Abstract
Characterization is given of a new parasite, Leishmania equatoriensis sp.n. wich was isolated from the viscera of a sloth (Choloepus hoffmanni) and a squirrel (Sciurus granatensis), captured in humid tropical forest onthe Pacific Coast of Ecuador. Data based on biological and molecular criteria, as well as numerical zymotaxonomical analysis, indicate that this parasite is a new species of the L. brasiliensis complex. L. equatoriensis is cleary distinguishable form all other known species within this complex, using the following molecular criteria: reactivity patterns with specific monoclonal antibodies, isoenzyme electrophoresis, and restriction-endonuclease fragment patterns of kinetoplast DNA (k-DNA).
Leishmania equatorensis sp. n.; Protozoa; Kinetoplastida; Trypanosomatidae; mammalian reservoirs; molecular characterization; monoclonal antibodies; isoenzyme electrophoresis; kinetoplast DNA analysis
Description of Leishmania equatorensis sp. n. (Kinetoplastida: Trypanosomatidae), a new parasite infecting arboreal mammals in Ecuador
Gabriel Grimaldi Júnior1
Richard D. Kreutzer2
Yoshihisa Hashiguchi3
Eduardo A. Gomez4
Tatsuyuki Mimory5
Robert B. Tesh6
Instituto Oswaldo Cruz, Departamento de Imunologia, Rio de Janeiro, Brasil
Youngstown State University, Department of Biology, Youngstown, USA
Kochi Medical School, Department of Parasitology, Nankoku, Japan
Instituto Nacional de Higiene y Medicina tropical, Departamento de Parasitologia, Guayaquil, Ecuador
Kumamoto University School of Medicine, Department of Parasitic Diseases, Kumamoto, Japan
Yale University School of Medicine, Department of Epidemiology and Public Health, New Haven, USA
Characterization is given of a new parasite, Leishmania equatoriensis sp.n. wich was isolated from the viscera of a sloth (Choloepus hoffmanni) and a squirrel (Sciurus granatensis), captured in humid tropical forest onthe Pacific Coast of Ecuador. Data based on biological and molecular criteria, as well as numerical zymotaxonomical analysis, indicate that this parasite is a new species of the L. brasiliensis complex. L. equatoriensis is cleary distinguishable form all other known species within this complex, using the following molecular criteria: reactivity patterns with specific monoclonal antibodies, isoenzyme electrophoresis, and restriction-endonuclease fragment patterns of kinetoplast DNA (k-DNA).
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
June 1992
