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On-line version ISSN 1678-8060
Mem. Inst. Oswaldo Cruz vol.89 no.2 Rio de Janeiro Apr./Jun. 1994
The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami, Recife, Brasil
Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, Brasil
Universidade Federal de Pernambuco, Departamento de Medicina Tropical, Recife, Brasil
Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.