Services on Demand
Journal
Article
English (pdf)
Article in xml format
Curriculum ScienTI
Automatic translationIndicators
-
Cited by SciELO -
Access statistics
Related links
Cited by Google -
Similars in
SciELO 
Similars in Google 
uBio
Share
Permalink
Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276On-line version ISSN 1678-8060
Mem. Inst. Oswaldo Cruz vol.85 no.1 Rio de Janeiro Jan./Mar. 1990
http://dx.doi.org/10.1590/S0074-02761990000100018
Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
FIOCRUZ, Centro de Pesquisas Aggeu Magalhães, Recife, Brasil
Laboratório de Imunopatologia Keizo Asami, Departamento de Biofísica e Radiologia, Recife, Brasil
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
Key words: Yersinia pestis; outer membrame proteins
