Abstract
A rapid and simple technique for the purification of Toxoplasma gondii tachyzoites was developed. Highly purified parasites were obtained from the peritoneal exudates of infected mice by means of two consecutive discontinous sucrose gradients run at low speed (10,000xg, 30 min). Parasites obtained by this method conserved its biological activity. Hybridizations tudies with DNA from healthy mice and from purified tachyzoites preparations demonstrated that Toxoplasma gondii tachyzoites DNA could be obtained with better than 90 per cents purity. Preliminary studies with DNA endonucleases showed the presence in the tachyzoites genome of highly repetitives sequences.
Toxoplasma gondii; tachyzoites purification; DNA repetitive sequences
Toxoplasma gondii: a rapid method for the isolation of pure tachyzoites: preliminary characterization of its genome
Juan Carlos Garberi
Jorge Carlos Blanco1
Sergio Oscar Angel1
Viviana Pzsenny1
Maria Cristina Arakelian1
Ana Pastini1
Centro de Investigaciones Medicas Albert Einstein, Laboratorio de Biologia Molecular, Buenos Aires, Argentina
A rapid and simple technique for the purification of Toxoplasma gondii tachyzoites was developed. Highly purified parasites were obtained from the peritoneal exudates of infected mice by means of two consecutive discontinous sucrose gradients run at low speed (10,000xg, 30 min). Parasites obtained by this method conserved its biological activity. Hybridizations tudies with DNA from healthy mice and from purified tachyzoites preparations demonstrated that Toxoplasma gondii tachyzoites DNA could be obtained with better than 90 per cents purity. Preliminary studies with DNA endonucleases showed the presence in the tachyzoites genome of highly repetitives sequences.
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Publication Dates
-
Publication in this collection
15 June 2009 -
Date of issue
Dec 1990
