Atividades de transaminase glutamico-oxaloacético e transcarbamilase de ornitina em folhas de feijoeiro (Phaseolus vulgaris L.) afetadas pela deficiência de potássio

Gutierrez, L.E.; Crocomo, O.J.

doi:10.1590/S0071-12761988000100040

Resumos

Folhas de feijoeiro (Phaseolus vulgaris L.) normais e deficientes em potássio dos cultivares Rosinha Precoce e Goiano Precoce foram analisadas para determinação das atividades de transaminase glutâmico-oxaloacético (E.C. 2.6.1.1) e transcarbamilase de ornitina (E.C. 2.1.3.3). Em plantas deficientes em potássio houve aumento na atividade das duas enximas estudadas e nos dois cultivares. Esses resultados explicariam o acúmulo de citrulina e arginina em plantas deficientes em potássio devido a maior atividade da transcarbamilase de ornitina, que possivelmente seria um mecanismo de reduzir a toxidez por amônia.

transaminase glutâmico-oxaloacético; transcarbamilase de ornitina; feijoeiro; Phaseolus vulgaris

Leaves of bean plants (Phaseolus vulgaris L.) deficient in potassium and the proper control of two cultivars were analysed in relation to ornithine transcarbamylase (E.C. 2.1.3.3) and glutamic-oxaloacetic transaminase (E.C. 2.6.1.1) activities. Leaves of bean plants grown under potassium deficiency showed higher activities of both enzymes. These dada suggest that the accumulation of citrulline and arginine in potassium deficient plants was due to the higher activity of ornithine transcarbamylase, probably as a mechanism to reduce ammonium toxicity.

ornithine transcarbamylase; glutamic-oxaloacetic transaminase; bean; Phaseolus vulgaris

ARTIGOS

Atividades de transaminase glutamico-oxaloacético e transcarbamilase de ornitina em folhas de feijoeiro (Phaseolus vulgaris L.) afetadas pela deficiência de potássio

Ornithine transcarbamylase and glutamic-oxaloacetic transaminase activities of bean leaves (Phaseolus vulgaris L.) as affected by potassium deficiency

L.E. Gutierrez; O.J. Crocomo

Departamento de Química da E.S.A. "Luiz de Queiroz" da Universidade de São Paulo - 13.400 - Piracicaba, SP e CEBTEC/FEALQ

RESUMO

Folhas de feijoeiro (Phaseolus vulgaris L.) normais e deficientes em potássio dos cultivares Rosinha Precoce e Goiano Precoce foram analisadas para determinação das atividades de transaminase glutâmico-oxaloacético (E.C. 2.6.1.1) e transcarbamilase de ornitina (E.C. 2.1.3.3). Em plantas deficientes em potássio houve aumento na atividade das duas enximas estudadas e nos dois cultivares. Esses resultados explicariam o acúmulo de citrulina e arginina em plantas deficientes em potássio devido a maior atividade da transcarbamilase de ornitina, que possivelmente seria um mecanismo de reduzir a toxidez por amônia.

Termos para indexação: transaminase glutâmico-oxaloacético, transcarbamilase de ornitina, feijoeiro, Phaseolus vulgaris.

ABSTRACT

Leaves of bean plants (Phaseolus vulgaris L.) deficient in potassium and the proper control of two cultivars were analysed in relation to ornithine transcarbamylase (E.C. 2.1.3.3) and glutamic-oxaloacetic transaminase (E.C. 2.6.1.1) activities. Leaves of bean plants grown under potassium deficiency showed higher activities of both enzymes. These dada suggest that the accumulation of citrulline and arginine in potassium deficient plants was due to the higher activity of ornithine transcarbamylase, probably as a mechanism to reduce ammonium toxicity.

Index terms: ornithine transcarbamylase, glutamic-oxaloacetic transaminase, bean, Phaseolus vulgaris.

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Recebido para publicação em: 10.11.88

Aprovado para publicação em: 22.03.89

ACHITUV, M. & BAR-AKIVA, A. Glutamic-oxaloacetic transamine in leaves of phosphorus deficient citrus leaves. Physiologia Plantarum, Copenhagen, 36:368-73 , 1976.
ACHITUV, M. & BAR-AKIVA, A. Nitrogen accumulation induced by phosphorus deficiency in citrus plants. Scientia Horticulturae, Amsterdam, 1:251-62, 1973.
BASSO, L.C. Significado bioquímico da deficięncia de potássio na formaçăo de aminas em gergelim (Sesamum indicum) Botucatu, 1973. 85p. (Doutorado - Faculdade de Cięncias Médicas e Biológicas de Botucatu).
BASSO, L.C. & SMITH, T.A. Effect of mineral deficiency on amine formation in higher plants. Phytochemistry, Oxford, 13:875-83, 1974.
BESFORD, R.T. & HOBSON, G.E. Effect of potassium nutrition on some enxymes from ripening Lycopersicon esculetun fruit. Phytochemistry, Oxford, 14:57-99,1975.
BROWN, G.W. & COHEN, P.P. Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver. Journal of Biological Chemistry, Baltimore, 234:1769-74, 1959.
CATTINI, M.A. Contribuiçăo ao estudo do papel fisiológico e bioquímico do potássio em plantas de feijăo (Phaseolus vulgaris L. cv. Goiano precoce). Piracicaba, 1974. 67p. (Mestrado - Escola Superior de Agricultura "Luiz de Queiroz"/USP).
COBRA NETTO, A.
CROCOMO, O.J. & BASSO, L.C. Accumulation of putrescine and related amino acids in potassium deficient Sesamum. Phytochemistry, Oxford, 13:2659-65, 1974.
CROCOMO, O.J.; CATTINI, M.A.; ZAGO, E.A. Acumulo de aminas e aminoácidos em relaçăo ao nível de potássio em folhas de feijăo (Phaseolus vulgaris L.). Arquivos de Biologia e Tecnologia, Curitiba, 17:93-102, 1974.
EATON, S.A. Effects of potassium deficiency on growth and metabolism of sunflower plants. Botanical Gazette, Chicago, 114:165-80, 1952.
ELLIS, R.J. & DAVIES, D.D. Clutamic-oxaloacetic transaminase of cauliflower. I, Purification ans specificity. Biochemical Journal, London, 78:615-23, 1961.
GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilaçăo do nitrogęnio afetada pela deficięncia de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). Săo Paulo, 1977. 108p.(Doutorado - Instituto de Química/USP).
GUTIERREZ, L.E.; CROCOMO, O.J.; BASSO, L.C. Efeito da deficięncia de potássio sobre alguns aspectos metabólicos em feijoeiro (Phaseolus vulgaris L.). Revista de Agricultura, Piracicaba, 53(4):209-16, 1978.
HARPER, J.E. & PAULSEN, G.M. Nitrogen assimilation and protein synthesis in wheat seedlings as affected by mineral nutrition. I. Macronutrients. Plant Physiology, Lancaster, 44:69-74, 1969.
KASTING, R. & DELWICHE,C.C. Orinithine, citrulline and arginine metabolism in water melon seedlings. Plant Physiology, Lancaster, 33:350-4, 1958.
KLECZOWSKI, K. & COHEN, P.P. Purification of ornithine transcarbamylase from pea seed lings. Archives of Biochemistry and Biophysics, New York, 107:211-8 , 1964.
LOWRY, O.H.; ROSEBROYGH, N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193:265 -75, 1951.
MALLERY, C.H. Isoenzyme activities during the early stages of Allium radicle germination. Physiologia Plantarum, Copenhagen, 26:136-42, 1972.
MATSUM0T0, H. WAKIUCHI, N. Changes in ATP in cucumber leaves during ammonium toxicity. Zeitschrift fur Pflanzenphysiologie, Jena, 73:82-5, 1974. Apud Chemical Abstracts, Easton, 81:115-468y.
PATNAIK, R.; BARKER, A.V.; MAYNARD, D.N. Effects of ammonium and potassium ions on some physiological and biochemical process of excised cucumber cotyledons. Physiologia Plantarum, Copenhagen, 27:32-6, 1972.
PIMENTEL GOMES, F. Curso de estatística experimental. 4.ed. Săo Paulo, 1970. 430p.
SMITH, T.A. The occurrence, metabolism and functions of amines in plants. Biological Review of the Cambridge Philosophical Society, Cambridge, 46:201-41, 1971.
SPLITTSTOESSER, W.E. Metabolism of arginine by aging in 7 days old punpkin seedlings. Plant Physiology , Lancaster, 44:361, 1969.
SPLITTSTOESSER, W.E. & STEWART, S.A. Distribution and isoenzymes of aspartate aminotransferase in cotyledons of germinating punpkins. Physiologia Plantarum, Copenhagen, 23:1119-26, 1970.
WAKIUCHI, N.; MATSUMOTO, H.; TAKAHASHI, E. Changes of some enzymes activities of Cucumber during ammonium toxicity. Physiologia Plantarum, Copenhagen, 24:248-51, 1971.
WONG, K.F. & COSSINS, E.A. Studies of the particulate and soluble aspartate amino transferase in germinating pea cotyledons. Phytochemistry, Oxford , 8:1327-38, 1969.

Datas de Publicação

Publicação nesta coleção
13 Ago 2010
Data do Fascículo
1988

Histórico

Recebido
10 Nov 1988
Aceito
22 Mar 1989

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ACHITUV, M. & BAR-AKIVA, A. Glutamic-oxaloacetic transamine in leaves of phosphorus deficient citrus leaves. Physiologia Plantarum, Copenhagen, 36:368-73 , 1976.

[2] ACHITUV, M. & BAR-AKIVA, A. Nitrogen accumulation induced by phosphorus deficiency in citrus plants. Scientia Horticulturae, Amsterdam, 1:251-62, 1973.

[3] BASSO, L.C. Significado bioquímico da deficięncia de potássio na formaçăo de aminas em gergelim (Sesamum indicum) Botucatu, 1973. 85p. (Doutorado - Faculdade de Cięncias Médicas e Biológicas de Botucatu).

[4] BASSO, L.C. & SMITH, T.A. Effect of mineral deficiency on amine formation in higher plants. Phytochemistry, Oxford, 13:875-83, 1974.

[5] BESFORD, R.T. & HOBSON, G.E. Effect of potassium nutrition on some enxymes from ripening Lycopersicon esculetun fruit. Phytochemistry, Oxford, 14:57-99,1975.

[6] BROWN, G.W. & COHEN, P.P. Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver. Journal of Biological Chemistry, Baltimore, 234:1769-74, 1959.

[7] CATTINI, M.A. Contribuiçăo ao estudo do papel fisiológico e bioquímico do potássio em plantas de feijăo (Phaseolus vulgaris L. cv. Goiano precoce). Piracicaba, 1974. 67p. (Mestrado - Escola Superior de Agricultura "Luiz de Queiroz"/USP).

[8] COBRA NETTO, A.

[9] CROCOMO, O.J. & BASSO, L.C. Accumulation of putrescine and related amino acids in potassium deficient Sesamum. Phytochemistry, Oxford, 13:2659-65, 1974.

[10] CROCOMO, O.J.; CATTINI, M.A.; ZAGO, E.A. Acumulo de aminas e aminoácidos em relaçăo ao nível de potássio em folhas de feijăo (Phaseolus vulgaris L.). Arquivos de Biologia e Tecnologia, Curitiba, 17:93-102, 1974.

[11] EATON, S.A. Effects of potassium deficiency on growth and metabolism of sunflower plants. Botanical Gazette, Chicago, 114:165-80, 1952.

[12] ELLIS, R.J. & DAVIES, D.D. Clutamic-oxaloacetic transaminase of cauliflower. I, Purification ans specificity. Biochemical Journal, London, 78:615-23, 1961.

[13] GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilaçăo do nitrogęnio afetada pela deficięncia de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). Săo Paulo, 1977. 108p.(Doutorado - Instituto de Química/USP).

[14] GUTIERREZ, L.E.; CROCOMO, O.J.; BASSO, L.C. Efeito da deficięncia de potássio sobre alguns aspectos metabólicos em feijoeiro (Phaseolus vulgaris L.). Revista de Agricultura, Piracicaba, 53(4):209-16, 1978.

[15] HARPER, J.E. & PAULSEN, G.M. Nitrogen assimilation and protein synthesis in wheat seedlings as affected by mineral nutrition. I. Macronutrients. Plant Physiology, Lancaster, 44:69-74, 1969.

[16] KASTING, R. & DELWICHE,C.C. Orinithine, citrulline and arginine metabolism in water melon seedlings. Plant Physiology, Lancaster, 33:350-4, 1958.

[17] KLECZOWSKI, K. & COHEN, P.P. Purification of ornithine transcarbamylase from pea seed lings. Archives of Biochemistry and Biophysics, New York, 107:211-8 , 1964.

[18] LOWRY, O.H.; ROSEBROYGH, N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193:265 -75, 1951.

[19] MALLERY, C.H. Isoenzyme activities during the early stages of Allium radicle germination. Physiologia Plantarum, Copenhagen, 26:136-42, 1972.

[20] MATSUM0T0, H. WAKIUCHI, N. Changes in ATP in cucumber leaves during ammonium toxicity. Zeitschrift fur Pflanzenphysiologie, Jena, 73:82-5, 1974. Apud Chemical Abstracts, Easton, 81:115-468y.

[21] PATNAIK, R.; BARKER, A.V.; MAYNARD, D.N. Effects of ammonium and potassium ions on some physiological and biochemical process of excised cucumber cotyledons. Physiologia Plantarum, Copenhagen, 27:32-6, 1972.

[22] PIMENTEL GOMES, F. Curso de estatística experimental. 4.ed. Săo Paulo, 1970. 430p.

[23] SMITH, T.A. The occurrence, metabolism and functions of amines in plants. Biological Review of the Cambridge Philosophical Society, Cambridge, 46:201-41, 1971.

[24] SPLITTSTOESSER, W.E. Metabolism of arginine by aging in 7 days old punpkin seedlings. Plant Physiology , Lancaster, 44:361, 1969.

[25] SPLITTSTOESSER, W.E. & STEWART, S.A. Distribution and isoenzymes of aspartate aminotransferase in cotyledons of germinating punpkins. Physiologia Plantarum, Copenhagen, 23:1119-26, 1970.

[26] WAKIUCHI, N.; MATSUMOTO, H.; TAKAHASHI, E. Changes of some enzymes activities of Cucumber during ammonium toxicity. Physiologia Plantarum, Copenhagen, 24:248-51, 1971.

[27] WONG, K.F. & COSSINS, E.A. Studies of the particulate and soluble aspartate amino transferase in germinating pea cotyledons. Phytochemistry, Oxford , 8:1327-38, 1969.

Brasil

Brasil

Atividades de transaminase glutamico-oxaloacético e transcarbamilase de ornitina em folhas de feijoeiro (Phaseolus vulgaris L.) afetadas pela deficiência de potássio

Ornithine transcarbamylase and glutamic-oxaloacetic transaminase activities of bean leaves (Phaseolus vulgaris L.) as affected by potassium deficiency

Resumos

Datas de Publicação

Histórico