Abstract
Exogenous concentrations of bean seed extract prepared from seeds pretreated in aerated water, homogenized in Veliky and Martin's 67-V salt solution, filtered, and added to the culture medium at proper concentrations promote callus proliferation, root morphogenesis, and shoot morphogenesis in leaf explains of Phaseolus vulgaris var. Bico de Ouro. The activity of the bean seed factor is dependent upon the period of pretreatment in aerated water.
The induction of root and shoot morphogenesis in Phaseolus vulgaris tissue cultures* * Supported by grants from Comissão Nacional de Energia Nuclear (CNEN) and Fundação de Amparo à Pesquisa do Listado de São Paulo (FAPESP) -Brazil.
O.J. CrocomoI; J.E:. PetersII; W.R. SharpII
IDepartment of Chemistry and CENA - E.S.A. "Luiz de Queiroz" - USP, Piracicaba, SP - Brazil
IIDepartment of Microbiology, The Ohio State University. Columbus, Ohio 43210 U.S.A
ABSTRACT
Exogenous concentrations of bean seed extract prepared from seeds pretreated in aerated water, homogenized in Veliky and Martin's 67-V salt solution, filtered, and added to the culture medium at proper concentrations promote callus proliferation, root morphogenesis, and shoot morphogenesis in leaf explains of Phaseolus vulgaris var. Bico de Ouro. The activity of the bean seed factor is dependent upon the period of pretreatment in aerated water.
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LITERATURE CITED
Received for publication in 10/10/1975.
- CROCOMO, O.J., PETERS, J.E. & SHARP, W.R., 1976. Interactions of Phytohormones on the control of growth and root morphogenesis in cultured Phaseolus vulgaris leaf explants. Turrialba, 26:232-236.
- DEBERGH, P. & NITSCH., C, 1973. Premiers résultats sur la culture in vitro de grains de pollen isolés chez la Tomate. C.R. Acad. Sc. Paris t. 276 Serie D. 1281-1284.
- GAMBORG, O.L., 1966. Aromatic metabolism in plants. II. Enzymes of shikimate pathway in suspension cultures of plant cells. Can. J. Biochem., 44:791-799.
- HILDEBRANDT, A.C., WILMAR, J.C., JOHNS, H. & RIKER, A.J., 1963. Growth of edible chlorophyllous plant tissues in vitro. Amer. J. Bot., 50: 248-254.
- JEFFS, R.A. & NORTHCOTE, D.H., 1967. The influence of IAA and sugar on the pattern of induced differentiation in plant tissue culture. J. Cell. Sci., 2:77-88.
- LAMPORT, D.T.A., 1964. Cell suspension cultures of higher plants: isolation and growth energetics. Exp. Cell Res., 33:195-206.
- LIAU, D.F. & BOLL, W.G., 1971. Growth and patterns of growth and division of cell suspension cultures of bush beans (P. vulgaris cv. Contender). Can. J. Bot., 49:1131-1139.
- MEHTA, A.R., HENSHAW, G.G. & STREET, H.E., 1967. Aspects of growth in suspension cultures of Phaseolus vulgaris L. and Linum usitatissium L. Indian J. Plant Physiol., 10:44-53.
- PETERS, J.E., CROCOMO, O.J. & SHARP, W.R., 1976. Effect os caffeine and nicotine on the callus growth and root morphogenesis of Phaseolus vulgaris tissue cultures. Turrialba, 26: 337-341.
- VELIKY, I.A., 1972. Synthesis of carboline alkaloids by plant cell cultures. Phytochemistry, 11:1405-1406.
- VELIKY, I.A. & MARTIN, S.M., 1970. A fermenter for plant cell suspension cultures. Can. J. Microbiol., 16:223-226.
Publication Dates
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Publication in this collection
30 May 2012 -
Date of issue
1975
History
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Received
10 Oct 1975
