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vol.82 suppl.4Genomic regulation in imature females of Schistosoma mansoniSchistosomiasis mansoni: immunodiagnosis aspects and search for an immunological marker related to therapeutic efficacy author indexsubject indexarticles search
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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.82  suppl.4 Rio de Janeiro  1987

https://doi.org/10.1590/S0074-02761987000800037 

Decay accelerating factor (DAF) as the host antigen with protective activity to complement killing of schistosomula

F. Juarez Ramalho-Pinto1 

UFMG, Departamento de Bioquímica e Imunologia, Belo Horizonte, Brasil


ABSTRACT

The acquisition of host antigens by Schistosoma mansoni was studied by evaluating the resistance of schistosomula to the complement attack mediated by lethal antibody. Schistosomula cultured for 24 hours with intact human erythrocytes (N-HuE) or ghosts of any type of ABO or Rh blood group, showed a marked resistance to complement damage. Sheep red blood cells, pronase-treated N-HuE or erythrocytes from patients with paroxysmal nocturnal hemoglobinuria, which are complement-sensitive cells, were unable to protect schistosomula. Schistosomula protected by N-HuE became again susceptible to complement killing after incubation with a monoclonal antibody anti-DAF. These results indicate that, in vitro, host DAF from N-HuE can be acquired by schistosomula surface in a biological active form that protects the parasite from the complement lesion.

 

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