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Extrachromosomal nucleic acids in bovine babesia

Abstract

Two kinds of small extrachromosomal nucleic acid elements were found in the bovine babesias, Babesia bovis and B. bigemina. One element with an apparent size of 5.5 kilobase pairs (kbp) is a double stranded RNA related to virus like particles. Another molecule is a double stranded DNA with a molecular size of about 6.2 kbp. Southern blot comparison of restriction DNA fragments of the latter molecule, which is present in both B. bovis and B. bigemina is described.

Babesia bovis; Babesia bigemina; mitochondrial DNA; DNA element


ABSTRACT

Extrachromosomal nucleic acids in bovine babesia

Isidro Hotzel1

Rhona Carol Johnston2

Julia Taque2

Nara Amélia Rosa Farias3

Joao Carlos Gonzalés1

Luiz Shozo Ozaki3

Universidade Federal do Rio Grande do Sul, Faculdade de Veterinária, Porto Alegre, Brasil

Universidade Federal do Rio Grande do Sul, Departamento de Bioquímica, Porto Alegre, Brasil

Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Brasil

Two kinds of small extrachromosomal nucleic acid elements were found in the bovine babesias, Babesia bovis and B. bigemina. One element with an apparent size of 5.5 kilobase pairs (kbp) is a double stranded RNA related to virus like particles. Another molecule is a double stranded DNA with a molecular size of about 6.2 kbp. Southern blot comparison of restriction DNA fragments of the latter molecule, which is present in both B. bovis and B. bigemina is described.

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Publication Dates

  • Publication in this collection
    04 June 2009
  • Date of issue
    1992
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