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vol.87 suppl.3Human IgG responses against the N-terminal region of Merozoite Surface Protein 1 of Plasmodium vivaxCharacterization of a Plasmodium falciparum mutant that has deleted the majority of the gametocyte-specific Pf11-1 locus author indexsubject indexarticles search
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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276
On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.87  suppl.3 Rio de Janeiro  1992 

A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence

D. J. Kemp1  , J. Thompson1  , D. A. Barnes1  , T. Triglia1  , F. Karamalis1  , C. Petersen2  , G. V. Brown1  , K. P. Day3 

The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia

University of California, San Francisco, USA

Imperial College of Science and Technology, London, England


Many lines of Plasmodium falciparum undrgo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.

Key words: chromosome deletion; cytoadherence; Plasmodium falciparum; pathology; PfEMP1


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