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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.91 no.2 Rio de Janeiro Mar./Apr. 1996 



Evaluation of Enzyme-Linked Immunosorbent Assay for the serodiagnosis of tuberculosis in patients with AIDS in Cuba



Ernesto Montoro; Raúl Díaz; Miguel Echemendía; José A Valdivia

Instituto de Medicina Tropical "Pedro Kourí" (IPK), Laboratorio Nacional de Referencia de Mycobacteria y Tuberculosis, Apdo 601, Marianao 13, Ciudad de La Habana, Cuba



Key words: ELISA - tuberculosis - AIDS - PPD - Cuba



Tuberculosis is a major complication of AIDS, and the diagnosis of tuberculosis in patients with AIDS is difficult because the presentation of the former disease is often not typical and because the yield of standard microbiological techniques may be low (TM Daniel et al. 1994 Tubercle Lung Dis 75: 33-37).

Recently, we used an Enzyme-Linked Immunosorbent Assay (ELISA) for detection of IgG antibodies to purified protein derivative (PPD) antigen of Mycobacterium tuberculosis for the diagnosis of pulmonary tuberculosis. The specificity and the sensitivity were 95.33% and 82% respectively. We used a cut off point of 0,320 (E Montoro et al. 1994 Rev Cub Med Trop 46: 90-93).

In this paper we applied the ELISA from sera (25) of patients of tuberculosis with AIDS in Cuba during an outbreak from July 1993 to March 1994. Sera (25) from patients with tuberculosis without AIDS were also included. In all cases, diagnosis was bacteriologically confirmed at the same time.

Positive results in the ELISA test were obtained in 5 out of 25 sera (20%) from patients with AIDS-tuberculosis, however in 21 sera (84%) from patients with tuberculosis without AIDS were positive. As it is noted the sensitivity of the test in AIDS-tuberculosis patients was much lower than in our previously described study (Montoro et al. loc. cit.).

Our results agree with other reports using ELISA for detection of PPD as antigen and showing severely diminished sensitivity in AIDS patients (IN Kantor et al. 1990 Am Rev Respir Dis 141: A264, L Barrera et al. 1992 Tubercle Lung Dis 73: 187-191). This could be explained by the profound changes in cell immunity and the impairment of humoral immunity in AIDS patients (JFC Figueiredo, AA Machado 1992 Braz J Med Biol Res 25: 611-618).

In Uganda, a country with high rates of AIDS and tuberculosis infections, Daniel et al. (loc. cit.) studied an ELISA for IgG antibody to a 30,000 dalton antigen of M. tuberculosis. Test sensitivity dropped from 62% in non HIV infected tuberculosis patients to 28% in HIV infected patients. However, although the prevalence of both infections, is low in Cuba, and in spite of the small number of sera studied here our results coincide with Daniel regarding this technique in AIDS patients with tuberculosis.

In summary, our findings show that the sensitivity of ELISA technique may decrease when applying it in AIDS patients, independent of the rate of prevalence of both diseases.



Received 3 March 1995
Accepted 9 November 1995



This work received financial support from SAREC (Sweden).

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