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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276

Mem. Inst. Oswaldo Cruz v.85 n.3 Rio de Janeiro jul./sept. 1990

http://dx.doi.org/10.1590/S0074-02761990000300012 

Serological diagnosis of dengue by an Elisa inhibition method (EIM)

R. J. Fernandez, S. Vazquez

Instituto de Medicina Tropical Pedro Kouri, Departamento de Virologia, Laboratório de Arbovírus, Cuba

ABSTRACT

An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.