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On-line version ISSN 1678-8060
Mem. Inst. Oswaldo Cruz vol.89 no.2 Rio de Janeiro Apr./Jun. 1994
The use of ferromagnetic dacron as solid-phase in enzyme immunoassaysLeãoAna Maria dos A. CarneiroCarvalho JuniorLuiz B.MalagueñoElizabeth Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami Recife Brasil Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal Recife Brasil Universidade Federal de Pernambuco, Departamento de Medicina Tropical Recife Brasil 061994892189193Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.magnetic ELISAferromagnetic dacronhuman serum albuminFull text available only in PDF format.Texto completo disponível apenas em PDF.