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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.87 no.2 Rio de Janeiro Apr./June. 1992

http://dx.doi.org/10.1590/S0074-02761992000200013 

Molecular and biological diversity of HIV-1 in Brazil

José Carlos Couto-Fernandez1 

Mariza Gonçalves Morgado2 

Jairo Ivo dos Santos3 

Bernardo Galvão-Castro

Instituto Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz, Laboratório Avançado de Saúde Pública, Salvador, Brasil

Instituto Oswaldo Cruz, Departamento de Imunologia, Rio de Janeiro, Brasil

Instituto Oswaldo Cruz, Centro de Pesquisas Gonçalo Moniz, Laboratório Avançado de Saúde Pública, Rio de Janeiro, Brasil

ABSTRACT

To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.

Key words: Brazilian HIV; virulence; restriction analysis; viral diversity

 

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