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Freeze-fracture study of Trichomonas vaginalis

Abstract

The freeze-fracture technique was used to analyse the organization of the plasma membrane, as well as membranes of cytoplasmic organelles, of the pathogenic protozoan Trichomonas vaginalis. Rosettes formed by 4 to 14 intramembranous particles were seen on the fracture faces of the membrane lining the anterior flagella as well as in fracture faces of the plasma membrane enclosing the anterior region of the protozoan and in cytoplasmic organelles. Special organization of the membrane particles were also seen in the region of association of the recurrent flagellum to the cell body.

Trichomonas vaginalis; membrane structure; freeze-fracture; electron microscopy


ABSTRACT

Freeze-fracture study of Trichomonas vaginalis

Marlene Benchimol1

Wanderley de Souza

Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Departamento de Parasitologia e Biofísica. laboratório de Ultraestrutura Celular e Microscopia, Rio de Janeiro, Brasil

The freeze-fracture technique was used to analyse the organization of the plasma membrane, as well as membranes of cytoplasmic organelles, of the pathogenic protozoan Trichomonas vaginalis. Rosettes formed by 4 to 14 intramembranous particles were seen on the fracture faces of the membrane lining the anterior flagella as well as in fracture faces of the plasma membrane enclosing the anterior region of the protozoan and in cytoplasmic organelles. Special organization of the membrane particles were also seen in the region of association of the recurrent flagellum to the cell body.

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Publication Dates

  • Publication in this collection
    15 June 2009
  • Date of issue
    Dec 1990
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