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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.88 no.3 Rio de Janeiro July/Sept. 1993

http://dx.doi.org/10.1590/S0074-02761993000300008 

Description of Leishmania (Leishmania) forattinii sp. n., a new parasite infecting opossums and rodents in Brazil

Elizaide L. A. Yoshida1 

Cesar A Cuba Cuba2 

Raquel da Silva Pacheco3 

Elisa Cupolillo3 

Celso Cruz Tavares4 

Gerzia M. C. Machado5 

Hooman Momen3 

Gabriel Grimaldi Junior5 

Universidade Estadual Paulista, IBBMA, Departamento de Parasitologia, Botucatu, Brasil

Unviersidade de Brasília, FCS, Departamento de Patologia, Brasília, Brasil

Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil

Universidade Estadual do Rio de Janeiro, IB, Departamento de Biologia Celular e Genética, Rio de Janeiro, Brasil

Instituto Oswaldo Cruz, Departamento de Imunologia, Rio de Janeiro, Brasil

ABSTRACT

A new parasite species of Leishmania is described, L. (Leishmania) forattinii sp. n., which was isolated from a pooled triturate of liver and spleen of a opossum (Didelphis marsupialis aurita) and from skin samples from a rodent (Proechmys iheringi denigratus), captured in primary forest on the Atlantic Cost of Brazil. Our results on the basis of biological and molecular criteria indicate that this taxonomically distinct parasite ias a new species of the L. mexicana complex, but closely related to L. (L.) aristidesi Laison & shaw, 1979, as revelated by phenetic and phylogenetic numerical analyses of the enzyme data. L. forattinii was clearly distinguishable from other Leishmania species of the genus usisng enzyme electrophoresis, monoclonal antibodies, molecular karyotypes, analysis of restriction enzyme digestion patterns of kinetoplast DNA (kDNA), as well as the use of kDNA hybridization procedures.

Key words: Leishmania (L.) forattinii sp. n.; Protozoa; Kinetoplastida; Trypanosomatidae; mammalian resevoirs; molecular characterization; monoclonal antibodies; isoenzyme electrophoresis; kenetoplast DNA analysis; molecular karyotype analysis

 

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