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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.87  suppl.4 Rio de Janeiro  1992

http://dx.doi.org/10.1590/S0074-02761992000800016 

Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement

F. Juarez Ramalho-Pinto1 

Ermelinda M. R. D. Carvalho1 

Maria de Fátima M. Horta1 

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica-Imunologia. Laboratório de Imunologia Molecular das Doenças Parasitárias, Belo Horizonte, Brasil

ABSTRACT

Schistosomula of Schistosoma mansoni became resistant to antibody-dependent complement damage in vitro after pre-incubation with normal human erythrocytes (NHuE) whatever the ABO or Rh blood group. Resistant parasites were shown to acquire host decay accelerating factor (DAF) , a 70 kDa glycoprotein attached to the membrane of NHue by a GPI anchor. IgG2a mAb anti-human DAF (IA10) immunoprecipitated a 70 kDa molecule from 125I-labeled schistosomula pre-incubated with NHuE and inhibited their resistance to complement-dependent killing in vtro. Incubationof schistosomula with erytrocytes from patients with paroxsimal nocturnal hemoglobinuria (PNHE) or SRBC, wich are DAF-deficient, did not protect the parasites from complement lesion. Supernatant of 100,000 x g collected from NHuE incubated for 24 h in defined medium was shown to contain a soluble form of DAF and to protect schistosomula from complement killing. Schistosomula treated with trypsin before incubation with NHuE ghosts did not become resistant to complement damage. On the other hand, pre-treatment with chymotrypsin did not interfere with the acquisition of resistance by the schistosomula. These results indicate that, in vitro, NHuE DAF can be transferred to schistosomula in a soluble form and that the binding of this molecule to the parasite surface is dependent upon trypsin-sensitive chymotrypsin-insensitive polipeptide(s) present on the surface of the worm.

Key words: Schistosoma mansoni schistosomula; complement; human decay accelerating factor

 

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