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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.86 no.2 Rio de Janeiro Apr./June 1991

http://dx.doi.org/10.1590/S0074-02761991000200006 

Ultrastructural and stereological analysis of trypanosomatidis of the genus Endotrypanum

Maurílio J. Soares1 

Angela H. C. S. Lopes2 

Wanderley de Souza1 

Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Ultraestrutura Celular e Microscopia Eletrônica, Rio de Janeiro, Brasil

Universidade Federal do Rio de Janeiro, Instituto de Microbiologia, Rio de Janeiro, Brasil

ABSTRACT

Culture forms of four strains of Endotrypanum (E. schaudinni and E. monterogeii) were processed for transmission electron microscopy and analyzed at the ultrastructural level. Quantitative data about some cytoplasmic organelles were obeined by stereology. All culture forms were promastigotes. In their cytoplasm four different organelles could be found: lipid inclusions (0,2-0,4 µm in diameter), mebrane-bounded vacuoles (0.10-0,28 µm in diameter), glycosomes (0,2-0,3 µm in diameter), and the mitochondrion. The kenetoplast appears as a thin band, except for the strain IM201, which possesses a broader structure, and possibly is not a member of this genus. Clusters of virus-like particles were seen in the cytoplasm of the strain LV88. The data obtained show that all strains have the typical morphological feature of the trypanosomatids. Only strain IM201 could be differentiated from the others, due to its larger kenetoplast-DNA network and its large mitochondrial and glycosomal relative volume. The morphometrical data did not allow the differentiation between E. schaudinni (strains IM217 and M6226) and E. monterogeii (strain LV88).

Key words: trypanosomatid; Endotrypanum; ultrastructure; stereology

 

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