Abstract
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
Yersinia pestis; outer membrame proteins
Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
Frederico Guilherme Coutinho Abath1
Alzira Maria Paiva de Almeida1
Luís Carlos de Souza Ferreira2
FIOCRUZ, Centro de Pesquisas Aggeu Magalhães, Recife, Brasil
Laboratório de Imunopatologia Keizo Asami, Departamento de Biofísica e Radiologia, Recife, Brasil
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
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Publication Dates
-
Publication in this collection
24 June 2009 -
Date of issue
Mar 1990