Determinação da digestibilidade de nutrientes através da técnica do "saco de nylon" mais pepsina

Determination of nutrients digestibility using nylon bag technique plus pepsin solution

ARTIGOS

Determinação da digestibilidade de nutrientes através da técnica do "saco de nylon" mais pepsina

Determination of nutrients digestibility using nylon bag technique plus pepsin solution

M.L.V. Bose^I; E.M.R.de Carvalho^II

^IDepartamento de Zootecnia da E.S.A."Luiz de Queiroz", da Universidade de São Paulo - 13.400 - Piracicaba, SP

^IICurso de Pós-Graduação em Nutrição Animal e Pastagens da E.S.A."Luiz de Queiroz"

RESUMO

Procurou-se verificar se a digestão de alimento em saco de "nylon", acrescida por tratamento com pepsina, seria equivalente à digestão obtida pelo método direto clássico, o de coleta total de fezes, já determinado anteriormente com ovinos. Seguiu-se um delineamento experimental em blocos ao acaso, com 6 repetições e 4 tratamentos, ou seja, tratamento A: ração contendo 100% de feno de capim de Rhodes; B: 85% de feno de Rhodes mais 15% de farelo de côco; C: 70% de feno de Rhodes mais 30% de farelo de côco; e D: 100% de farelo de côco. O animal fistulado utilizado para teste foi um bovino adulto, macho, de raça holandesa; o tempo de permanência do saco de "nylon" no rume foi de 72 horas; e a dieta consistiu em feno de Rhodes à vontade mais 2kg de farelo de coco por dia. Os resultados foram analisados através de análise da variância e curvas de regressão, e a comparação entre métodos de digestibilidade pelo teste de Tukey. Houve influência da associação de alimentos sobre a digestibilidade da fibra bruta (FB) e dos extrativos não nitrogenados (ENN), mas não sobre as demais frações - matéria seca (MS), extrato etéreo (EE) e proteína bruta (PB). A digestibilidade da fibra diminuiu com a inclusão e o acúmulo do nível de farelo na dieta. Não houve equivalência consistente entre os métodos testados de digestiblidade, variando ou não conforme o nutriente e o tratamento considerado. De modo geral, a inclusão de aproximadamente 15% de farelo de côco (tratamento B) favoreceu o melhor aproveitamento da dieta.

Termos para indexação: digestibilidade, técnica do saco de nylon mais pepsina, farelo de côco

ABSTRACT

"Nylon bag" technique, followed by 48 hours of chloridric pepsin digestion, was tried for determination of the digestibility of proximate nutrients of Rhodes grass hay (Chloris gayana, Kunth, cv. callide) and coconut caque meal, and compared with results from "in vivo" method. In a randomized blocks design, 100% of Rhodes grass, and mixtures of 85% and 70% of the grass hay with 15%, and 30% of coconut cake, and 100% coconut cake, made up four treatments (A, B, C and D, respectively). The coefficients of digestibility for Dry Matter and for proximate nutrients in treatments A, B and C, determined through nylon bags in bovine rumen, were compared to the coefficients determined by total faces collection, in sheep. It was observed and concluded that: a) Position of samples along the supoort-bars inside rumen, as well as dfferent periods of animal usage, had no influence on the results; b) The calculated proportions of coconut in the mixture to obtain best digestibility were 17.48% for Dry Matter, 21.10% for Ether Extract, 29.01% for Crude Protein, and 16.49% for Non Nitrogenous Extrat. Crude Fiber digestibility decreased linearly as the coconut proportion in the mixture increased; c) "Nylon bag" and "in vivo" methods gave similar coefficients of digestibility for Crude Fiber for all treatments; for other nutrients, however, there were differences depending on treatments; d) TDN values for coconut cake meal, determined through "nylon bags" were 68.76% taking the average coefficients of digestibility from treatments B and C, and 68.08% from treatment D; "in vivo", the average from B and gave 71.06% of TDN; such a small difference in NDT suggests to have had no interference of foods on digestibility; e) NDT calculate for Rhodes grass hay was 58.38% "in nylon bags", and 44.81% "in vivo", when treatment A was the only one considered; thus, it could be concluded that nylon bag plus pepsin method did not work for Rhodes hay.

Index terms: digestibility, nylon bag plus, pepsin technique, coconut caque meal.

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Entregue para publicação em: 17/02/88

Aprovado para publicação em: 21/06/88.

• AGAR, A.; GÁLVEZ, J.V.; ZAERA, E.; GONZALEZ, V. Comparación entre dos tecnicas para la determinación de la digestibilidad de los forrages. In: Annales de le Institute Nacional de Investigaciones Agrícolas, Madrid, 3:27-29. 1972.
• ARCHIBALD, J.G.; FENNER, H.; OWEN Jr.,D.F.; BARNES, H. D. Measurement of the nutritive value of alfafa and timothy hay by varied techniques. Journal of Dairy Science, Champaign, 44:2232-41, 1961.
• CASTILLO, L.S.; GLORIA, A.; GERPACIO, A.L.; GATAPIA, A. R.; AGLIBUT, F.B.; MACAM, B.T. Effects of high levels of copra meal on the quantity and quality of milk of cows and carabaos. Philippine Agriculturist, Los Baños, 45:385-93, 1961.
• CHENOST, M.; GRENET, E. ; DeMARQUILLY, C.; JARRIGE, R. The use of the nylon bag technique for the study of forage digestion in the rumen for predicting feed value. In: INTERNATIONAL GRASSLAND CONGRESS, 11, Austrália, 1971. p.697-701.
• CHURCH, D.C. Digestive physiology and nutrition of ruminants. 2.ed. Oregon, O&B Books, 1975. 350p.
• CIPOLLINI, M.A. et alii. Significance of the differences in digestibility of feeds by cattle and sheep. Journal of Animal Science, Champaign, 10: 337-43, 1951.
• DeMARQUILLY, C. & CHENOST, M. Etude de la digestion des fourrages dans le rumen par la methode des sachets de nylon, liaisons avec la valeur  alimentaire. Annates de Zootechnie, Versailles, 18:419-36, 1969.
• ERWIN, E.S. & ELLISTON, N.G. Rapid method of determining digestibility of concentrates and roughages  in cattle. Journal of Animal Science, Champaign, 18: 1518-1959 (Abstract).
• FIGROID, W.; HALE, W.H.; THEURER, B. An evaluation of the nylon technique for estimating rumen utilization of grains. Journal Animal Science, Champaign, 35: 113-20, 1972.
• GOMIDE, J.A. A técnica de fermentação ruminal  "in vitro" na avaliação de forragens. Revista da Sociedade Brasileira de Zootecnia, Viçosa, 3:210-24, 1974.
• GONZALEZ, L.R. Bromatologia zootecnica y alimentación animal. Barceola, Salvat, 1953.
• HOPSON, J.D.; JOHNSON, R.J.; DEHORTTY, B.A. Evaluation of the dracon bag technique as a method for measuring cellulose digestibility and rate of forage digestion. Journal of Animal Science. Champaign, 22:448-53, 1963.
• LOWREY, R.S. The nylon bag technique for the estimation of forage quality. In: NATIONAL CONFERENCE OF FORAGE QUALITY EVALUATION ON UTILIZATION. Lincoln. 1970. Proceedings, p.1-12.
• LUSK, J.W.; BROWING, C.B.; MILES, J.T. Small-sample in vivo cellulose digestion procedure for forage evaluation. Journal of Dairy Science, Champaign,45: 69-73, 1962.
• McGILLIARD, A.D.; DUNCAN, C.W.; HUFFMAN, C.F. Further studies of the bovine digestive tract using a duodenal fistula technique. Journal of Animal Science, 16:1107 (Abstract).
• MARWAHA, S.R.; KOCHAR, A.S.; SHATIA, I.S. An "in vivo" study on the effect of different types of dietary lipids on the microbial population in the rumen of zebu cattle. Indian Journal of Nutrition and Dietetics, Coimbatore, 10:27-30, 1973.
• MONSON, W.G.; LOWREY, R.S.; FORBES JUNIOR, I. In vivo nylon bag vs. two stage in vitro digestion: comparision of two techniques for estimation dry - matter digestibility of forages. Agronomy Journal, Madison, 61:587-9, 1969.
• NEATHERY, M.W. Dry matter dispearance of roughages in nylon bags suspended in the rumen. Journal of Dairy Science, Champaign, 52:74-8, 1969.
• NEATHERY, M.W. Conventional digestion traias vs. nylon bag technique for determining seasonal difference in quality of Midland Bermudagrass forage. Journal of Animal Science, Champaign, 34:1075-84, 1972.
• PEIXOTO, A.M. Fundamentos de nutrição animal. Piracicaba, ESALQ, 1972.
• RIEWE, M.E. & LIPPKE, H. Considerations in determining the digestibility of harvested forages. In: NATIONAL CONFERENCE ON FORAGE QUALITY EVALUATION  ON UTILIZATION. Lincoln, 1970. Proceedings, p.1-17.
• SOBRAL, J.P. Composição química e digestibilidade do farelo de côco. Piracicaba, 1976. 63p. (Mestrado-Escola Superior de Agricultura "Luiz de Queiroz"/USP).
• TILLEY, J.M.A. & TERRY, R.A. A two-stage technique for the "in vitro" digestion of forage crops. Journal of the British Grassland Society, Aberystwyth, 18: 104-11, 1963.
• THOMAS, O.A. & SCOTT, M.L. Coconut oil meal as a protein supplement in practical poultry diets. Poultry Science, Champaign, 41:477-85, 1962.
• VAN KEUREN, R.W. & HEINEMAN, W.W. Study of a nylon bag technique for in vivo estimation of forage digestibility. Journal of Animal Science, Champaign, 21:340-5, 1962.
• WALDO, D.R. Factors influencing the voluntary intake of forages. In: NATIONAL CONFERENCE ON FORAGE QUALITY EVALUATION AND UTILIZATION, Lincoln, 1970. Pro ceedings, p.1-22.
• WOODROOF, J.G. Coconuts: production, processing, products. Westport, Conn. Avi. Publ., 1970. cap.4, p.51.

Datas de Publicação

• Publicação nesta coleção
  19 Ago 2010
• Data do Fascículo
  1988

Histórico

• Recebido
  17 Fev 1988
• Aceito
  21 Jun 1988