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Print version ISSN 0074-0276
Mem. Inst. Oswaldo Cruz vol.107 no.3 Rio de Janeiro May 2012
Renata Carvalho de OliveiraI, +; Alexandro GuterresI; Carlos Guerra SchragoII; Jorlan FernandesI; Bernardo Rodrigues TeixeiraIII; Suzana ZeccerIV; Cibele R BonvicinoIII, V; Paulo Sérgio D'AndreaIII; Elba Regina Sampaio de LemosI
ILaboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil
IIDepartamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
IIILaboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil
IVSecretaria Estadual de Saúde de Santa Catarina, Florianópolis, SC, Brasil 5Instituto Nacional do Câncer, Rio de Janeiro, RJ, Brasil
We characterised hantaviruses circulating in different Akodon rodent species collected in midwestern Santa Catarina (SC), southern Brazil, where the Jabora hantavirus (JABV) strain was first identified in Akodon montensis. Genetic and phylogenetic analyses based on a partial S segment indicated that, in SC, Akodon paranaensis and A. montensis carried the same type of hantavirus. Additionally, we conducted the first genomic characterisation of the complete S segment from the Brazilian JABV strain. This is the first report of A. paranaensis infected with the JABV.
Key words: phylogenetic analyses - rodents - hantavirus - Santa Catarina
Hantavirus pulmonary syndrome (HPS) is caused by the emerging rodent-borne viruses of the genus Hantavirus (Nichol et al. 1993). Since 1993, over 1,400 cases have been identified in 14 states in Brazil. Santa Catarina (SC) (southern Brazil, 27º10'S 51º44'W) is the second most affected Brazilian state, with a large number of cases (n = 226) (Brazilian Health Ministry Report on Hantavirus cases 1993-2011, unpublished data). Currently, there are five hantaviruses associated with HPS in Brazil: Juquitiba/Araucaria, Araraquara, Laguna Negra, Anajatuba and Castelo dos Sonhos; these are carried by Oligoryzomys nigripes, Necromys lasiurus, Calomys sp., Oligoryzomys fornesi and Oligoryzomys utiaritensis, respectively (Johnson et al. 1999, Suzuki et al. 2004, Raboni et al. 2005a, 2009a, Rosa et al. 2010, Travassos da Rosa et al. 2011). Two other hantaviruses, Rio Mearim and Jabora (JABV) have been identified in the rodent species Holochilus sciureus and Akodon montensis, respectively, but their roles in human disease have not been determined (Rosa et al. 2005, Oliveira et al. 2011).
Hantaviruses can co-circulate in the same locality and can be maintained side-by-side in different rodent species, as reported for Juquitiba and JABV related viruses and other Old World hantaviruses (Artois et al. 2007, Chu et al. 2009, Raboni et al. 2009b, Razzauti et al. 2009, Oliveira et al. 2011). In this study, we have identified JABV, which is associated with A. montensis and Akodon paranaensis, two related and sympatric rodent species in Midwestern SC (Jabora, 27º09'S 51º47'W). These two species of Akodon are widely distributed in the central Southern Cone of South America and may be locally abundant in their preferred habitat (Musser & Carleton 2005). Additionally, we conducted the first genomic characterisation of the complete S segment from the JABV strain to better characterise the hantaviruses circulating in these two related rodent species.
RNA was extracted from lung tissue samples of five antibody-positive rodents from A. paranaensis (Akp8032, Akp8048) and A. montensis (Akm6266, Akm6943 and Akm9635) species, according to the manufacturer's instructions from the Trizol® Plus RNA Purification Kit. The rodent samples were analysed using the polymerase chain reaction with reverse transcription and nested reactions. For direct sequencing of overlapping amplimers, generic primer combinations (n = 8) were used for amplification and sequencing of the complete genomic S segment, including published (Raboni et al. 2005b, Oliveira et al. 2011) and unpublished (S Levis 2005, unpublished observations, A Guterres 2011, unpublished observations) oligonucleotide sequences. These sequences were designed based on the conserved regions of the S segment among South American hantaviruses (primers available on request). Amplicons of the expected size (approximately 1,800 bp) were recovered from two samples: one from A. montensis (Akm9635) and another from A. paranaensis (Akp8048). Sequence alignments were run in SeaView, using the MUSCLE algorithm. Obtained virus sequences (partial and complete) are accessible from GenBank (JN232078_Akm9635, JN232080_Akp8048 and JN232081_Akp8032). Phylogenetic relationships were estimated using (i) a maximum likelihood (ML) phylogenetic inference method with 1,000 bootstrap replicates, implemented in PhyML 3 (Guindon & Gascuel 2003) under the GTR+G model of sequence evolution, which was chosen after hierarchically testing alternative models by computing likelihood ratios and (ii) a Bayesian Markov chain Monte Carlo method implemented in MrBayes v3.1.2 (Ronquist & Huelsenbeck 2003), using the GTR+G model of nucleotide substitution. Two simultaneous runs of four chains each were run for 1 million generations and sample frequency = every 100th generation; a consensus tree (burn-in of 25%) was constructed from the remaining trees. Posterior probabilities above 0.95 and bootstrap values above 70% at the nodes were accepted as significant. We also assessed the phylogenetic relationships between JABV and other hantaviruses with a partial section of the S segment sequence (700 nucleotides due to the large number of partial sequences available).
Phylogenetic trees calculated by ML (not shown) and Bayesian methods, based on partial and complete sequences, indicated similar topology at the relevant nodes (Fig. 1). Phylogenetic analyses of these sequences (partial and complete) indicated that all hantaviruses carried by A. montensis and A. paranaensis form a distinct and monophyletic lineage. ML and Bayesian analyses based on partial S segment indicated that the sequences circulating in A. paranaensis are closely related to A. montensis viruses from Brazil, Argentine and Paraguay (JABV/AC210py/AAIV). Although A. montensis sequences are not monophyletic, A. paranaensis (Akp8032 and Akp8048) sequences are grouped with significant support and are tightly associated with the A. montensis virus strain (Fig. 1). The JABV-like virus clade could be divided into two well-supported subclades: one composed of Paraguayan and Argentinean viruses and the other composed of Brazilian viruses (Akp and Akm samples). The ML tree that was constructed using the amino acid sequences of the S segment indicated highly similar bootstrap values and a branching pattern obtained from the nucleotide sequence phylogenetic analysis (not shown).
In pairwise comparisons of a nucleotide sequence, calculated using MEGA 5 (Tamura et al. 2011), the genetic distance among JABV strains from Brazilian A. montensis and A. paranaensis ranged from 0.1-3.4% and amino acid derived differences ranged from 0-1.3%. This suggests that spillover infection of JABV-related viruses is actively occurring among Akondontini rodent species in southern Brazil. Furthermore, the nucleotide differences between JABV strains from Brazil and JABV/AAIV-related viruses from Paraguay and Argentina (Ac210py) ranged from 11.5-14.7% (Table).
All five Akodon specimens were karyotyped to confirm morphologic identification. Phylogenetic reconstructions of three rodent specimens (Akm6943, Akp8048, Akp8032), based on the mitochondrial DNA cytochrome b gene (Smith & Patton 1993), were also obtained. These reconstructions were used to confirm species identification and to estimate phylogenetic relationship of the hantavirus-positive specimens, using the same phylogeny model of evolution and parameters described above for hantaviruses. All rodent specimens collected were fixed in 10% formalin or prepared as skin and skull and placed as voucher specimens in the mammal collection of the National Museum of the Federal University of Rio de Janeiro.
Karyologic analyses confirmed that three Akodon specimens belong to A. montensis (2n = 24, FNa = 42) and the other two specimens to A. paranaensis (2n = 44, FNa = 44). The Bayesian and ML (not shown) trees indicated similar topologies for Akodontini (Fig. 2). These analyses grouped the haplotypes of Akodon sequenced specimens (Akm6943, Akp8048, Akp8032) with the GenBank sequence of A. montensis and A. paranaensis.
Genetic and phylogenetic analyses based on S partial and complete segments indicated that, in SC, A. paranaensis and A. montensis carried the same type of hantavirus. According to some ecological mathematics models, the presence of multiple hosts increases the possibility of disease emergence (McCormack & Allen 2007). In this study, JABV was identified for the first time in A. paranaensis. The phylogenies obtained from S segments indicate that the A. paranaensis strain is monophyletic and related to the virus circulating in the sympatric A. montensis. Spillover of JAB-like virus from its real host to other sympatric rodent species cannot be excluded and, therefore, further investigation of this issue is needed. Studies utilising phylogenetic methods to generate and compare evolutionary scenarios of hantaviruses and their rodent hosts are critical to better understanding the evolution of hantaviruses, especially in South America. Additionally, a longitudinal study and new rodent collection expeditions in different areas are needed to elucidate whether A. paranaensis rodents are true reservoirs or only sporadic hosts.
To Antônio Caldas, Epidemiologic Surveillance and Manager of the Zoonotic Control of the Santa Catarina State Health Department, for their dedication during the development of the studies in Jabora, and to Dalir Alberto Ruaro, secretary of Jabora Health Department, for their logistic and continued support.
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Received 8 July 2011
Accepted 24 November 2011
Financial support: CNPq/FIOCRUZ (403050/2004-9)