Acúmulo de trealose em linhagens de Saccharomyces durante fermentação alcoólica

Gutierrez, L.E.

doi:10.1590/S0071-12761990000200018

Resumos

A pesquisa foi realizada para comparar os efeitos de diversos fatores (temperatura, pH, concentração de sacarose, 2,4-dinitrofenol e fontes de nitrogênio) sobre a produção de trealose em Saccharomyces uvarum IZ-1904 e Saccharomyces cerevisiae (M-300-A e de panificaçao) durante a fermentação alcoólica. Com a levedura IZ-1904 houve menor produção de trealose do que M-300-A e de panificaçao. A trealose foi formada em maior quantidade (p < 0,05) a 34°C. Em pH 4,5 houve maior acúmulo de trealose do que em pH 3,0 para as leveduras M-300-A e de panificaçao. A adição de 18ppm de 2,4-dinitrofenol acarretou decréscimo (p < 0,05) na quantidade de trealose formada pela levedura de panificaçao e sem efeito para IZ-1904. O aumento da concentração de sacarose ocasionou a maior produção de trealose.

trealose; Saccharomyces; fermentação alcoólica

This study was carried out to compare the effects of several factors (temperature, pH, sucrose concentration, 2,4-dinitrophenol and nitrogen sources) on trehalose production by Saccharomyces uvarum IZ-1904 and Saccharomyces cerevisiae (M-300-A and baker's yeast) during alcoholic fermentation. Trehalose production was lower with the yeast LZ-1904 than with M-300-A or baker's yeast. More trehalose was formed at 34°C than at 25°C. For M-300-A and baker's yeast trehalose accumulation was higher at pH 4.5 than at pH 3.0. Addition of 18ppm 2.4-dinitrophenol strongly decreased the amount of trehalose formed by baker's yeast, but was ineffective for IZ-1904. Increase of sucrose concentration led to a higher production of trehalose.

Trehalose; Saccharomyces; alcoholic fermentation

ARTIGOS ARTICLES

TECNOLOGIA AGROINDUSTRIAL AGROINDUSTRIAL TECNOLOGY

Acúmulo de trealose em linhagens de Saccharomyces durante fermentação alcoólica

Trehalose accumulation of strains of Saccharomyces during alcoholic fermentation

L.E. Gutierrez

Departamento de Química da E.S.A. "Luiz de Queiroz" da Universidade de São Paulo - 13.400 - Piracicaba, SP e CEBTEC/FEALQ

RESUMO

A pesquisa foi realizada para comparar os efeitos de diversos fatores (temperatura, pH, concentração de sacarose, 2,4-dinitrofenol e fontes de nitrogênio) sobre a produção de trealose em Saccharomyces uvarum IZ-1904 e Saccharomyces cerevisiae (M-300-A e de panificaçao) durante a fermentação alcoólica. Com a levedura IZ-1904 houve menor produção de trealose do que M-300-A e de panificaçao. A trealose foi formada em maior quantidade (p < 0,05) a 34°C. Em pH 4,5 houve maior acúmulo de trealose do que em pH 3,0 para as leveduras M-300-A e de panificaçao. A adição de 18ppm de 2,4-dinitrofenol acarretou decréscimo (p < 0,05) na quantidade de trealose formada pela levedura de panificaçao e sem efeito para IZ-1904. O aumento da concentração de sacarose ocasionou a maior produção de trealose.

Termos para indexação: trealose, Saccharomyces, fermentação alcoólica.

ABSTRACT

This study was carried out to compare the effects of several factors (temperature, pH, sucrose concentration, 2,4-dinitrophenol and nitrogen sources) on trehalose production by Saccharomyces uvarum IZ-1904 and Saccharomyces cerevisiae (M-300-A and baker's yeast) during alcoholic fermentation. Trehalose production was lower with the yeast LZ-1904 than with M-300-A or baker's yeast. More trehalose was formed at 34°C than at 25°C. For M-300-A and baker's yeast trehalose accumulation was higher at pH 4.5 than at pH 3.0. Addition of 18ppm 2.4-dinitrophenol strongly decreased the amount of trehalose formed by baker's yeast, but was ineffective for IZ-1904. Increase of sucrose concentration led to a higher production of trehalose.

Index terms: Trehalose, Saccharomyces, alcoholic fermentation.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Entregue para publicação em: 27/08/90

Aprovado para publicação em: 20/12/90

AMIN, G.; STANDAERT, P.; VERACHTERT, H. Effects of metabolic inhibitors on the alcoholic fermentation by several yeasts in batch or in immobilized cell systems. Applied Microbiology and Biotechnology, Berlim, 19:91-9, 1984.
BERKE, H.L. & ROTHSTEIN, A. The metabolism of storage carbohydrates in yeast, studied with glucose-1-C¹⁴ and dinitrophenol. Archives of Biochemistry and Biophysics, New York, 72:380-95, 1957.
BRADY, T.G.; DUGGAN, P.F.; McGANN, C.; TULLY, E. Study of the endogenous fermentation induced in baker's yeast by azide, 2,4-dinitrophenol and arsenite.Archives of Biochemistry and Biophysics, New York, 93:220-30, 1961.
CHESTER, V.E.; BYRNE, M.J. Carbohydrate composition and UDP-glucose concentration in a normal yeast and a mutant deficient in glycogen. Archives of Biochemistry and Biophysics, New York, 127:556-62, 1968.
DUNTZE, W.; NEUMANN, D.; GANCEDO, J.; ATZPODIEN, W.; HOLZER, H. Regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. European Journal of Biochemistry, Berlin, 10(1): 83-9, 1969.
GOMES, F.P. Curso de Estatística Experimental, São Paulo, Nobel, 1970. 485p.
GRBA, S.; OURA, E.; SUOMALAINEM, H. On the formation of glycogen and trehalose in baker's yeast. European Journal of Applied Microbiology, Berlin, 2:29-37, 1975.
GUTIERREZ, L.E. Estudo comparativo de fermentação alcoólica por linhagens de Saccharomyces cerevisiae e Saccharomyces uvarum. Piracicaba, 1989. 160p. (Livre-docência - Escola Superior de Agricultura "Luiz de Queiroz"/USP) .
KUENZI, M.T. & FIECHTER, A. Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Archives of Microbiology, Berlin, 84:254-65, 1972.
LILLIE, S.H. & PRINGLE, J.R. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: response to nutrient limitation. Journal of Bacteriology, Baltimore, 143:1384-94, 1980.
MACKENZIE, K.F.; SINGH, K.K.; BROWN, A.D. Water stress plating hypersensitivity of yeasts; protective role of trehalose in Saccharomyces cerevisiae. Journal of General Microbiology, London, 134(6):1661-6, 1988.
PANEK, A.D. Synthesis of trehalose by baker's yeast (Saccharomyces cerevisiae).Archives of Biochemistry and Biophysics, New York, 98:349-65, 1962.
PANEK, A.D. Energy requirements for trehalose synthesis. Ciência, e Cultura, São Paulo, 23(1): 75-9, 1971.
PANEK, A.D. Metabolismo de trealose em Saccharomyces cerevisiae. Rio de Janeiro, 1975. 115p. (Doutorado - Instituto de Química/Universidade Federal do Rio de Janeiro).
PARADA, G. & ACEVEDO, F. On the relation of temperature and RNA content to the specific growth rate inSaccharomyces cerevisiae. Biotechnology and Bioengineering, New York, 25:2785-8, 1983.
PULLMAN, M.E.; PENEFSKY, H.S.; DATTA, A.; RACKER, G. Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphate. Journal of Biological Chemistry, Baltimore, 235(11):3322-9, 1960.
SUOMALAINEN, H. & PFAFFLI, S. Changes in the carbohydrate reserves of baker's yeast during growth and on standing. Journal of the Institute of Brewing, London, 67:249-54, 1961.
THEVELEIN, J.M. Regulation of trehalose mobilization in fungi. Microbiological Reviews, Washington, 48(1):42-59, 1984.
TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism 5. The trehalose content of baker's yeast during anaerobic fermentation. BiochemicalJournal, London, 62:177-83, 1956a.
TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism. 6. Effects of thiamine on nitrogen assimilation during fermentation and on fermentation kinetics. Biochemical Journal, London, 62:183-90, 1956b.
TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism. 7. Yeast carbohydrate fraction. Separation from nucleic acid, analysis and behaviour during anaerobic fermentation. Biochemical Journal, London, 63:23-33, 1956c.

Datas de Publicação

Publicação nesta coleção
06 Maio 2009
Data do Fascículo
1990

Histórico

Aceito
20 Dez 1990
Recebido
27 Ago 1990

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] AMIN, G.; STANDAERT, P.; VERACHTERT, H. Effects of metabolic inhibitors on the alcoholic fermentation by several yeasts in batch or in immobilized cell systems. Applied Microbiology and Biotechnology, Berlim, 19:91-9, 1984.

[2] BERKE, H.L. & ROTHSTEIN, A. The metabolism of storage carbohydrates in yeast, studied with glucose-1-C¹⁴ and dinitrophenol. Archives of Biochemistry and Biophysics, New York, 72:380-95, 1957.

[3] BRADY, T.G.; DUGGAN, P.F.; McGANN, C.; TULLY, E. Study of the endogenous fermentation induced in baker's yeast by azide, 2,4-dinitrophenol and arsenite.Archives of Biochemistry and Biophysics, New York, 93:220-30, 1961.

[4] CHESTER, V.E.; BYRNE, M.J. Carbohydrate composition and UDP-glucose concentration in a normal yeast and a mutant deficient in glycogen. Archives of Biochemistry and Biophysics, New York, 127:556-62, 1968.

[5] DUNTZE, W.; NEUMANN, D.; GANCEDO, J.; ATZPODIEN, W.; HOLZER, H. Regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. European Journal of Biochemistry, Berlin, 10(1): 83-9, 1969.

[6] GOMES, F.P. Curso de Estatística Experimental, São Paulo, Nobel, 1970. 485p.

[7] GRBA, S.; OURA, E.; SUOMALAINEM, H. On the formation of glycogen and trehalose in baker's yeast. European Journal of Applied Microbiology, Berlin, 2:29-37, 1975.

[8] GUTIERREZ, L.E. Estudo comparativo de fermentação alcoólica por linhagens de Saccharomyces cerevisiae e Saccharomyces uvarum. Piracicaba, 1989. 160p. (Livre-docência - Escola Superior de Agricultura "Luiz de Queiroz"/USP) .

[9] KUENZI, M.T. & FIECHTER, A. Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Archives of Microbiology, Berlin, 84:254-65, 1972.

[10] LILLIE, S.H. & PRINGLE, J.R. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: response to nutrient limitation. Journal of Bacteriology, Baltimore, 143:1384-94, 1980.

[11] MACKENZIE, K.F.; SINGH, K.K.; BROWN, A.D. Water stress plating hypersensitivity of yeasts; protective role of trehalose in Saccharomyces cerevisiae. Journal of General Microbiology, London, 134(6):1661-6, 1988.

[12] PANEK, A.D. Synthesis of trehalose by baker's yeast (Saccharomyces cerevisiae).Archives of Biochemistry and Biophysics, New York, 98:349-65, 1962.

[13] PANEK, A.D. Energy requirements for trehalose synthesis. Ciência, e Cultura, São Paulo, 23(1): 75-9, 1971.

[14] PANEK, A.D. Metabolismo de trealose em Saccharomyces cerevisiae. Rio de Janeiro, 1975. 115p. (Doutorado - Instituto de Química/Universidade Federal do Rio de Janeiro).

[15] PARADA, G. & ACEVEDO, F. On the relation of temperature and RNA content to the specific growth rate inSaccharomyces cerevisiae. Biotechnology and Bioengineering, New York, 25:2785-8, 1983.

[16] PULLMAN, M.E.; PENEFSKY, H.S.; DATTA, A.; RACKER, G. Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphate. Journal of Biological Chemistry, Baltimore, 235(11):3322-9, 1960.

[17] SUOMALAINEN, H. & PFAFFLI, S. Changes in the carbohydrate reserves of baker's yeast during growth and on standing. Journal of the Institute of Brewing, London, 67:249-54, 1961.

[18] THEVELEIN, J.M. Regulation of trehalose mobilization in fungi. Microbiological Reviews, Washington, 48(1):42-59, 1984.

[19] TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism 5. The trehalose content of baker's yeast during anaerobic fermentation. BiochemicalJournal, London, 62:177-83, 1956a.

[20] TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism. 6. Effects of thiamine on nitrogen assimilation during fermentation and on fermentation kinetics. Biochemical Journal, London, 62:183-90, 1956b.

[21] TREVELYAN, W.E. & HARRISON, J.S. Studies on yeast metabolism. 7. Yeast carbohydrate fraction. Separation from nucleic acid, analysis and behaviour during anaerobic fermentation. Biochemical Journal, London, 63:23-33, 1956c.

Brasil

Brasil

Acúmulo de trealose em linhagens de Saccharomyces durante fermentação alcoólica

Trehalose accumulation of strains of Saccharomyces during alcoholic fermentation

Resumos

Datas de Publicação

Histórico