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Arquivos Brasileiros de Cardiologia

Print version ISSN 0066-782X

Arq. Bras. Cardiol. vol.97 no.5 São Paulo Nov. 2011

http://dx.doi.org/10.1590/S0066-782X2011001400016 

CASE REPORT

 

Lactococcus garvieae endocarditis: first case report in Latin America

 

 

Tatiana Franco HirakawaI,II; Fernando Augusto Alves da CostaI,II; Marcos Cairo VilelaI,II; Micheli RigonI,II; Henry AbensurII; Maria Rita Elmor de AraújoII

IFGM - Clínica Paulista de Doenças Cardiovasculares, São Paulo, SP - Brasil
IIHospital Beneficência Portuguesa de São Paulo, São Paulo, SP - Brasil

Mailing address

 

 


ABSTRACT

Lactococcus garvieae, an emerging zoonotic pathogen, is responsible for mastitis in rodents and sepsis in fish. Although deemed opportunistic and hardly ever causing infections in humans, its incidence is probably underestimated due to the difficulty in diagnosis. There are very few reports of osteomyelitis, liver abscess, and peritonitis, and only nine cases of endocarditis described in worldwide literature. We describe the first case of Lactococcus garvieae endocarditis in Latin America, in a female patient with metallic prosthetic heart valve who presented with daily fever, chills, Osler nodes and six positive blood cultures for Lactococcus garvieae, which met Duke's criteria for the diagnosis of "definitive infective endocarditis".

Keywords: Endocarditis, bacterial; diagnosis; Lactococus garvieae; heart valve prosthesis.


 

 

Introduction

Lactococcus garvieae, an emerging zoonotic pathogen, was originally isolated in mastitis in rodents and is responsible for sepsis in fish; it is deemed opportunistic and shows low virulence in humans, with very few reports of endocarditis (nine cases in the worldwide literature)1-7, osteomyelitis8, liver abscess9, sepsis, and peritonitis5. However, the difficult differential diagnosis with Enterococcus may lead to underestimation of its incidence and real importance. In the present study, we report the first case of Lactococcus garvieae endocarditis in Latin America, the tenth in the worldwide literature.

 

Case report

A 58-year-old female Caucasian patient, a former smoker previously hypertensive, with type-2 diabetes, dyslipidemia, underwent mitral valve replacement by a metallic prosthetic valve six months earlier due to severe mitral stenosis of rheumatic etiology. Upon arrival at the hospital, the patient had a history of daily fever for six days (38.5º C), chills, diaphoresis, erythematous nodules in hands and legs, myalgia and weakness. As regards her nutritional habits, she often consumed several types of fish and reported having eaten white cheese recently. Despite denying dental treatment in the past few months, the patient had a dental prosthesis and had had a gingival perforation with a "fish bone" five days prior to the onset of symptoms.

On admission, she had fever (38.2º C), Osler nodes on her left hand and legs, and poor dentition. The remainder of her physical examination was unremarkable. Laboratory tests showed alterations in inflammatory tests, with PCR = 81.9 and VHS = 47.1, with no leukocytosis. No focus of infection was identified, despite extensive diagnostic investigation.

In samples for blood cultures drawn from three different sites, Gram-positive cocci were isolated and further identified as Lactococcus garvieae by means of biochemical tests and confirmed by genetic studies. The pathogen was isolated again in three other blood cultures samples drawn five days later. The antibiotic susceptibility test showed resistance only to clindamycin, and sensitivity to penicillin, gentamicin, vancomycin and other antibiotics.

Two transesophageal echocardiograms were performed, revealing absence of vegetation and competent mitral valve prosthesis. However, according to Duke's modified criteria, the patient was diagnosed with endocarditis and was treated with vancomycin 1 g every 12 hours for 28 days, due to confirmed penicillin allergy. There was complete remission of signs and symptoms, as well as normalization of laboratory tests; control blood cultures were negative.

In an attempt to identify the source of the infection, the white cheese consumed by the patient was sent for microbiologic testing. In the search for an "entrance door", due to the family history of intestinal polyposis, the patient also underwent barium enema. However, both tests were normal.

The patient was discharged with no symptoms after 30 days of hospitalization, and was referred for cardiology outpatient follow-up.

 

Discussion

Lactococcus garvieae is one of the eight species belonging to the Lactococcus genus, originally known as the lactic group of the Streptococcus genus, from which it was split in 1985, after genetic analysis10.

Formed by facultatively anaerobic catalase-negative Gram-positive cocci, these bacteria produce lactic acid, which provide them with fermentation ability and bactericidal property; thus, it is used in the food industry for fermentation and preservation of food10.

However, it is known that some species may be pathogenic to animals and humans, especially Lactococcus garvieae and Lactococcus Lactis10.

Considered an emerging zoonotic pathogen, Lactococcus garvieae is responsible for mastitis in rodents, and sepsis in fish. Infected fish which do not develop the disease may contribute to dissemination5.

It is considered an uncommon pathogen in humans; it is opportunistic and shows low virulence. Very few cases have been described in the literature with Lactococcus garvieae being the causal agent of endocarditis1-7, osteomyelitis8, liver abscess9, sepsis and peritonitis5. There are nine cases of endocarditis reported in worldwide literature: in four of them prosthetic valves1,3 are affected, and in the other five, native heart valves are2,4,5,7.

This is the first case report of Lactococcus garvieae endocarditis in Latin America in a patient with metallic prosthetic valve in the mitral position who met the modified Duke criteria for the diagnosis of "definitive endocarditis" (Table 1).

 

 

Pathogenicity and infectivity of Lactococcus garvieae remain inconclusive. It is known that lactococci are not typically a part of the human flora3, but the intake of milk or contaminated fish may be sources of infection5. They have also been isolated in manufactured food due to their use in food products. However, gastric acidity, pancreatic enzymes, gallbladder and intestinal secretions, peristalsis, and epithelial cell integrity are protective factors against oral and gastrointestinal infections. The existence of gastrointestinal disorders such as ulcers, polyps or diverticula may act as facilitators of bacterial infectivity.

In the case described, the source of infection may have been the fish routinely consumed by the patient; the wound may have been caused by the "fish bone" and her poor dentition which may both been the entrance door. However, no data confirm this hypothesis.

It is difficult to distinguish L. garvieae from Enterococcus, because their phenotypes are similar. It is also challenging to distinguish Lactococcus garvieae from L. lactis. Some studies suggest the use of antibiotic susceptibility tests, because L. garvieae is resistant to clindamycin, whereas L. lactis is always sensitive to this antibiotic: this proved true in the present case.

In relation to the echocardiogram, few reports are found in the literature of patients presenting with vegetations in the native valve2, and cases without abnormalities both in the transthoracic and transesophageal test of patients with endocarditis in prosthetic valves8, as in the present case report.

The gold-standard test for the identification of Lactococcus gavieae, despite its high cost, is the genetic study with confirmation of its DNA sequence10.

 

Conclusion

Lactococcus garvieae endocarditis is extremely uncommon, this being the tenth case described in worldwide literature, and its pathogenesis remains inconclusive. Possible sources of infection are contaminated milk and fish, with reports of growth of these bacteria in manufactured food. Gastrointestinal comorbidities and poor oral conditions may facilitate infectivity of this pathogen. Prosthetic heart valves are also predisposing factors, and this implies the need to advise patients on the importance of oral hygiene and dental treatment in the prevention of endocarditis. Gastrointestinal disorders should also be investigated whenever necessary.

Phenotypic similarities between Lactococcus and Enterococcus make it difficult to establish a microbiological diagnosis, so that genetic studies are still necessary for the definite identification of Lactococcus garvieae. The fact that these tests are unavailable in most of the medical centers worldwide probably cause Lactococcus garvieae to be underdiagnosed, so its clinical significance may be far greater than is thought.

Potential Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Sources of Funding

There were no external funding sources for this study.

Study Association

This study is not associated with any post-graduation program.

 

References

1. Furutan NP, Breiman RF, Fischer MA, Facklam RR. Lactococcus garvieae infections in humans: a cause of prosthetic valve endocarditis. In: 91st General Meeting American Society for Microbiology. Washington, DC; 1991. p. 109, C-297 [Abstract]         [ Links ].

2. Fefer JJ, Ratzan KR, Sharp SE, Saiz E. Lactococcus garvieae endocarditis: report of a case and review of the literature. Diagn Microbiol Infect Dis. 1998;32(2):127-30.         [ Links ]

3. Fihman V, Raskine L, Barrou Z, Kiffel C, Riahi J, Berçot B, et al. Lactococcus garvieae endocarditis: identification by 16S rRNA and sodA sequence analysis. J Infect. 2005;52(1):e3-6.         [ Links ]

4. Vinh DC, Nichol KA, Rand F, Embil JM. Native-valve bacterial endocarditis caused by Lactococcus garvieae. Diagn Microbiol Infect Dis. 2006;56(1):91-4.         [ Links ]

5. Wang CY, Shie HS, Chen SC, Huang JP, Hsieh IC, Wen MS, et al. Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. Int J Clin Pract. 2007;61(1):68-73.         [ Links ]

6. Yiu KH, Siu CW, To KK, Jim MH, Lee KL, Lau CP, et al. A rare cause of infective endocarditis; Lactococcus garvieae. Int J Cardiol. 2007;114(2):286-7.         [ Links ]

7. Li WK, Chen YS, Wann SR, Liu YC, Tsai HC. Lactococcus garvieae endocarditis with initial presentation of acute cerebral infarction in a healthy immunocompetent man. Intern Med. 2008;47(12):1143-6.         [ Links ]

8. James PR, Hardman SM, Patterson DL. Osteomyelitis and possible endocarditis secondary to Lactococcus garvieae: a first case report. Postgrad Med J. 2000;76(895):301-3.         [ Links ]

9. Mofredj A, Baraka D, Kloeti G, Dumont JL. Lactococcus garvieae septicemia with liver abscess in an immunosuppressed patient. Am J Med. 2000;109:513-4.         [ Links ]

10. Facklam R, Elliott JA. Identification, classification, and clinical relevance of catalase-negative, Gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev. 1995;8(4):479-95.         [ Links ]

 

 

Mailing Address:
Fernando Augusto Alves da Costa
Praça Amadeu Amaral, 47 cj 12A - Bela Vista
01327-010 - São Paulo, SP - Brasil
E-mail: alvesdacosta@uol.com.br, faacosta@cardiol.br

Manuscript received June 05, 2010; revised manuscript received August 20, 2010; accepted September 09, 2010.

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