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Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276versão On-line ISSN 1678-8060

Mem. Inst. Oswaldo Cruz vol.110 no.4 Rio de Janeiro jun. 2015  Epub 29-Maio-2015 

Genome Announcement and Highlights

Draft genome sequences of three NDM-1-producing Enterobacteriaceae species isolated from Brazil

Polyana Silva Pereira 1  

Rodolpho Mattos Albano 2  

Marise Dutra Asensi 1  

Ana Paula D’Alincourt Carvalho-Assef 1   +  

1Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil

2Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil


The emergence of multidrug-resistant Enterobacteriaceae strains producing carbapenemases, such as NDM-1, has become a major public health issue due to a high dissemination capacity and limited treatment options. Here we describe the draft genome of three NDM-1-producing isolates: Providencia rettgeri(CCBH11880), Enterobacter hormaecheisubsp. oharae (CCBH10892) and Klebsiella pneumoniae (CCBH13327), isolated in Brazil. BesidesblaNDM-1, resistance genes to aminoglycosides [aadA1, aadA2,aac(6’)-Ib-cr] and quinolones (qnrA1,qnrB4) were observed which contributed to the multidrug resistance profile. The element ISAba125 was found associated to theblaNDM-1 gene in all strains.

Key words: NDM-1; Brazil; Enterobacteriaceae

The emergence of carbapenemase-producing Enterobacteriaceae has become a major public health issue worldwide due to a high dissemination capacity and limited treatment options (Nordaman et al. 2011). NDM is a metallo-beta-lactamase first reported in 2009 (Yong et al. 2009) and, now, it has already been detected in several countries worldwide. In Brazil, this carbapenemase was first described in a Providencia rettgeri isolate from the city of Porto Alegre, state of Rio Grande do Sul (RS) (South Region of Brazil), in 2013 (Carvalho-Assef et al. 2013). Then, the detection of six clonally related NDM-producingEnterobacter hormaechei subsp. oharae isolates was reported from the same public hospital (Carvalho-Assef et al. 2014). After that, nine NDM-1-producing Enterobacter cloacae complex isolates and two Morganella morgannii were also recovered from three different hospitals in Porto Alegre (Rozales et al. 2014). Recently, NDM-1-producing bacteria have been described in the state of Rio de Janeiro (RJ) (Southeast Region of Brazil) (Pereira et al. 2014). In 2014, a NDM-1-producingAcinetobacter baumannii was also detected in Londrina, state of Paraná (South Region of Brazil) (Pillonetto et al. 2014).

Here, we aim to announce the draft genome of three NDM-1-producing isolates: P. rettgeri (CCBH11880), isolated from a surgical wound of a patient from RS,E. hormaechei subsp. oharae (CCBH10892), isolated from a rectal swab of another patient in RS, and Klebsiella pneumoniae(CCBH13327), isolated from a rectal swab of a patient in the city of Rio de Janeiro, RJ.

Initially all of the strains were tested against different antimicrobial drugs and showed multidrug resistance profiles. Genomic DNA of all strains was extracted using QIAamp DNA Blood Mini Kit (Qiagen, Germany). Whole genome shotgun libraries from each strain were prepared with the Nextera XT DNA Sample Prep kit (Illumina Inc, USA), according to the manufacturer’s instructions, and sequenced on an Illumina MiSeq system with the MiSeq Reagent v.2 500 cycles kit. Sequence reads were then trimmed and filtered using a Phred score >20. The software Geneious v.6.1.7 (Biomatters Ltd, New Zealand) was used to perform de novo assembling. Rapid Annotation using System Technology v.2.0 server was used for genome annotation. Acquired resistance genes were analysed using the ResFinder platform ( The detailed features of all isolates can be found on Table.

TABLE Genetic information about three NDM-1-producing isolates from Brazil 

Isolate feature Providencia rettgerii (CCBH11880) Enterobacter hormaechei (CCBH10892) Klebsiella pneumoniae (CCBH13327)
NCBI acession JSEQ00000000 JSBO00000000 JSER00000000
BioProject PRJNA264579 PRJNA264581 PRJNA264954
Isolation source Surgical wound Rectal swab Rectal swab
City/state of origin Porto Alegre/RS Porto Alegre/RS Rio de Janeiro/RJ
GC content (%) 41 54.5 56.6
Paired end reads (n) 656,560 2,283,589 1,748,579
Genome coverage 32X 106X 72X
Estimated genome size (bp) 4,999,177 5,373,562 6,023,847
Contigs (n) 80 58 106
N50 282.487 277.989 133.213
Coding sequences (n) 4,670 5,134 5,722
RNAs (n) 89 102 99
tRNA (n) 79 89 86
rRNA (n) 10 13 13
Resistance genes aadA1, strA,strB, aadB,aac(6’)-Ib, qnrD, bla OXA-10,bla NDM-1, ere(A),msr(E), mph(E), floR,catA1, sul1,sul2, tet(A),dfrA1, dfrA8 aadA2, aph(3’)-Ia,strA, strB, bla DHA-1,bla ACT-7,bla CTX-M-15,bla NDM-1, qnrB4,ere(A), sul1,tet(A), tet(D) aadA2, aac(3)-IIa,bla SHV-99,bla CTX-M-2,bla CARB-2,bla NDM-1,oqxA, oqxB,qnrA1, catA1,sul1, tet(D), dfrA8

bp: base pair; NCBI: National Center for Biotechnology Information; RJ: state of Rio de Janeiro; RS: state of Rio Grande do Sul.

For the P. rettgeri isolate (CCBH11880) we obtained 656,560 paired end reads of 250 base pairs (bp), which were assembled into 80 contigs. The G+C content for this strain was 41%, considered common for this species. The estimated genome size, comprising all contigs, was 4,999,177 bp. Overall, 4,670 protein coding sequences were found and 89 RNAs were annotated (79 tRNA and 10 rRNA). Acquired resistance genes were searched using the ResFinder platform and different resistance genes were observed such as: aadA1 (GenBank JSEQ01000006.1; 199,767-200,555 bp), strA(GenBank JSEQ01000017.1; 91,398-92,201 bp), strB (GenBank JSEQ01000017.1; 92,201-93,037 bp), aadB (GenBank JSEQ01000041.1; 326-859 bp), aac(6’)-Ib (GenBank JSEQ01000017.1; 2,882-3,400 bp), qnrD (GenBank JSEQ01000028.1; 1,671-2,315 bp),bla OXA-10 (GenBank JSEQ01000017.1; 2,014-2,814 bp),bla NDM-1 (GenBank JSEQ01000024.1; 412-1,224 bp), ere(A)(GenBank JSEQ01000025.1; 295-1,521 bp), msr(E) (GenBank JSEQ01000023.1; 37,090-38,565 bp), mph(E) (GenBank JSEQ01000023.1; 36,150-37,034 bp), floR (GenBank JSEQ01000017.1; 96,173-97,386 bp), catA1 (GenBank JSEQ01000031.1; 870-1,529 bp), sul1(GenBank JSEQ01000017.1; 3,823-4,749 bp), sul2 (GenBank JSEQ01000017.1; 90,522-91,337 bp), tet(A) (GenBank JSEQ01000017.1; 94,373-95,544 bp), dfrA1 (GenBank JSEQ01000006.1; 198,617-199,090 bp) and dfrA8 (GenBank JSEQ01000033.1; 334-843 bp).

Genome sequencing of E. hormaechei subsp. oharae isolate (CCBH10892) generated 2,283,589 paired end reads of 250 bp, yielding 58 contigs after assembly (Geneious v.6.1.7) for a genome size estimative of 5,373,562 bp. A total of 5,134 protein coding sequences and 102 RNAs were observed, being 89 tRNA and 13 rRNA. ResFinder analysis provided the following acquired resistance genes: aadA2 (GenBank JSBO01000022.1; 2,498-3,289 bp), aph(3’)-Ia (GenBank JSBO01000057.1; 354-932 bp), strA (GenBank JSBO01000042.1; 2,178-2,981 bp),strB (GenBank JSBO01000042.1; 2,981-3,817 bp),bla DHA-1 (GenBank JSBO01000036.1; 13,727-14,866 bp),bla ACT-7 (GenBank JSBO01000001.1; 594,635-595,780 bp),bla CTX-M-15 (GenBank JSBO01000007.1; 210,876-211,751 bp), bla NDM-1 (GenBank JSBO01000041.1; 4,501-5,313 bp),qnrB4 (GenBank JSBO01000036.1; 8,959-9,606 bp), ere(A)(GenBank JSBO01000002.1; 405,895-406,937 bp), sul1 (GenBank JSBO01000022.1; 3,707-4,633 bp), tet(A) (GenBank JSBO01000046.1; 2,098-3,297 bp) and tet(D) (GenBank JSBO01000044.1; 1,151-2,335 bp).

The K. pneumoniae isolate (CCB13327) had an estimated genome size of 6,023,847 bp. An assembly (Geneious v.6.1.7) with 106 contigs was achieved with 1,748,579 paired end reads of 250 bp. A total of 5,722 protein coding sequences were observed, including 99 RNA sequences (86 tRNA and 13 rRNA). Acquired resistant genes found were: aadA2 (GenBank JSER01000014.1; 552-1,343 bp),aac(3)-IIa (GenBank JSER01000058.1; 14,738-15,598 bp),bla SHV-99 (GenBank JSER01000008.1; 101,044-101,904 bp),bla CTX-M-2 (GenBank JSER01000045.1; 2,516-3,391 bp),bla CARB-2 (GenBank JSER01000014.1; 1,461-2,375 bp),bla NDM-1 GenBank JSER01000063.1; 4,749-5,561 bp),oqxA (GenBank JSER01000088.1; 4,991-6,166 bp), oqxB(GenBank JSER01000088.1; 2,518-4,967 bp), qnrA1 (GenBank JSER01000080.1; 416-1,072 bp), catA1 (GenBank JSER01000041.1; 3,303-3,962 bp), sul1 (GenBank JSER01000058.1; 4,497-5,423 bp),tet(D) (GenBank JSER01000091.1; 392-1,576 bp) and dfrA8(GenBank JSER01000058.1; 816-1,325 bp). Overall, we observed that these NDM-1-producing Enterobacteriaceae strains carry resistance genes to different antimicrobial classes, which can explain the multidrug resistant profile observed.

In P. rettgeri CCBH 11880, the bla NDM-1 was found inside the Tn125 transposon, which is composed of two copies of ISAba125 (Carvalho-Assef et al. 2013) (data not shown). In the E. hormaechei (Carvalho-Assef et al. 2014) (GenBank acession NG041719) and K. pneumoniae isolates, thebla NDM-1 gene was found flanked by only one copy of ISAba125 (truncated) at the right boundary (data not shown).

The announcement of the whole-genome sequence of the three NDM-1-producing Enterobacteriaceae strains also provides basis for other studies, which will certainly increase our understanding of the role of this species in the drug resistance scenario.

Nucleotide sequence accessions - These Whole Genome Shotgun project have been deposited in DDBJ/ENA/GenBank under the accessions JSEQ00000000, JSBO00000000 and JSER00000000 for P. rettgerii (CCBH11880), E. hormaechei (CCBH10892) and K. pneumoniae (CCBH13327), respectively. The versions described in this paper are the first version (JSEQ00000000, JSBO00000000 and JSER00000000).


Carvalho-Assef AP, Pereira PS, Albano RM, Berião GC, Chagas TPG, Timm LN, da Silva RCF, Falci DR, Asensi MD 2013. Isolation of NDM-producingProvidencia rettgeri in Brazil. J Antimicrob Chemother 68: 2956-2957. [ Links ]

Carvalho-Assef AP, Pereira PS, Albano RM, Berião GC, Tavares CP, Chagas TP, Marques EA, Timm LN, da Silva RC, Falci DR, Asensi MD 2014. Detection of NDM-1, CTX-M-15 and qnrB4-producing Enterobacter hormaechei isolates in Brazil. Antimicrob Agents Chemother 58: 2475-2476. [ Links ]

Nordmann P, Naas T, Poirel L 2011. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 17: 1791-1798. [ Links ]

Pereira PS, Borghi M, Albano RM, Berião GC, Lopes JCO, Asensi MD, Carvalho-Assef APD 2014. First description of NDM-1-producing Klebsiella pneumoniae in Brazil. 24th European Congress of Clinical Microbiology and Infectious Diseases, P1196, Poster Session V, Worldwide spread of carbapenem resistance. Available from: [ Links ]

Pillonetto M, Arend L, Vespero EC, Pelisson M, Chagas TP, Carvalho-Assef AP, Asensi MD 2014. The first report of NDM-1-producingAcinetobacter baumannii ST 25 in Brazil. Antimicrob Agents Chemother 58: 7592-7594. [ Links ]

Rozales FP, Ribeiro VB, Magagnin CM, Pagano M, Lutz L, Falci DR, Machado A, Barth AL, Zavascki AP 2014. Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil. Int J Infect Dis 25: 79-81. [ Links ]

Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, Walsh TR 2009. Characterization of a new metallo-β-lactamase gene,bla NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniaesequence type 14 from India. Antimicrob Agents Chemother 53: 5046-5054. [ Links ]

Financial support: CNPq, FIOCRUZ

Received: February 27, 2015; Accepted: April 27, 2015

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