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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.89 no.2 Rio de Janeiro Apr./Jun. 1994 

The use of ferromagnetic dacron as solid-phase in enzyme immunoassays

Ana Maria dos A. Carneiro Leão1 

Luiz B. Carvalho Junior2 

Elizabeth Malagueño3 

Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami, Recife, Brasil

Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, Brasil

Universidade Federal de Pernambuco, Departamento de Medicina Tropical, Recife, Brasil


Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.

Key words: magnetic ELISA; ferromagnetic dacron; human serum albumin


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