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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.85 no.1 Rio de Janeiro Jan./Mar. 1990

http://dx.doi.org/10.1590/S0074-02761990000100018 

Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures

Frederico Guilherme Coutinho Abath1 

Alzira Maria Paiva de Almeida1 

Luís Carlos de Souza Ferreira2 

FIOCRUZ, Centro de Pesquisas Aggeu Magalhães, Recife, Brasil

Laboratório de Imunopatologia Keizo Asami, Departamento de Biofísica e Radiologia, Recife, Brasil

ABSTRACT

Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.

Key words: Yersinia pestis; outer membrame proteins

 

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