SciELO - Scientific Electronic Library Online

 
vol.92 issue5Relationship between electrocardiogram with diabetes mellitus and metabolic syndrome in Japanese-BraziliansDose-response curve to exercise in hypertensive individuals: analysis of the number of sessions to the hypotensive effect author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

Share


Arquivos Brasileiros de Cardiologia

Print version ISSN 0066-782X

Arq. Bras. Cardiol. vol.92 no.5 São Paulo May 2009

http://dx.doi.org/10.1590/S0066-782X2009000500009 

ORIGINAL ARTICLE

 

Effect of exercise training on aging-induced changes in rat papillary muscle

 

 

Laura Beatriz Mesiano MaifrinoI, IV; Rubens Correa AraújoI; Cristiane Castro FacciniIV; Edson Aparecido LibertiII; Eliane Florêncio GamaI; Antonio Augusto Coppi Maciel RibeiroIII; Romeu Rodrigues de SouzaI

ILaboratório de Biodinâmica, Universidade São Judas Tadeu; São Paulo, SP - Brazil
IIDepartamento de Anatomia, Instituto de Ciências Biomédicas; São Paulo, SP - Brazil
IIIInstituto de Ciências Biomédicas e Faculdade de Medicina Veterinária, Universidade de São Paulo; São Paulo, SP - Brazil
IVInstituto Dante Pazzanese de Cardiologia, São Paulo, SP - Brazil

Mailing address

 

 


SUMMARY

BACKGROUND: The effects of aging on papillary muscle have been widely demonstrated, but no data on the effects of exercise on the age-related changes are available.
OBJECTIVE: To analyze the effects of aging on the morphological and quantitative properties of papillary muscle and investigate whether a long-term moderate exercise program would exert a protective effect against the effects of aging.
METHODS: We used electron microscopy to study the density of myocytes, capillaries and connective tissue and the cross-sectional area of myocytes of the papillary muscle of the left ventricle of 6- and 13-month-old untrained and exercised Wistar rats.
RESULTS: As expected, the volume density of myocytes declined significantly (p<0.05) with aging. The length density of myocardial capillaries also declined with aging, but not significantly. The interstitial volume fraction of the papillary muscle tissue increased significantly (P<0.05) with age. The number of myocyte profiles showed a reduction of 20% that was accompanied by myocyte hypertrophy in the aged rats (P<0.05). Animals submitted to a 60-minute daily session,, 5 days/wk at 1.8 km.h-1 of moderate running on a treadmill for 28 weeks showed a reversion of all the observed aging effects on papillary muscle.
CONCLUSION: The present study supports the concept that long-term exercise training restrains the aging-related deleterious changes in the papillary muscle.

Key words: Exercise; papillary muscles; aging; rats


 

 

Introduction

The papillary muscles are an integral part of the ventricles and play an important role in their geometry and systolic function1-4 Previous studies of age-related variations in the papillary muscle1-3,5 showed that the density of myocytes, connective tissue and capillaries as well as the cross-sectional area of myocytes and the physical and biochemical characteristics of the papillary muscle are dependent upon age6-13. The most evident age-related effects on the papillary muscle are the loss of muscle cells and an increase in connective tissue9,10.

On the other hand, it has been demonstrated that aerobic exercise alters some of the age-associated changes in the wall of the left ventricular myocardium of rats14-17. However, no data are available on the effect of regular exercise on papillary muscle changes caused by aging. Therefore, in this study, we investigated the effects of aging on the morphology and the density of myocytes, capillaries and connective tissue and the cross-sectional area of myocytes of the papillary muscle of the left ventricle and hypothesized that a long-term moderate exercise program would have a protective role against the deleterious effects of aging.

 

Material and methods

Animals and procedures

Fifteen 6-month-old male Wistar rats, weighing about 420 g each one, were housed in groups of five in a temperature- and light-controlled room and were fed ad lib. The animals were randomly divided into young control (YC), exercise-trained (E) and untrained (U) groups. The E group ran on a treadmill 5 days/wk. The program was progressively increased in intensity and duration of exercise until the 4th week, when the rats ran 1 hour daily at 1.8 km.h-1. This level was maintained throughout the rest of the experimental period. The total training period lasted 28 weeks. The type of exercise used in this experiment can be considered a moderate exercise. The rats from the U group were placed daily for 10 min on the stationary treadmill to provide an equivalent amount of handling. The animals´ handling was approved by our University Ethics Committee, in adherence to the "Guide for the Care and Use of Laboratory Animals" (Institute of Laboratory Animal Resources, National Academy of Sciences, Washington, D. C. 1996) and the Ethical Principles in Animal Experiments of the Brazilian College of Animals Experiments (COBEA).

Tissue sample preparation

At the end of the experiment, the animals from the U and E groups, at 13 months of age (considered to be "middle-aged"4,18), were anesthetized with Pentobarbital sodium (3 mg/100 g body weight, intraperitoneally) and then killed. The additional group of five animals (young control group, YC), was killed at 6 months of age. The hearts were arrested in diastole. The myocardium was perfused through the aorta at a constant pressure of 80 mmHg, using 0.1 M cacodylate buffer (3 min), followed by a 2.5% glutaraldehyde solution diluted in cacodylate buffer.

The animals were heparinized prior to fixation to optimize perfusion-fixation. The heart was then removed, the left ventricle was opened and the papillary muscle was dissected. Each papillary muscle was minced into approximately 3-mm wide and 5-mm long slices. These tissue slices were post-fixed in osmium tetroxide in sodium cacodylate buffer for 1 h. The tissue was dehydrated in graded alcohols, embedded in Epon resin and sectioned, so that the muscle cells of one half of the blocks were cut in cross-section and the other half in longitudinal section. Thin sections for transmission electron microscopy analysis were stained with uranyl acetate19 and lead citrate20. The longitudinal sections were used to evaluate the fixation state of the myocardium by measuring the sarcomere length in the 3 groups.

Stereology

Two randomly chosen blocks from each papillary muscle, in which the myocytes were cut in cross-section were used for the quantitative analysis. The ultrathin sections were placed on a copper grid and 10 randomly chosen fields per block were selected for micrographs, which were taken with a Jeol transmission electron microscope. Low-power (x600) electron micrographs were used for a quantitative analysis of the tissue composition of the left ventricle papillary muscle. Each of the electron micrographs was analyzed using a digital image processing software for the biomedical area (Axio Vision, Zeiss). A total of 300 random electron micrographs were analyzed. A test-system with 72 sampling points was set upon the monitor screen and calibrated.

The myocardium was analyzed concerning the myocytes (my) and the cardiac interstitium (connective tissue [ct]). Volume density was estimated for myocyte (Vv[my]), and connective tissue (Vv[ct]): (VV[structure]: = PP[structure]/PT) (PP is the number of points that hit the structure; PT is the total test points). Length density was estimated for myocardial capillaries, LV[ca]: = 2QA mm/mm3, and QA was also estimated for myocytes (QA [my]). QA is the numerical density per unit area. The numerical density of cell profiles or capillaries in a given area was estimated using the following formula: QA = Σ profiles/given area.

Myocyte mean cross-sectional area (Area[my]) was also determined for each animal and in each group. Results are presented as means ± SEM, computed from the average measurements obtained from each animal.

Statistical analysis

Statistical significance was evaluated by ANOVA and Bonferroni's test, and P values lower than 0.05 were considered to be statistically significant.

 

Results

Effects of exercise

Figure 1 shows the ultramicroscopic appearance of the cross-sectional sections of papillary muscle of the left ventricle in the present groups of rats. Electron microscopic analysis of ultrathin sections revealed the presence of sparse focal areas of interstitial and replacement fibrosis across the myocardium of the papillary muscle of U group of rats at 13 months (Fig. 1C, D) when compared to YC rats (Fig 1A, B). In E rats, the program of continuous treadmill running used in this study reduced the interstitial fibrosis in the old trained papillary muscle (Fig. 1 E, F). The sarcomere length of all the tissue sections was of similar length (approximately 2.1 µm) (Fig. 2) and therefore, they could be compared. Otherwise, they could not have been compared, as different sarcomere lengths would affect the cell diameters and consequently, cell volumes, and thus, volume density.

 



 

General features of the three groups of rats and their left ventricles are shown in Table 1. Body weights of the E and U groups at the start of the training (at 6 months old) were 421±8 g and 427 ± 9 g, respectively (NS). At the end of the running program, body weight of the aged animals (at 13 months old) in the E group (461±10g) was significantly lower than in the aged (also 13 months old) U group (509±11g) (P<0.05). Left ventricular weight increased by 19.5 % in the E group (P<0.05). The LV weight to body weight ratio was significantly higher (P<0.05) in the aged animals from the E group compared to the animals in the U group.

 

 

The quantitative changes in the papillary muscle myocardium with age and the effects of exercise are shown in Table 2. The volume density of myocytes in the aged myocardium of the animals in the U group was 11% lower than that measured in the YC group (P<0.05). As shown in Table 2, alterations in the opposite direction from those seen for the myocyte compartment occurred in the interstitial volume fraction of the tissue. This quantitative constituent was higher in the aged U myocardium, compared with that of the YC group (P<0.05). The length density of the myocardial capillaries was slightly lower in aged U rats when compared with the YC rats (NS). Counts of myocyte profiles per square millimeter showed that a reduction of 20% in the number of myocytes occurred in the U group with age (P<0.05). Aged rats from the U group showed myocyte hypertrophy; the area [my] increased 50% in the aged U animals compared to those from the YC group (P<0.05). However, these age-related changes were not found in the aged rats submitted to the chronic exercise training (Table 2).

 

 

The physical exercise significantly increased the Vv[my], in the aged rats and it was also efficient in decreasing the myocardial fibrosis: Vv[ct] was more than 25% lower in the exercise-trained animals (Table 2). However, it maintained the increase in the Area[my] caused by aging. There was no difference between the U vs. E groups regarding the Area[my]. Physical exercise also apparently improved myocardial vascularization in the E group: LV[ca] was higher in the aged E rats than in the U rats (P<0.05).

 

Discussion

The description of the changes in the structure and function of the heart and the factors that may contribute to reverse the changes that occur with aging is taking on greater importance, as more than 50% of the mortality and the majority of the hospitalizations of the elderly result from cardiovascular diseases21. Therefore, that is the importance of the isolation and identification of the effects of aging alone, from those related to pathological changes.

It is a general belief that as the heart ages, it undergoes a certain degree of hypertrophy9,17,21. However, based on the current results, left ventricular weight did not consistently increase with age, a pattern also observed by others7,22,23, and the present results.

In the present study, it was observed that aging was accompanied by morphoquantitative alterations in the papillary muscle of the left ventricle, the majority of which were attenuated by physical exercise. The quantitative results demonstrate that a significant loss of myocytes occurred with age in the papillary muscle of the left ventricle in the U rats. In this group, it became apparent that a 20% reduction in the numerical density of myocytes was observed. These results are similar to the findings obtained by others, which have shown a reduction in the total number of myocytes in the ventricles of rats from 4 to 12 months7 and from 4 to 15 months9. According to Anversa et al24, a comparable phenomenon occurs in the human heart, as well12,25,26. It is possible that the myocyte hypertrophy that occurred in the aged heart was capable of compensating for the loss of these muscle cells.

Our findings confirm previous reports showing that the decrease in the number of myocytes with age is associated with an increase in interstitial tissue9,10. Collagen accumulation as a function of age was also observed in the human heart11,12. The mechanisms responsible for the loss of myocytes with areas of interstitial and replacement fibrosis in the myocardium are at present unknown7. Ischemia is a likely possibility7,26. However, in the present study, counts of capillary profiles did not show a decreased numerical density in the aged U rats. Under ischemia, the myocardial tissue responds by remodeling27, by myocyte loss due to both apoptosis and necrosis28-31 and/ by reparative fibrosis32,33. According to others34, myocyte loss resulted mainly from apoptosis - genetically programmed cell death. It is possible that these age-related changes in the papillary muscle were responsible for the impairment of both papillary muscle stiffness and LV compliance in advanced aging35.

Changes in the myocyte cross-sectional area17 have been previously used to characterize aging-induced hypertrophy at the cellular level of organization. However, Anversa et al7 demonstrate that myocyte cellular hyperplasia also participates in the hypertrophic response of the myocardium during senescence7. The continued loss of myocytes in the ventricles can be expected to generate a greater workload on the remaining myocytes, which may function as a chronic mechanical stimulus for cellular growth7,11,12,24,36.

The aerobic exercise during aging improved the myocardial vascularization and normalized the myocardial fibrosis. The higher concentration of capillary profiles can be expected to produce an elevation in endothelial luminal surface accessible for oxygen exchange in the tissue and a smaller maximum diffusion distance for oxygen transport to the myocytes7.

 

Conclusion

In conclusion, this study provided morphoquantitative information demonstrating that daily practice of moderate exercise during aging restrains some aging-related deleterious changes in the papillary muscle, such as loss of myocytes, increase in interstitial tissue, and a decreased numerical density of capillary profiles.

Potential Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Sources of Funding

There were no external funding sources for this study.

Study Association

This article is part of the thesis of master submitted by Laura Beatriz Mesiano Maifrino; Rubens Correa Araújo; Cristiane Castro Faccini, Edson Aparecido Liberti, Eliane Florêncio Gama; Antonio Augusto Coppi Maciel Ribeiro e Romeu Rodrigues de Souza, from Universidade de São Judas Tadeu, Universidade de São Paulo e Instituto Dante Pazzanese de Cardiologia.

 

References

1. Gwathmey JK, Slawsky CL, Perrault G, Briggs M, Morgan JP, Wei JY. Effect of exercise conditioning on excitation-contraction coupling in aged rats. J Appl Physiol. 1990; 69 (4): 1366-71.         [ Links ]

2. Thomas DP, McCormick RJ, Zimmerman SD, Vadlamudi RK, Gosselin LE. Aging- and training-induced alterations in collagen characteristics of rat left ventricle and papillary muscle. J Appl Physiol. 1992; 263 (3 Pt 2): H778-83.         [ Links ]

3. Taffet GE, Michael LA, Tate CA. Exercise training improves lusitropy by isoproterenol in papillary muscles from aged rats. J Appl Physiol. 1996; 81 (4): 1488-94.         [ Links ]

4. Berdajs D, Lajos P, Turina MI. A new classification of the mitral papillary muscle. Med Sci Monit. 2005; 11 (1): BR18-21.         [ Links ]

5. Li XX, Lincoln T, Mendelowitz WG, Grossman W, Wei JY. Age-related differences in effect of exercise training on cardiac muscle function in rats. Am J Physiol. 1986; 251: H12-H18.         [ Links ]

6. Klausner SC, Schwartz AB. The aging heart. Clin Geriatr Med. 1985 1(1): 119-41.         [ Links ]

7. Anversa P, Sonnenblick EH. Ischemic cardiomyopathy: pathophysiologic mechanisms. Prog Cardiovasc Dis. 1990; 33 (1): 49-70.         [ Links ]

8. Cafagna D, Ponte E. Morphological and functional aspects of the cardiovascular system related to aging: does "aging heart" exist? Minerva Med. 1997; 88 (12): 491-500.         [ Links ]

9. Aguila MB, Mandarim-de-Lacerda CA, Apfel MI. Stereology of the myocardium in young and aged rats. Arq Bras Cardiol. 1998; 70 (2): 105-9.         [ Links ]

10. Roffe C. Ageing of the heart. B J Biomed Sci. 1998; 55 (2): 136-48.         [ Links ]

11. Debessa CRG, Maifrino LBM, De Souza RR. Age related changes of the collagen network of the human heart. Mech Ageing Dev. 2001; 122 (10): 1049-58.         [ Links ]

12. De Souza RR. Aging of myocardial collagen. Biogerontol. 2002; 3: 325-35.         [ Links ]

13. Lakatta EG. Age-associated cardiovascular changes in health: impact on cardiovascular disease in older persons. Heart Fail Rev. 2002; 7 (1): 29-49.         [ Links ]

14. Douglas PS, O'Toole M. Aging and physical activity determine cardiac structure and function in the older athlete. J Appl Physiol. 1992; 72 (5): 1969-73.         [ Links ]

15. Gates PE, Tanaka H, Graves J, Seals DR. Left ventricular structure and diastolic function with human ageing: relation to habitual exercise and arterial stiffness. Eur Heart J. 2003; 24 (24): 2213-20.         [ Links ]

16. Maifrino LBM., Liberti EA, Castelucci P, De Souza RR. NADPH- diaphorase positive cardiac neurons in the atria of mice: a morphoquantitative study. BMC Neurosci. 2006; 2: 7-10.         [ Links ]

17. Rosa EF, Silva AC, Ihara SSM, Mora OA, Aboulafia J, Nouailhetas VLA. Habitual exercise program protects murine intestinal, skeletal and cardiac muscles against aging. J Appl Physiol. 2005; 99: 1569-75.         [ Links ]

18. Gerrity RG, Cliff WJ. The aortic tunica intima in young and aging rats. Exp Mol Path. 1972; 16 (3): 382-402.         [ Links ]

19. Watson ML. Staining of tissue sections for electron microscopy with heavy metals. J Bioph and Biochem Cytol. 1958; 4 (4): 475-8.         [ Links ]

20. Reynolds ES. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol. 1963; 17: 208-12.         [ Links ]

21. Lakatta EG, Yin FC. Myocardial aging: functional alterations and related cellular mechanisms. Am J Physiol. 1982; 242 (6): H927-41.         [ Links ]

22. Capasso JM, Malhotra A, Remily RM, Scheuer J, Sonnenblick EH. Effects of age on mechanical and electrical performance of rat myocardium. Am J Physiol. 1983; 245 (1): H72-81.         [ Links ]

23. Capasso JM, Malhotra A, Scheuer J, Sonnenblick EH. Myocardial biochemical, contractile, and electrical performance after imposition of hypertension in young and old rats. Circ Res. 1986; 58 (4): 445-60.         [ Links ]

24. Anversa P, Hiler B, Ricci R, Guideri G, Olivetti G. Myocyte cell loss and myocyte hypertrophy in the aging rat heart. J Am Coll Cardiol. 1996; 8 (6): 1441-8.         [ Links ]

25. Unverferth DV, Baker PB, Arn AR, Magorien RD, Fetters J, Leier CV. Aging of the human myocardium: a histologic study based upon endomyocardial biopsy. Gerontol. 1986; 32 (5): 241-51.         [ Links ]

26. Anversa P, Leri A, Li B, Liu Y, Di Somma S, Kajstura J. Ischemic cardiomyopathy and the cellular renin-angiotensin system. J Heart Lung Transp. 2000; 19 (8 Suppl): S1-11.         [ Links ]

27. Anversa P, Zhang X, Li P, Olivetti G, Cheng W, Reiss K, et al. Ventricular remodeling in global ischemia. Cardioscience. 1995; 6 (2): 89-100.         [ Links ]

28. Anversa P, Cheng W, Liu Y, Leri A, Redaelli G, Kajstura J. Apoptosis and myocardial infarction. Basic Res Cardiol. 1998; 93 (Suppl. 3): 8-12.         [ Links ]

29. Díez J, Fortuño MA, Ravassa S. Apoptosis in hypertensive heart disease. Curr Opin Cardiol. 1998; 13 (5): 317-25.         [ Links ]

30. Mandarim-de-Lacerda CA, Pereira LMM. Numerical density of cardiomyocytes in chronic nitric oxide synthesis inhibition. Pathobiol. 2000; 68: 36-42.         [ Links ]

31. Mani K, Kitsis RN. Myocyte apoptosis: programming ventricular remodeling. J Am Coll Cardiol. 2003; 41(5): 761-4.         [ Links ]

32. Weber KT, Brilla CG, Campbell SE, Reddy HK. Myocardial fibrosis and the concepts of cardioprotection and cardioreparation. J Hypertens Suppl. 1992; 10 (5): S87-94.         [ Links ]

33. Weber KT, Brilla CG, Janicki JS. Myocardial fibrosis: functional significance and regulatory factors. Cardiovasc Res. 1993; 27 (3): 341-8.         [ Links ]

34. Brömme HJ, Holtz J. Apoptosis in the heart: when and why? Mol Cell Biochem. 1996; 163-164: 261-75.         [ Links ]

35. Rozenberg S, Tavernier B, Riou B, Swynghedauw B, Page CL, Boucher F, et al. Severe impairment of ventricular compliance accounts for advanced age-associated hemodynamic dysfunction in rats. Exp Gerontol. 2006; 41 (3): 289-95.         [ Links ]

36. Yin FC, Spurgeon HA, Rakusan K, Weisfeldt ML, Lakatta EG. Use of tibial length to quantify cardiac hypertrophy: application in the aging rat. Am J Physiol. 1982; 243 (6): H941-7.         [ Links ]

 

 

Mailing address:
Laura Beatriz Mesiano Maifrino
Rua Pirandello, 770 casa 08 - Brooklin Paulista
04.623-000, São Paulo, SP - Brazil
E-mail: lmaifrino@uol.com.br

Manuscript received April 02, 2008; revised manuscript received May 13, 2008; accepted May 15, 2008.