Age and growth parameters of cachara Pseudoplastystoma reticulatum (Siluriformes, Pimelodidae) from the Cuiabá River, Brazil

Idade e parâmetros de crescimento do cachara Pseudoplastystoma reticulatum (Siluriformes, Pimelodidae) do Rio Cuiabá, Brasil

Age and growth parameters of cachara Pseudoplastystoma reticulatum (Siluriformes, Pimelodidae) from the Cuiabá River, Brazil

Idade e parâmetros de crescimento do cachara Pseudoplastystoma reticulatum (Siluriformes, Pimelodidae) do Rio Cuiabá, Brasil

Talitha M. Francisco^I; Angela Maria Ambrósio^I; Thiago José Balbi^I; Marina S. Zuliani^I; Edson K. Okada^I; Luiz C. Gomes^I,II

^INúcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura - Nupélia, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil. ([e-mail])

^IICurso de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Departamento de Biologia, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil

ABSTRACT

Age and growth parameters of cachara Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889) (Siluriformes,

Pimelodidae) (males and females) were estimated through the analysis of growth rings in spines of pectoral fins. Fish were collected from January to December 2007, in the area directly influenced by the Aproveitamento Múltiplo de Manso (APM Manso) and in the Cuiabá River (upper parts of the Pantanal). The maximum number of growth rings was seven for males, and eight, for females. The analysis of temporal variations in mean marginal increment showed that rings found in the spines were formed annually, in December. Growth rings were associated to spawning (in the study region from November to March) of the species. The growth curve in length was obtained by the von Bertalanffy model adjusted by the Ford-Walford transformation. The equations are: Ls = 72.7*[1-e^{-0.44(t+1.5974)}] for males, and Ls = 84.5*[1-e^{-0.33(t+2.0943)}] for females. The equations that describe the growth curve in weight are: Wt = 4991.61*[1-e^{-0.44 (t+1.5974}] ^2.70 for males and Wt = 7503.17*[1-e^{-0.33 (t+2.0943}] ^2.99 for females.

Keywords: Growth rings, pectoral fins, von Bertalanffy model, Ford-Walford.

RESUMO

A idade e os parâmetros de crescimento de machos e fêmeas do cachara, Pseudoplastystoma reticulatum (Eigenmann & Eigenmann, 1889) (Siluriformes, Pimelodidae), coletados de janeiro a dezembro de 2007, na área de influencia do Aproveitamento Múltiplo de Manso (APM Manso) e rio Cuiabá (parte superior do pantanal), foram estimados através das marcas de aposição (anéis) observadas nos acúleos das nadadeiras peitorais. O número máximo de anéis encontrados foi sete para os machos e oito para as fêmeas. Os anéis encontrados nos acúleos foram anuais e formados em dezembro, como observado através da análise da variação temporal do incremento marginal médio. Possivelmente estes estão relacionados com a época de reprodução para a espécie na área (novembro a março). A curva de crescimento em comprimento foi obtida pelo modelo de von Bertalanffy, ajustada pela transformação Ford-Walford. As equações são: Cp = 72,7*[1-e^{-0,44(t+1,5974)}] para machos e Cp = 84,5*[1-e^{-0,33(t+2,0943)}] para fêmeas. As equações que descrevem a curva de crescimento em peso são: Pt = 4991,61*[1-e^{-0,44(t+1,5974)}]^2,70 para machos e Pt = 7503,17*[1-e^{-0,33(t+2,0943)}]^2,99 para fêmeas.

Palavras-chave: Marcas de aposição, nadadeira peitoral, modelo de von Bertalanffy, Ford-Walford.

Studies on population dynamics are essential to improve knowledge on the biology of fish species (Nikolsky, 1969). Therefore, the determination of age from calcified structures of fish is a vital component of most present-day fisheries management decisions (Campana, 2001), despite the difficulties to study growth, particularly in tropical regions (Menon, 1953; Ferreira & russ 1994), where fish diversity is high.

This high number of species, including in the Neotropical region, allows fisheries to be conducted on several species, especially Characiformes and Siluriformes (Petrere & Agostinho, 1993; Mateus et al., 2004). These authors also showed that among the seven species of large Siluriformes (total length > 50 cm) of the Pantanal, four of them -Pseudoplatystoma corruscans (Spix & Agassiz, 1829), Pseudoplastystoma reticulatum (Eigenmann & Eigenmann, 1889), Pinirampus pirinampu (Agassiz, 1829) and Zungaro jahu (Ihering, 1898) - represented 64% of the total catch in the Cuiabá city in 2000 and 2001. albuquerque et al. (2003) and Silva (1986) also reported a great capture of P. reticulatum and P. corruscans in the same region. The great economic importance of P. reticulatum in the area studied is also discussed by Resende et al. (1996) and Tavares (1997).

Pseudoplastystoma reticulatum is known in the region as cachara, surubim and surubim-cachara, with nocturnal habits and considered a piscivorous species (Resende et al., 1996). Taking into account the importance of P. reticulatum as a fishery resource in the area (Oliveira et al., 2005), this paper aims to determine the age and growth parameters of this species, a fundamental information for stock assessment. In addition, the periodicity in ring formation (monthly means of marginal increment) was analyzed and in order to validate it, we used the coefficient of variation of mean standard length for each age class. Thus, the von Bertalanffy model was adjusted to estimate the growth parameters for each sex. Finally, the results are discussed in relation to the biology of the species and some inferences are made on the effect of the fishery on the age structure of cachara.

MATERIAL AND METHODS

The Cuiabá River basin is located in the 'Cuiabana' depression, plains and 'pantanais' in the state of Mato Grosso. In the upper part of the studied area, is situated the APM Manso Reservoir (parallels 14º32' - 15º40'S and 54º40' - 55º55'W) (Fig. 1). This was filled in 1999 and flooded a stretch of the Manso River and the lower sections of Casca, Palmeiras and Quilombo rivers. Downstream of the APM Manso Reservoir, the Manso River joins the Cuiabazinho River, forming the Cuiabá River. The Cuiabá River is the main tributary of the Paraguay River and forms the upper part of the 'Pantanal matogrossense' near the cities of Cuiabá and Várzea Grande (Oliveira et al., 2005).

Individuals of P. reticulatum were obtained monthly from January to December 2007 from the landings of the artisanal (professional) fisheries conducted in the region. In this region, gill nets are forbidden for fishing. Then, fishers employ several gears with hooks, such as long lines, rods, and line and hooks attached to branches (attached to trees in the margins). Every individual was measured (standard length, Ls; cm) and weighed (total weight, Wt; g). Sex was identified and the spines of the pectoral fins were removed. After removal, spines were cleaned, dried, cut and mounted between glass slides for reading, following Reina et al. (1995).

Identification of rings (annuli) in the spines was conducted under stereoscopic microscope, using a micrometer ocular. For each spine we measured the radius (mm), which is the distance between the center of the spine (focus or nucleus) and its anterior margin, and the distance from the center of the spine to each annulus (mm). We conducted three independent readings of all spines, and in case of discordance in the number of growth rings between readings, the spine was discarded or replaced.

To determine the timing and periodicity of ring formation, we calculated monthly means of the marginal increment, using the expression presented in lai et al. (1996): M.I.= (R - r_i)/(r_i-r_i-1), where R = centrum-radii, the distance between the center of the spine (focus or nucleus) and its anterior margin; r_i = ultimate annulusradii (the distance between the center of the spine and the ultimate annulus); and r_i-1= penultimate annuli-radii (the distance between the center of the spine and the penultimate annulus).

Validation of the growth rings found in spines was performed through the analysis of the coefficient of variations (CVs) for the standard mean lengths for each growth ring (lai et al., 1996), for each sex separately.

The model selected to express the growth of P. reticulatum was the von Bertalanffy, adjusted using Ford-Walford transformation (WalFord, 1946). This transformation was selected because a nonlinear procedure may result in incorrect growth parameters (unreal or inconsistent). After estimating the parameters k (growth rate) and Ls (asymptotic length), it was possible to determine the growth curve in length, described by the expression: Ls = Ls_*[1-e^{-k(t- t0)}], where Ls = standard length of the individuals with age "t" (cm); Ls = maximum standard (asymptotic) length that individuals can achieve (cm); e = Napierian logarithm; k = parameter related to growth rate (year^-1); t = age of individuals (years) and t0 = age at which the organism would have had size equal zero. The parameter t₀ was estimated using the expression presented in vazzoler (1981). We also obtained the standard length vs. total weight relationship for each sex separately, using the nonlinear estimation and Levenberg-Marquardt method. The expression (le CreN, 1951) used was: Wt = a*Ls^b, where Wt = total weight (g); Ls = standard length (cm); a = intercept and b = slope.

After estimated the parameters of the length x weight relationship, we can determine the asymptotic weight (Wt), solving this relation for Ls. This allows determining the growth in weight using the direct method as in Ambrósio et al. (2003), described by the expression: Wt = Wt_*[1-e^{-k (t-t0)}]^b, where Wt = weight of individuals with age "t" (g); Wt = maximum total (asymptotic) weight that individuals can achieve (g); e = Napierian logarithm; k = parameter related to growth rate (year^-1); t = age of individuals (years); t₀ = age at which the organism would have had size equal zero, and b = slope of the relationship between total weight and standard length. Since females may present larger body size (Wootton, 1998), models were fit for each sex separately.

RESULTS

In this study, 250 individuals of P. reticulatum (146 males and 104 females) were examined. For males, the smallest individual had 40.5 cm long and the largest had 85.0 cm. The smallest female had 46.0 cm long, and the largest, 96.0 cm. The number of rings varied from one to seven for males, and from one to eight for females; but most fish (of both sexes) presented three to five growth rings, showing that the population in the region presents few old individuals. The formation of the ring occurred in December when the mean monthly marginal increment was lower (Fig. 2).

The method selected to validate the rings observed in the spines of P. reticulatum was the coefficient of variation (CV) of the standard length of all fish with the same number of rings, separated by sex (Tab. I). Overall, for this study, CV were low (below 20%), indicating a good consistence in the readings.

The equations that describe the growth in length for the species are: Ls = 72.7*[1-e^{-0.44 (t+1.5974)}] (males) and Ls = 82.5*[1-e^{-0.33 (t+2.9043)}] (females). Females presented higher asymptotic length (L = 82.5 cm) than males (L = 72.7 cm). The constant of growth was greater for the males (k = 0.44) than for females (k = 0.33), indicating that males grow faster (Fig. 3).

The relationship between standard length and total weight, for each sex (Fig. 4), was expressed by the following equations: Wt = 0.047*Ls^2.70 (R² = 0.89) (males) and Wt= 0.013*Ls^2.99 (R² = 0.91) (females). The confidence intervals to the constants a and b were, respectively: 0.012-0.082 and 2.52-2.87 (95% level of confidence) for males and 0.0007-0.025 and 2.78-3.21 for females (95% level of confidence). Thus, the equations that describe the growth in weight are: Wt 4991.61*[1-e^{-0.44*(t+1.5974)}]^2.70 = for males; and Wt = 7503.17*[1-e^{-0.33*(t+2.0943)}]^2.99 for females.

DISCUSSION

Validation of rings in studies of age and growth is important and necessary to obtain unbiased parameters for the von Bertalanffy equation (Casselman, 1983; Beamish & McFarlane, 1983; Cyterski & Spangler, 1996; Ambrósio & Hayashi, 1997; Jepsen et al., 1999; Ambrósio et al., 2003). This is especially true in regions where climate is less variable, which leads to weak apposition marks (Ambrósio & Hayashi, 1997; Ambrósio et al., 2003). In addition, the process of estimating age incorporates biases associated with the non-formation of rings as fish grow, and these biases can lead to under-or overestimated ages (Campana, 2001), both reflecting in the quality of the von Bertalanffy equation parameters. Another common error, according to this author, is related to the subjectivity in the estimates of age or the accuracy (interpretation of the numbers of rings) and precision (number of fish studied). This subjectivity arises during samplings (collection of fish), and may increase with preparation of material and interpretation of the growth marks (readings).

Formation of only one growth ring along a year is common in tropical regions (Reina et al., 1995; Ambrósio & Hayashi, 1997). However, to determine the timing and factor influencing the formation of growth rings in Neotropical fish is still a challenge. The formation of those rings in fish of the Neotropical region has been usually associated with spawning (Barbieri, 1989; Castro, 1998; Barbieri et al., 2000; Feitosa et al., 2004), especially in the studied region where annual mean temperature ranges from 21.4ºC to 27.4ºC (Girard et al., 2003).

Ambrósio et al. (2003) affirm that the most important mechanism affecting the growth rings' formation in fish from tropical regions is the reproductive process. During this process, several species may present suspension or reduction in feeding, and the growth of individuals decreases.

However, other aspects may be related to reproduction, such as maturation, migration, spawning, and parental care, exogenous factors frequently related to the formation of rings in calcified structures of tropical fish (Dudley, 1974; Fagade, 1974; Barbieri & Barbieri, 1983; Santos & Barbieri, 1991). In the case of P. reticulatum, we observed that its reproduction in the region starts in November extending until March, period when the monthly means of the marginal increment are lower; therefore the growth rings seem to be related to the reproduction of the species.

Females of P. reticulatum presented higher growth rate than males and achieved longer length and weight, following the general pattern registered for fish (Wootton, 1998). The values of the parameter k estimated in this study were high (0.44 and 0.33 for males and females respectively). High values of k were also found for Salminus brasiliensis (Cuvier, 1817) in the upper Paraná River floodplain (0.28 for males and 0.26 for females) (Feitosa et al., 2004), and Corumbá Reservoir (0.77 for males and 0.52 for females) (dei tos et al. 2009). Despite being a Characiformes species, Salminus brasiliensis also migrates long distances like the Siluriformes P. reticulatum, and both species are classified as having the periodic strategy (WiNemiller, 1989). These species maximize fecundity and are well suited to determine spatiotemporal variability.

Few studies have been conducted to estimate growth of fish in Neotropical region. Thus, this study represents the first to determine the age and growth parameter in Cuiabá River. The consistency of the readings was high, with coefficient of variation of the length, for each growth ring, lower than 17% for both sexes. The formation of the growth rings occurred in December, probably related to the reproduction of the species in the region, which extends from November to March. At last, the asymptotic length was within the range found in the literature.

Acknowledgements. The authors thank Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia) for the logistic support during samplings and analyses of biological material. FURNAS Centrais Elétricas funded the study. LCG is researcher of CNPq (Productivity Scholarship).

Recebido em 9 de fevereiro de 2011.

Aceito em 29 de dezembro de 2011.

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Publication Dates

• Publication in this collection
  08 Mar 2012
• Date of issue
  Dec 2011

History

• Accepted
  29 Dec 2011
• Received
  09 Feb 2011