Abstract
Histoplasma capsulatum var. capsulatum is a dimorphic fungus that, under special conditions, converts from its more common mycelial form to a yeast-like form. Achieving this conversion, however, has been problematical for researchers. The present study tested conversion rates in ten Histoplasma capsulatum var. capsulatum strains using seven culture media, four of wich were conventional and three novel. One of our novel media, MLGema, induced complete conversion, of two strains within five days of incubation at 35 degrees centigrades, and of all strains that eventually converted by the time of the second subculturing transfer, under defined experimental conditions. MLGema is also inexpensive and easy to produce.
Histoplasma capsulatum var. capsulatum; growth media; yeast-like form conversion
A medium for inducing conversion of Histoplasma capsulatum var. capsulatum into its yeast-like form
R. Fressatti1
V. L. Dias-Siqueira1
T. I. E. Svidzinski1
F. Herrero1
C. Kemmelmeier1
Universidade Estadual de Maringá, Departamento de Bioquímica, Departamento de Análises Clínicas, Maringá, Brasil
Histoplasma capsulatum var. capsulatum is a dimorphic fungus that, under special conditions, converts from its more common mycelial form to a yeast-like form. Achieving this conversion, however, has been problematical for researchers. The present study tested conversion rates in ten Histoplasma capsulatum var. capsulatum strains using seven culture media, four of wich were conventional and three novel. One of our novel media, MLGema, induced complete conversion, of two strains within five days of incubation at 35 degrees centigrades, and of all strains that eventually converted by the time of the second subculturing transfer, under defined experimental conditions. MLGema is also inexpensive and easy to produce.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
Mar 1992
