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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.85 no.3 Rio de Janeiro July./Sept. 1990 

Serological diagnosis of dengue by an Elisa inhibition method (EIM)

R. J. Fernandez1 

S. Vazquez1 

Instituto de Medicina Tropical Pedro Kouri, Departamento de Virologia, Laboratório de Arbovírus, La Habana, Cuba


An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.


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