Abstract
We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
Schistosoma mansoni; automated DNA sequencing; EST; gene identification
Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library
Glória R. Franco1
Andrew J. G. Simpson2
Sérgio D. J. Pena1
UFMG, Instituto de Ciências Biológicas, Departamento de Bioquímica, Belo Horizonte, Brasil
FIOCRUZ, Centro de Pesquisas René Rachou, Laboratório de Biologia Molecular, Belo Horizonte, Brasil
We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequencers. ESTs can be used to identify genese onf the basis of their homology whith sequences from other species deposited in DNA or protein databases. Trasncripts with sequences without matches in teh databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
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Publication Dates
-
Publication in this collection
01 June 2009 -
Date of issue
Apr 1995
