [0.47-0.91%], p < 0.05. The CHD elderly, when compared to the WHD elderly, presented less fibrous thickening and/or atherosclerosis in the ascending aorta segment (Figure 1), mitral and tricuspid valves, and left and right coronaries (Figure 2), but the difference was not statistically significant (p>0.05). On the other hand, atherosclerosis in the aortic valve (Figure 1) and fibrous thickening in the mitral valve (Figure 3) were significantly less severe in the CHD elderly (p< 0.05) (Table 2). Global cardiac dilation, however, was significantly more severe in the CHD elderly (p < 0.05) (Table 2). All other macroscopic changes are shown in Table 2.
In the CHD elderly, left ventricular apical lesion was detected in 45% (n = 9) and intracardiac thrombosis in the left ventricle in 10% (n=2), all of which were associated with left ventricular apical lesion. In these cases of intracardiac thrombosis, cardiac weight was 520 g and 850 g. Cardiac weight of the CHD elderly with left ventricular apical lesion was greater than in those without the lesion (431.1 ± 186.6 vs 347.3 ± 80.4g, respectively; p > 0.05).
According to a literary review, this appears to be the first description of macroscopic characteristics of chronic Chagas heart disease in aging. In general, in the macroscopic cardiac evaluation, the CHD elderly presented less severe fibrous thickening and/or atherosclerosis in the valves and arteries evaluated than the WHD elderly. Moreover, cardiac weight and intracardiac thrombosis frequency were lower than that described in the literature for non-elderly individuals18-20.
Atherosclerosis in the aortic valve and fibrous thickening in the mitral valve were significantly less severe in CHD elderly than in the WHD. In another study no difference was noted in the frequency of myocardial infarction and coronary atherosclerosis in non-elderly individuals with or without chronic Chagas disease. Morphological findings of the artery disease were also similar in both groups21. Modulation in inflammatory response probably occurs22 in chronic Chagas heart disease in the elderly, which could lead to the development of less severe atherosclerotic lesions. Therefore, it is possible that inflammatory modulation in Chagas heart disease enables the modulation of common pathways for the development of heart disease and atherosclerosis, which would indirectly inhibit the progression of the atherosclerotic lesions.
Global heart dilation was significantly more frequent in CHD elderly; severe chronic epicarditis and endocardial fibroelastosis were also predominant in the CHD elderly, yet there was no statistical difference in proportions. Cardiac dilation in Chagas heart disease found in the elderly is a characteristic of the disease, as already described in the non-elderly16,23. On the other hand, the other macroscopic changes described previously were also found in the WHD elderly. Yet, although these changes were not specific, they increased in intensity in Chagas heart disease. Ventricular dilation and reduction in myocardial compliance are also related to the destruction of cardiomyocytes associated with fibrosis24. This fact may have contributed to the cardiac dilation found in the CHD elderly.
Left ventricular apical lesion and intracardiac thrombosis in the left ventricle associated with apical lesion were found only in the CHD elderly. This fact is in accordance with the literature, which describes left ventricular apical lesion as a lesion typical of Chagas heart disease and is considered one of the most important macroscopic changes for the diagnosis of the heart disease, which in turn can lead to intracardiac thrombus formation25.
Left ventricular apical lesion was found in 45% of CHD elderly cases and intracardiac thrombosis in 10% of the cases, all of which were associated with left ventricular apical lesion. These frequencies were lower than in another study which describes left ventricular apical lesion in 53.2% of the cases, in which 49.5% also presented cardiac thrombosis18. Other authors reported 73% of intracardiac thrombosis in severe Chagas heart disease in non-elderly individuals. Left ventricular apical lesion was described in 68% of the cases, 48% of which were significantly associated with apical thrombosis19. Moreover, in large left ventricular apical lesions, besides the risk of thrombosis, there is also the danger of arrhythmia and changes in heart function18.
The lesser frequency of left ventricular apical lesion and intracardiac thrombosis in CHD elderly shows that, in the elderly studied, Chagas heart disease is characterized by milder forms that allow functional adaptation of the heart to lesions in the organ, thus enabling survival. Cardiac weight was greater in the individuals that had left ventricular apical lesion and intracardiac thrombosis, probably due to the more severe myocardial lesions in these cases as described by other authors19.
Cardiac weight in the CHD elderly (385±141.1g) was lower than that found in literature, where the cardiac weight of non-elderly individuals varies from 415 ± 136.8 g to 568.49 ± 133.79 g17,20,23. Likewise, the median HW/BW ratio in the CHD elderly (0.71% [0.5-1.42%]) was less than the average found in the literature for non-elderly individuals (1.1 ± 0.22%)17. Cardiac weight in the CHD elderly lower than that described in the literature shows that although heart enlargement also occurs in the elderly, it seems to be less marked, probably due to less severe myocardial lesions during aging.
Macroscopic lesions in CHD elderly were qualitatively similar to those described in the literature for non-elderly individuals, but less severe. The study of chronic Chagas heart disease may also contribute toward a greater understanding of other lesions, such as atherosclerosis. These mechanisms may be involved in the modulations that produce the characteristics peculiar to Chagas heart disease in the elderly. Therefore, less severe fibrous thickening and/or atherosclerosis in the valves and arteries of CHD elderly were found. In addition, cardiac weight and intracardiac thrombosis frequency were lower than described in non-elderly individuals.
We would like to thank CAPES, FAPEMIG, FUNEPU, CNPq and Instituto de Patologia Tropical e Saúde Pública (IPTSP / UFG) for their financial support. We are also grateful to Aloísio Costa, Lourimar J. Morais, Maria Helena S.C. Batista, and Sônia M. Sobrinho for their technical support.
Potential Conflict of Interest
No potential conflict of interest relevant to this article was reported.
1. Morel CM, Lazdins J. Chagas disease. Nature Rev Microbiol. 2003; 1: 14-5. [ Links ]
2. Chaimowicz F. A saúde dos idosos brasileiros às vésperas do século XXI: problemas, projeções e alternativas. Rev Saúde Pública. 1997; 31: 184-200. [ Links ]
3. Lima e Costa MFF, Barreto SM, Guerra HL, Firmo JOA, Uchoa E, Vidigal PG. Ageing with Trypanosoma cruzi infection in a community where the transmission has been interrupted: the Bambuí Health and Ageing Study (BHAS). Int J Epidemiol. 2001; 30: 887-93. [ Links ]
4. Higuchi ML, Benvenuti LA, Reis MM, Metzger M. Pathophysiology of the heart in Chagas´ disease: current status and new developments. Cardiovasc Res. 2003; 60: 96-107. [ Links ]
5. Andrade Z, Andrade SG. A patogenia da miocardite crônica chagásica. Arq Bras Med. 1955; (7-8): 279-88. [ Links ]
6. Köberle F. Cardiopatia chagásica. Hospital. 1958; 53: 311-46. [ Links ]
7. Lopes ER, Chapadeiro E, Andrade ZA, Almeida HO, Rocha A. Anatomia patológica de corações de chagásicos assintomáticos falecidos de modo violento. Mem Inst Oswaldo Cruz. 1981; 76: 189-97. [ Links ]
8. Carneiro O, Rezende JM. Doença de Chagas e longevidade. Arq Bras Cardiol. 1982; 38: 381-4. [ Links ]
9. Carvalho ET Fº, Figueira JL, Pasini U, Forti NA, Curiati JAE, Ferreira MLM, Azul LGS. Aspectos da doença de Chagas no idoso. Arq Bras Cardiol. 1985; 45: 103-7. [ Links ]
10. Bestetti RB, Ramos CP, Godoy RA, Oliveira JS. Chronic Chagas' heart disease in the elderly: a clinicopathologic study. Cardiology. 1987; 74: 344-51. [ Links ]
11. Menezes M, Rocha A, Silva AC, Silva AM. Basic causes of death in elderly patients with Chagas' disease. Arq Bras Cardiol. 1989; 52: 75-8. [ Links ]
12. Resende LAPR, Carneiro ACF, Ferreira BDC, Silva RAG, Silva VJD, Prata A, et al. Análise temporal da variabilidade de freqüência cardíaca no estado basal em idosos chagásicos na forma indeterminada em área endêmica. Rev Soc Bras Med Trop. 2003; 36: 703-6. [ Links ]
13. Reis MA, Costa RS, Ferraz AS. Causes of death in renal transplant recipients: a study of 102 autopsies from 1968 to 1991. J R Soc Med. 1995; 88: 24-7. [ Links ]
14. Lopes ER, Chapadeiro E, Batista SM, Cunha JG, Rocha A, Miziara L, et al. Post-mortem diagnosis of chronic Chagas´s disease: comparative evaluation of three serological tests on pericardial fluid. Trans R Trop Med Hyg. 1978; 72: 244-6. [ Links ]
15. Kitzman DW, Scholz DG, Hagen PT, Ilstrup DM, Edwards WD. Age-related changes in normal human hearts during the first 10 decades of life. Part II (maturity): a quantitative anatomic study of 765 specimens from subjects 20 to 99 years old. Mayo Clin Proc. 1988; 63: 137-46. [ Links ]
16. Teixeira VPA, Gobbi H, Araújo WF, Almeida HO. Algumas alterações macroscópicas do coração de chagásicos crônicos com "megas" e sem "megas" com insuficiência cardíaca. Rev Goiana Med. 1984; 30: 43-8. [ Links ]
17. Almeida HO, Teixeira VPA, Araújo WF. Comportamento do peso do coração e do corpo em chagásicos crônicos com e sem "megas". Rev Soc Bras Med Trop. 1979; 13: 85-9. [ Links ]
18. Almeida HO. A "lesão vorticilar" da cardiopatia chagásica crônica: aspectos morfológicos. Rev Goiana Med. 1982; 28: 23-31. [ Links ]
19. Arteaga-Fernández E, Barreto ACP, Ianni BM, Mady C, Lopes EA, Vianna CB, et al. Trombose cardíaca e embolia em pacientes falecidos de cardiopatia chagásica crônica. Arq Bras Cardiol. 1989; 52: 189-91. [ Links ]
20. Tavares-Neto J. Correlação do peso do coração de chagásicos e controles, com algumas características anatomopatológicas. Rev Pat Trop. 1990; 19: 25-34. [ Links ]
21. Lopes ER, de Mesquita PM, de Mesquita LF, Chapadeiro E. Coronary arteriosclerosis and myocardial infarction in chronic Chagas´ disease. Arq Bras Cardiol. 1995; 65: 143-5. [ Links ]
22. Fan J, Watanabe T. Inflamatory reactions in the pathogenesis of atherosclerosis. J Atheroscler Thromb. 2003; 10: 63-71. [ Links ]
23. Lopes ER, Chapadeiro E, Almeida HO, Rocha A. Contribuição ao estudo da anatomia patológica dos corações de chagásicos falecidos subitamente. Rev Soc Bras Med Trop. 1975; 9: 262-82. [ Links ]
24. Higuchi ML, Fukasawa S, Brito T, Parzianello LC, Bellotti G, Ramires JAF. Different microcirculatory and interstitial matrix patterns in idiopathic dilated cardiomyopathy and Chagas´ disease: a three-dimensional confocal microscopy study. Heart. 1999; 82: 279-85. [ Links ]
25. Menezes H, Koberle F. Do valor do exame macroscópico no diagnóstico da cardiopatia chagásica. Hospital. 1965; 68: 140-4. [ Links ]
Flávia Aparecida de Oliveira
Disciplina de Patologia Geral.
Instituto de Patologia Tropical e Saúde Pública
Rua 235, s/n, QD 62, LT AR1, Setor Universitário
74605-050 Goiânia, GO - Brazil
Manuscript received July 28, 2006; revised manuscript received July 28, 2006; accepted October 5, 2006.