Abstract
Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were administered. This led to the development of specific antibodies detected by IFAT, ELISA or immunobloting which inhibited merozoite reinvasion in in vitro P. falciparum cultures. This activity seems to be associated with rhoptry proteins contained in fractions Pf F2 and Pf F4.
Plasmodium falciparum; protein fractions; in vitro parasite inhibition; rhoptry proteins
Study of humoral immune response in mammals immunized with Plasmodium falciparum antigenic preparations
Antonio B. Cruz Cubas1
Flavia Cunha2
Isére Kuiatse Nitcheu
Laurence Rolland
Liliane Ciceron
Alexandre B. Carvalho2
André F. Furtado2
Loic Monjour
Faculté de Médicine Pitié-Salpêtrière, INSERM U 313, Paris, France
FIOCRUZ, C. P. Aggeu Magalhães, Recife, Brasil
Six Plasmodium falciparum protein fractions, isolated under reducing conditions, were used to immunize mice, rabbits and the squirrel monkey Saimiri sciureus. Five or seven subcutaneous injections of each antigenic preparation, in conjunction with Freund's complete or incomplete adjuvants, were administered. This led to the development of specific antibodies detected by IFAT, ELISA or immunobloting which inhibited merozoite reinvasion in in vitro P. falciparum cultures. This activity seems to be associated with rhoptry proteins contained in fractions Pf F2 and Pf F4.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
1992
