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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.77 no.1 Rio de Janeiro Jan./Mar. 1982 

Manutenção em laboratório de Anopheles albitarsis e Anopheles aquasalis por copulacao induzida

Mercia E. Arruda1 

Ricardo I. Rios2 

Paulo C. Arantes2 

Ana C. Oliveira2 

Luzenário P. Nascimento2 

Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

UFRJ, Instituto de Biologia, Rio de Janeiro, Brasil


Introduzimos a técnica da copulação induzida para a manutenção e criação em cativeiro, do Anopheles albitarsis e do A. aquasalis, objetivando facilitar futuros trabalhoscom estas espécies de difícil cruzamento espontâneo em laboratório.


Many species of mosquitoes are unsuited to life in a laboratory because they fail to copulate in cages. This situation may be largely eliminated by the technique in current use for manually inducing inseminations as outlined. We have used this technique for the first time in relation to Anopheles albitarsis and A. aquasalis, species of malaria vectors which are difficult to mate spontaneously in captivity. The pre-mating age of the males and the temperature at which they are held affect the ability of male to transfer sperm. Mating males 8 to 14 days old with females at any age, resulted in a high order of insemination. Temperature of 25°C and humidity of 80% RH enhanced the degree of insemination.


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