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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Mem. Inst. Oswaldo Cruz vol.89 no.3 Rio de Janeiro Jul./Sept. 1994

http://dx.doi.org/10.1590/S0074-02761994000300018 

Ultrastructural study on experimental infection of rotavirus in a murine heterologous modelMajerowiczSelmaKubelkaClaire FernandesStephensPauloBarthOrtrud Monika Instituto Oswaldo Cruz, Departamento de Virologia Rio de Janeiro Brasil 091994893395402Viral replication, histopathological and ultrastructural changes were observed for a period of nine days in the small intestine of suckling mice infected with a simian rotavirus (SA11). Samples taken from duodenum, jejunun and ileum were prepared for light microscopy, transmission and scanning electron microscopy analysis. Histopathologic effect could be detected within 8 hr post-infection, when only a few altered cells were observed. Damage was extensive after 16 hr post-infection, showing swollen enterocytes and reduced and irregularly oriented microvilli at intestinal villi tips. Virus particles were detected at 16 and 48 hr post-infection, budding from the viroplasm into the rough endoplasmic reticulum cisternae in ileum enterocytes. Clear evidence of viral replication, observed by electron microscopy was not described before in heterologous murine models. Regeneration of the intestinal villi began at the third day post-infection. Despite some differences observed in clinical symptoms and microscopic analysis of homologous and heterologous rotavirus infections, we concluded that mechanisms of heterologous rotavirus infection in mice follow similar patterns to those observed in the homologous models.simian rotavirusultrastructureheterologous modelFull text available only in PDF format.Texto completo disponível apenas em PDF.