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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.87 no.1 Rio de Janeiro Jan./Mar. 1992 

Some biological properties of the human amniotic membrane interferon

P. C. P. Ferreira1 

L. de la Roque2 

V. M. Rumjanek2 

R. R. Golgher

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Microbiologia. Laboratório de Vírus, Belo Horizonte, Brasil

Instituto Nacional do Câncer, Centro de Pesquisa Básica, Rio de Janeiro, Brasil


Human amniotic interferon was investigated to define the species specificity of its antiviral action and compare its anti-cellular and NK cell stimulating activities with those of other human interferons. The antiviral effect was titrated in bovine (RV-IAL) and monkey (VERO) cells. Amniotic interferon exhibited, in bovine cells, 5% of the activity seen in monkey cells, while α interferon displayed 200%. No effect was detected with either beta or gamma interferon in bovine cells. Daudi cells were exposed to different concentrations of various interferons and the cell numbers were determined. The anticellular effect of the amniotic interferon reached its peak on the third day of incubation. Results suggested a higher activity for α and gamma interferons and a lower activity for beta when compared to amniotic interferon. Using total mononuclear cells as effector cells and K 562 as target cell in a 51Cr release assay, it was demonstrated that low concentrations of amniotic interferon consistently stimulated NK cell activity in cells derived from several donors, the results indicating a higher level of activity with this interferon than with α and beta interferons.


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