Abstract
Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
magnetic ELISA; ferromagnetic dacron; human serum albumin
The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
Ana Maria dos A. Carneiro Leão1
Luiz B. Carvalho Junior2
Elizabeth Malagueño3
Universidade Federal de Pernambuco, Laboratório de Imunopatologia Keizo Asami, Recife, Brasil
Universidade Federal Rural de Pernambuco, Departamento de Morfologia e Fisiologia Animal, Recife, Brasil
Universidade Federal de Pernambuco, Departamento de Medicina Tropical, Recife, Brasil
Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
Full text available only in PDF format.
Texto completo disponível apenas em PDF.
Publication Dates
-
Publication in this collection
01 June 2009 -
Date of issue
June 1994
