Abstract
The P126 protein, a parasitosphorus vacuole antigen of Plasmodium falciparum has beenshoen to induce protective immunity in Saimiri and Aotus monkeys. In the present work we investigated its immunogenicity. Our results suggest that the N-term of P126 is poorly immunogenic and antibody response against the P126 could be under a MHC restricted control in C57BL/6(H-2b) mice, which could be problematic in ternms of a use of the P126 in a vaccine program. However, we observed that a synthetic peptide, copying the 6 octapeptide repeat corresponding to the N-term of the P126, induces an antibody response to the native molecule in C57BL/6 non-responder mice. Moreover, the vaccine-P126 recombinant induced anmtibodies against the N-term of the molecule in rabbits while the unprocessed P126 did not.
Plasmodium falciparum; P126; synthetic peptide; vaccine-P126 recombinant; immunogenicity; immunization
Immunogenicity and antigenicity of the N-term repeat amino acid sequence of the Plasmodium falciparum P126 antigen
Dalma Maria Banic1
Marc Bossus2
Patrick Delplace1
André Tartar3
Héléne Gras-Masse2
Valérie Conseil1
Christine Mazingue4
Charles Taisne5
Daniel Camus
B.P. 39, INSERM U-42, Villeneuve d'Aseq, France
Institut Pasteur, CNRS 1309, Chimie des Biomolécules, Lille, France
Institut Pasteur, CNRS 1309, Chimie des Biomolécules, Lille, Frances.af
Institut Pasteur, CNRS 624, INSERM U 167, Lille, France
TROY, VIROGENETICS, New York, USA
The P126 protein, a parasitosphorus vacuole antigen of Plasmodium falciparum has beenshoen to induce protective immunity in Saimiri and Aotus monkeys. In the present work we investigated its immunogenicity. Our results suggest that the N-term of P126 is poorly immunogenic and antibody response against the P126 could be under a MHC restricted control in C57BL/6(H-2b) mice, which could be problematic in ternms of a use of the P126 in a vaccine program. However, we observed that a synthetic peptide, copying the 6 octapeptide repeat corresponding to the N-term of the P126, induces an antibody response to the native molecule in C57BL/6 non-responder mice. Moreover, the vaccine-P126 recombinant induced anmtibodies against the N-term of the molecule in rabbits while the unprocessed P126 did not.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
1992
