Acessibilidade / Reportar erro

Adamantylidene-substituted alkylphosphocholine TCAN26 is more active against Sporothrix schenckii than miltefosine

Abstract

Sporotrichosis is the most frequent subcutaneous mycosis in the world and its increasing incidence has led to the search for new therapeutic options for its treatment. In this study, we demonstrated that three structural analogues of miltefosine (TCAN26, TC19, and TC70) showed inhibitory activity against Sporothrix schenckii sensu stricto and that TCAN26 was more active in vitro than miltefosine against several isolates. Scanning electron microscopy showed that S. schenckii exposure to TCAN26 resulted in cells that were slightly more elongated than untreated cells. Transmission electron microscopy showed that TCAN26 treatment induced loss of the regular cytoplasmic electron-density and altered the cell envelope (disruption of the cell membrane and cell wall, and increased cell wall thickness). Additionally, TCAN26 concentrations required to kill S. schenckii cells were lower than concentrations that were cytotoxic in mammalian cells, and TCAN26 was more selective than miltefosine. Thus, the adamantylidene-substituted alkylphosphocholine TCAN26 is a promising molecule for the development of novel antifungal compounds, although further investigations are required to elucidate the mode of action of TCAN26 in S. schenckii cells.

Sporothrix schenckii; antifungal; miltefosine analogue


Sporotrichosis has emerged as the most frequent subcutaneous mycosis in the world (Chakrabarti et al. 2015Chakrabarti A, Bonifaz A, Gutierrez-Galhardo MC, Mochizuki T, Li S. Global epidemiology of sporotrichosis. Med Mycol. 2015; 53(1): 3-14.), and has become a major public health problem in Brazil (Freitas et al. 2014Freitas DF, Valle AC, da Silva MB, Campos DP, Lyra MR, de Souza RV, et al. Sporotrichosis: an emerging neglected opportunistic infection in HIV-infected patients in Rio de Janeiro, Brazil. PLoS Negl Trop Dis. 2014; 8(8): e3110.). It is caused by Sporothrix spp., of which Sporothrix schenckii sensu stricto is one of the most virulent species that is frequently isolated from human cases globally (Zhang et al. 2015Zhang Y, Hagen F, Stielow B, Rodrigues AM, Samerpitak K, Zhou X, et al. Phylogeography and evolutionary patterns in Sporothrix spanning more than 14,000 human and animal cases reports. Persoonia. 2015; 35(1): 1-20.). Despite being a disease with an increasing incidence worldwide (Chakrabarti et al. 2015Chakrabarti A, Bonifaz A, Gutierrez-Galhardo MC, Mochizuki T, Li S. Global epidemiology of sporotrichosis. Med Mycol. 2015; 53(1): 3-14.), there are few therapeutic options available for sporotrichosis treatment. While itraconazole is the ‘gold standard’ treatment for the cutaneous and lymphocutaneous forms, amphotericin B is the first-line drug of choice for disseminated forms of the disease (Kauffman et al. 2007Kauffman CA, Bustamante B, Chapman SW, Pappas PG, Infectious Diseases Society of America. Clinical practice guidelines for the management of sporotrichosis: 2007 update by the Infectious Diseases Society of America. Clin Infect Dis. 2007; 45(10): 1255-65.). However, itraconazole therapy involves prolonged treatment and is expensive, and amphotericin B therapy induces several side effects. Therefore, search for new therapeutic options for sporotrichosis treatment is necessary.

Recently, the antifungal activity of miltefosine, an alkylphospholipid analogue, was described against S. schenckii complex species (Brilhante et al. 2014Brilhante RSN, Malaquias ADM, Caetano EP, Castelo-Branco DS, Lima RA, Marques FJF, et al. In vitro inhibitory effect of miltefosine against strains of Histoplasma capsulatum var. capsulatum and Sporothrix spp. Med Mycol. 2014; 52(3): 320-5., Borba-Santos et al. 2015Borba-Santos LP, Gagini T, Ishida K, de Souza W, Rozental R. Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole. J Med Microbiol. 2015; 64(4): 415-22.). Miltefosine’s cytotoxicity, although considerable, is comparable to that of amphotericin B (Borba-Santos et al. 2015Borba-Santos LP, Gagini T, Ishida K, de Souza W, Rozental R. Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole. J Med Microbiol. 2015; 64(4): 415-22.). Efforts to reduce miltefosine cytotoxicity have led to the synthesis of less toxic and more active alkylphospholipid analogues based on miltefosine’s structure (Avlonitis et al. 2003Avlonitis N, Lekka E, Detsi A, Koufaki M, Calogeropoulou T, Scoulica E, et al. Antileishmanial ring-substituted ether phospholipid. J Med Chem. 2003; 46(5): 755-67., Calogeropoulou et al. 2008Calogeropoulou T, Angelou P, Detsi A, Fragiadaki I, Scoulica E. Design and synthesis of potent antileishmanial cycloalkylidene-substituted the phospholipid derivatives. J Med Chem. 2008; 51(4): 897-908., Papanastasiou et al. 2010Papanastasiou I, Prousis KC, Georgikopoulou K, Pavlidis T, Scoulica E, Kolocouris N, et al. Design and synthesis of new adamantyl-substituted antileishmanial ether phospholipids. Bioorg Med Chem Lett. 2010; 20(18): 5484-7.). The purpose of this study was to verify the inhibitory activity of eight structural analogues of miltefosine against S. schenckii sensu stricto, and evaluate the antifungal activity and selectivity of the most active compound, in comparison with those of miltefosine.

Therefore, we used 12 human isolates of S. schenckii sensu stricto (described from here only as S. schenckii) to evaluate the anti-Sporothrix activity of eight structural analogues of miltefosine (Fig. 1): four reference strains (ATCC MYA 4820, ATCC MYA 4821, ATCC 32286, ATCC 16345) and eight clinical isolates (SB02, BH1, Ss 03, Ss 22, Ss 59, Ss 73, Ss 116, Ss 144) (Borba-Santos et al. 2016Borba-Santos LP, Visbal G, Gagini T, Rodrigues AM, de Camargo ZP, Lopes-Bezerra LM, et al. Δ(24)-sterol methyltransferase plays an important role in the growth and development of Sporothrix schenckii and Sporothrix brasiliensis. Front Microbiol. 2016; 311(7): 1-13.).

Fig. 1
: structures of structural analogues of miltefosine tested in the present study.

The isolates were maintained in potato dextrose agar (PDA; Difco, Detroit, USA) at 4ºC. Before the experiments, the isolates were cultivated in PDA medium at 35ºC for seven days, to obtain the filamentous form, or in brain heart infusion (BHI; Difco, Detroit, USA) broth supplemented with 2% glucose at 36ºC with orbital agitation (150 rpm) to obtain the yeast phase. The structural analogues of miltefosine were diluted in DMSO:ethanol (1:1) to obtain stock solutions of 50 mM. TCAN26 and TC19 were synthesized as previously described (Avlonitis et al. 2003Avlonitis N, Lekka E, Detsi A, Koufaki M, Calogeropoulou T, Scoulica E, et al. Antileishmanial ring-substituted ether phospholipid. J Med Chem. 2003; 46(5): 755-67., Calogeropoulou et al. 2008Calogeropoulou T, Angelou P, Detsi A, Fragiadaki I, Scoulica E. Design and synthesis of potent antileishmanial cycloalkylidene-substituted the phospholipid derivatives. J Med Chem. 2008; 51(4): 897-908.), while the synthesis of other compounds will be reported hereafter. Miltefosine (Cayman Chemical Company, MI, USA) was used as a control and was diluted in distilled water to obtain stock solutions of 2 mg/mL, maintained at -20ºC.

Antifungal activity of compounds was evaluated according to minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs). MIC values were obtained for S. schenckii using a broth microdilution method adapted from M27-A3 (yeast) or M38-A2 (filamentous form) documents of Clinical and Laboratory Standards Institute (CLSI 2008aCLSI - Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts - M27-A3. 3nd ed. Wayne: Clinical and Laboratory Standards Institute; 2008a., bCLSI - Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi - M38-A2. 2nd ed. Wayne: Clinical and Laboratory Standards Institute; 2008b.), with the following minor modifications: (i) RPMI 1640 medium was supplemented with 2% glucose and (ii) incubation time was extended to five days. Yeast (0.5-2.5 x 103 CFU/mL) or conidial suspension (0.5-2.5 x 104 CFU/mL) was treated with miltefosine analogues or miltefosine for five days at 35ºC in a dark, humid chamber with 5% CO2. MIC was defined as the lowest concentration that prevents visible fungal growth in an inverted optical microscope. MFC values were obtained by plating, in drug-free PDA, 10 µL aliquots collected for the inhibitory concentrations (at the end of the five-day incubation period), and incubating the aliquots at 35ºC for seven days. MFC was defined as the lowest drug concentration that failed to yield fungal growth. When MFC ≤ 4 x MIC, the agent is considered to be fungicidal, whereas MFC > 4 x MIC indicates fungistatic activity (Pfaller et al. 2004Pfaller MA, Sheehan DJ, Rex JH. Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clin Microbiol Rev. 2004; 17(2): 268-80.). Statistical analysis was performed using Graph Pad Prism 5.0 (Graph Pad Software Inc., USA), wherein Mann Whitney’s test was applied to analyse differences in susceptibility between the filamentous form and yeast, and correlations between MIC values were analysed by linear regression test. Statistically significance was set at p < 0.05 and positive correlation was considered if r > 0.5 and p < 0.05.

S. schenckii ATCC MYA 4821 - strain of the Genome Project (Teixeira et al. 2014Teixeira MM, de Almeida LG, Kubitschek-Barreira P, Alves FL, Kioshima ES, Abadio AK, et al. Comparative genomics of the major fungal agents of human and animal sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis. BMC Genomics. 2014; 943(15): 1-22.) - was used to perform the electron microscopy analyses. Yeasts were treated with a sub-inhibitory concentration (1/4 MIC) of the most active miltefosine analogue, for 24 h at 35ºC. Untreated and treated cells were washed three times in PBS and fixed in a solution of 2.5% glutaraldehyde and 4% formaldehyde in 0.1 M cacodylate buffer (pH 7.2) for 24 h at 4ºC. For scanning electron microscopy (SEM) visualisation, cells were plated on to poly-L-lysine-covered glass coverslips and post-fixed in 1% osmium tetroxide in 0.1 M cacodylate buffer containing 1.25% potassium ferrocyanide and 5 mM CaCl2 for 30 min. They were then washed and dehydrated in increasing ethanol concentrations ranging from standard to ultra-dry ethanol, critical-point-dried in CO2 and coated with gold. The images were obtained using FEI Quanta 250 scanning electron microscope (FEI, Netherland). Feret diameters (average of distances between two parallel lines tangential to the particle projection) of 50 cells exhibiting yeast morphology were measured using Image J software (NHI, USA) and cell sizes were estimated according these values. Aspect ratio (ratio between maximum and minimum Feret diameters) was calculated to reflect the shape of cells. Values closer to one indicate a globose/oval morphology. Values much higher or smaller than one indicate an elongated cell morphology. Differences in cell size were analysed using Mann Whitney’s test and p < 0.05 was considered statistically significant (Graph Pad Software Inc., USA). For transmission electron microscopy (TEM), cells were post-fixed in 1% osmium tetroxide in 0.1 M cacodylate buffer, containing 1.25% potassium ferrocyanide and 5 mM CaCl2, for 2 h at 4ºC, dehydrated in ethanol, and embedded in Spurr resin. Ultrathin sections were stained with uranyl acetate and lead citrate and observed under Zeiss 900 transmission electron microscope (Zeiss, Germany). Thickness of cell walls was measured using Image J software in 50 cells per sample. Differences in cell wall thickness were analysed by Mann Whitney’s test, with p < 0.05 considered statistically significant (Graph Pad Software Inc., USA).

To determine the selectivity of the most active miltefosine analogue toward S. schenckii, cytotoxicity assays were performed on mammalian cells [monkey epithelial cells from kidney (LLC-MK2)] and human erythrocytes, using concentrations of 1-100 µg/mL as described previously (Borba-Santos et al. 2016Borba-Santos LP, Visbal G, Gagini T, Rodrigues AM, de Camargo ZP, Lopes-Bezerra LM, et al. Δ(24)-sterol methyltransferase plays an important role in the growth and development of Sporothrix schenckii and Sporothrix brasiliensis. Front Microbiol. 2016; 311(7): 1-13.), and the results were compared with those obtained for miltefosine. CC50 (TCAN26 concentration that impaired viability in 50% of LLC-MK2 cells) and HA50 (TCAN26 concentration that caused lysis in 50% of erythrocytes) values were determined and the antifungal drugs’ selectivity indexes (SI) were obtained using the following equation: SI = CC50 or HA50/MIC medians.

Among all miltefosine analogues tested against the reference strain S. schenckii ATCC MYA 4821 (Fig. 1), only three (TCAN26, TC19 and TC70) showed considerable inhibitory activity (MIC < 16 µg/mL). The adamantylidene-substituted alkylphosphocholine, TCAN26, was found to be the most active compound against both yeast (MIC = 0.5 µg/mL) and filamentous forms (MIC = 1.0 µg/mL) (Table I). Therefore, TCAN26 was chosen to expand the susceptibility analyses to other isolates (including three reference strains and eight clinical isolates) (Table II, Supplementary Table).

TABLE I
Minimum inhibitory concentration (MIC) of eight structural analogues of miltefosine, compared to miltefosine, against Sporothrix schenckii ATCC MYA 4821 isolate in filamentous and yeast forms (µg/mL)

TABLE II
Antifungal activity of TCAN26, compared to that of miltefosine, against Sporothrix schenckii isolates in filamentous and yeast forms (µg/mL)

According to MIC values, TCAN26 was more active than miltefosine (MIC median equal to 1 and 2 μg/mL, respectively) against S. schenckii yeasts (p = 0.0187) and the filamentous form (p = 0.003). MIC values for TCAN26 ranged from 0.25 to 2 µg/mL for yeasts and from 0.5 to 1 µg/mL for filamentous forms, whereas MIC values for miltefosine ranged from 0.5 to 2 µg/mL for yeasts and filamentous forms (Table II). No statistically significant differences were observed between MIC values of TCAN26 for filamentous form and yeasts (p = 0.8408); similar findings were observed for miltefosine (p = 0.5732). Positive correlations were obtained for MIC values in filamentous and yeast forms for TCAN26 (r = 0.7588 and p = 0.0026) and miltefosine (r = 0.6721 and p = 0.0119). These data indicate that both forms were equally sensitive to TCAN26 and miltefosine. MFC values for TCAN26 ranged from 0.5 to 4 µg/mL for yeasts and from 0.5 to 8 µg/mL for filamentous forms, whereas MFC values for miltefosine ranged from 1 to 4 µg/mL for yeasts and from 1 to 16 µg/mL for filamentous forms. Additionally, MFC values suggested that TCAN26, similar to miltefosine, showed fungicidal activity (MFC ≤ 4 x MIC) (Table II, Supplementary Table).

To assess ultrastructural alterations after exposure to TCAN26, yeasts of the reference isolate ATCC MYA 4821 were treated with 0.125 µg/mL TCAN26 (1/4 MIC), for 24 h, and processed by SEM and TEM. SEM images revealed that the control sample exhibited yeast cells with an elongated morphology and some hyphae cells (white arrow in Fig. 2A), and TCAN26 exposure did not induce pronounced superficial changes in cells (Fig. 2C). Feret diameter analyses indicated no alterations in yeast size after TCAN26 treatment (p > 0.05) (hyphae and undetermined cells were disregarded for this analysis), but yeasts treated with TCAN26 were slightly more elongated than control yeasts (aspect ratio mean equal to 3.33 ± 0.16 and 3.13 ± 0.14, respectively). TEM images showed that control cells exhibited homogenous and electron-dense cytoplasm containing nucleus (N), vacuoles (v) and several mitochondria (m), and surrounded by cell membrane and cell wall (Fig. 2B and inset). TCAN26 exposure reduced cytoplasmic electron-density (Fig. 2D) and induced disruption of the cell membrane and cell wall (arrowheads in Fig. 2D). The cell wall of TCAN26-treated cells (mean of 163.7 nm) was thicker than that of control cells (mean of 142.2 nm) (p = 0.0006).

Fig. 2
: ultrastructural alterations of Sporothrix schenckii ATCC MYA 4821 on exposure to TCAN26, evaluated by scanning electron microscopy (A, C) and transmission electron microscopy (B, D). Control cells (untreated) exhibit yeasts and some hyphae cells (Fig. 2A, white arrow) while samples treated with 0.125 µg/mL TCAN26 for 24 h show only yeasts (Fig. 2C). Control cells exhibit homogenous and electron-dense cytoplasm containing nucleus (N), vacuoles (v), and several mitochondria (m), and are surrounded by the cell membrane and cell wall (Fig 2B and inset). TCAN26 exposure-induced reduction of cytoplasmic electron-density (Fig. 2D), disruption of cell membrane and cell wall (arrowheads in Fig. 2D) and increase in the cell wall thickness (inset in Fig. 2D) (Bars: A, C: 5 µm; B, D: 1 µm; insets in B and D: 0.5 µm).

Selectivity of TCAN26 towards S. schenckii was evaluated according to CC50 and HA50 values determined in LLC-MK2 cells and human erythrocytes, respectively. TCAN26 was 23 times more selective for fungus than for LLC-MK2 cells and 52 times more selective for fungus than for erythrocytes. TCAN26 was also more selective for fungus and less cytotoxic to mammalian cells than miltefosine was (Table III).

TABLE III
Selectivity of TCAN26, compared to that of miltefosine, for Sporothrix schenckii

Currently, sporotrichosis is the major subcutaneous fungal infection (Chakrabarti et al. 2015Chakrabarti A, Bonifaz A, Gutierrez-Galhardo MC, Mochizuki T, Li S. Global epidemiology of sporotrichosis. Med Mycol. 2015; 53(1): 3-14.). The availability of only a few therapeutic options warrants a search for more effective antifungal agents. Here, we demonstrated that three structural analogues of miltefosine (TCAN26, TC19 and TC70) inhibited fungal growth and TCAN26 was more selective than miltefosine against S. schenckii.

The antimicrobial activity of TCAN26 was previously reported in Leishmania spp. with its inhibitory activity ranging from 1 to 20 µg/mL, depending on the species (Calogeropoulou et al. 2008Calogeropoulou T, Angelou P, Detsi A, Fragiadaki I, Scoulica E. Design and synthesis of potent antileishmanial cycloalkylidene-substituted the phospholipid derivatives. J Med Chem. 2008; 51(4): 897-908.). TCAN26 reportedly also showed inhibitory activity against Candida albicans yeasts (MIC equal to 4 µg/mL) and biofilms (Vila et al. 2013Vila TCM, Ishida K, de Souza W, Prousis K, Calogeropoulou T, Rozental S. Effect of alkylphospholipids on Candida albicans biofilm formation and maturation. J Antimicrob Chemother. 2013; 68(1): 113-25.). Interestingly, our results showed that TCAN26 was more active against S. schenckii than it was against previously reported microorganisms, impairing fungal growth with concentrations ranging from 0.25 to 2 µg/mL. In addition, TCAN26 showed a fungicidal activity profile against S. schenckii (Table II, Supplementary Table).

In this work, we also demonstrated that TCAN26 was more selective for fungal cells (Table III) than for mammalian cells (23 times more selective than for LLC-MK2 cells and 52 times more selective than for human erythrocytes). Considering fungal MFC, the TCAN26 concentrations required to kill S. schenckii cells (≤ 8 µg/mL) were lower than the concentrations cytotoxic to LLC-MK2 cells (CC50 = 23 µg/mL) and lower than the concentrations that caused lysis in erythrocytes (HA50 = 52 µg/mL).

Being an alkylphospholipid analogue, TCAN26 probably alters cellular lipid homeostasis in a manner similar to that observed with miltefosine (Marco et al. 2014Marco C, Ríos-Marco P, Jiménez-López JM, Segovia JL, Carrasco MP. Antitumoral alkylphospholipids alter cell lipid metabolism. Anticancer Agents Med Chem. 2014; 14(4): 545-58.). S. schenckii exposure to TCAN26 resulted in cells that were slightly more elongated than control cells (untreated) (Fig. 2A-C), and this treatment induced loss of the regular cytoplasmic electron-density and altered the cell envelope (disruption of the cell membrane and cell wall, and increased cell wall thickness) (Fig. 2D). C. albicans treated with TCAN26 also showed cell membrane alterations and increase in cell wall thickness (Vila et al. 2013Vila TCM, Ishida K, de Souza W, Prousis K, Calogeropoulou T, Rozental S. Effect of alkylphospholipids on Candida albicans biofilm formation and maturation. J Antimicrob Chemother. 2013; 68(1): 113-25.).

In conclusion, this study suggests that the adamantylidene-substituted alkylphosphocholine TCAN26 is a promising molecule for the development of new antifungal compounds, although further investigations need to be conducted to elucidate the mode of action of TCAN26 in S. schenckii cells.

ACKNOWLEDGEMENTS

To Dr Zoilo Pires de Camargo and Dr Anderson Messias Rodrigues, from the Federal University of São Paulo, São Paulo, SP, Brazil, and Dr Leila Maria Lopes-Bezerra, from the State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil, for providing the isolates used in this work. The authors thank Beatriz Bastos Fonseca, from the Laboratory of Cell Biology of Fungi, for assistance in scanning electron microscopy experiments.

SUPPLEMENTARY TABLE


Antifungal activity of TCAN26 and miltefosine against Sporothrix schenckii isolates in filamentous and yeast forms (µg/mL)

REFERENCES

  • Avlonitis N, Lekka E, Detsi A, Koufaki M, Calogeropoulou T, Scoulica E, et al. Antileishmanial ring-substituted ether phospholipid. J Med Chem. 2003; 46(5): 755-67.
  • Borba-Santos LP, Gagini T, Ishida K, de Souza W, Rozental R. Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole. J Med Microbiol. 2015; 64(4): 415-22.
  • Borba-Santos LP, Visbal G, Gagini T, Rodrigues AM, de Camargo ZP, Lopes-Bezerra LM, et al. Δ(24)-sterol methyltransferase plays an important role in the growth and development of Sporothrix schenckii and Sporothrix brasiliensis Front Microbiol. 2016; 311(7): 1-13.
  • Brilhante RSN, Malaquias ADM, Caetano EP, Castelo-Branco DS, Lima RA, Marques FJF, et al. In vitro inhibitory effect of miltefosine against strains of Histoplasma capsulatum var. capsulatum and Sporothrix spp. Med Mycol. 2014; 52(3): 320-5.
  • Calogeropoulou T, Angelou P, Detsi A, Fragiadaki I, Scoulica E. Design and synthesis of potent antileishmanial cycloalkylidene-substituted the phospholipid derivatives. J Med Chem. 2008; 51(4): 897-908.
  • Chakrabarti A, Bonifaz A, Gutierrez-Galhardo MC, Mochizuki T, Li S. Global epidemiology of sporotrichosis. Med Mycol. 2015; 53(1): 3-14.
  • CLSI - Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts - M27-A3. 3nd ed. Wayne: Clinical and Laboratory Standards Institute; 2008a.
  • CLSI - Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi - M38-A2. 2nd ed. Wayne: Clinical and Laboratory Standards Institute; 2008b.
  • Freitas DF, Valle AC, da Silva MB, Campos DP, Lyra MR, de Souza RV, et al. Sporotrichosis: an emerging neglected opportunistic infection in HIV-infected patients in Rio de Janeiro, Brazil. PLoS Negl Trop Dis. 2014; 8(8): e3110.
  • Kauffman CA, Bustamante B, Chapman SW, Pappas PG, Infectious Diseases Society of America. Clinical practice guidelines for the management of sporotrichosis: 2007 update by the Infectious Diseases Society of America. Clin Infect Dis. 2007; 45(10): 1255-65.
  • Marco C, Ríos-Marco P, Jiménez-López JM, Segovia JL, Carrasco MP. Antitumoral alkylphospholipids alter cell lipid metabolism. Anticancer Agents Med Chem. 2014; 14(4): 545-58.
  • Papanastasiou I, Prousis KC, Georgikopoulou K, Pavlidis T, Scoulica E, Kolocouris N, et al. Design and synthesis of new adamantyl-substituted antileishmanial ether phospholipids. Bioorg Med Chem Lett. 2010; 20(18): 5484-7.
  • Pfaller MA, Sheehan DJ, Rex JH. Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clin Microbiol Rev. 2004; 17(2): 268-80.
  • Teixeira MM, de Almeida LG, Kubitschek-Barreira P, Alves FL, Kioshima ES, Abadio AK, et al. Comparative genomics of the major fungal agents of human and animal sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis BMC Genomics. 2014; 943(15): 1-22.
  • Vila TCM, Ishida K, de Souza W, Prousis K, Calogeropoulou T, Rozental S. Effect of alkylphospholipids on Candida albicans biofilm formation and maturation. J Antimicrob Chemother. 2013; 68(1): 113-25.
  • Zhang Y, Hagen F, Stielow B, Rodrigues AM, Samerpitak K, Zhou X, et al. Phylogeography and evolutionary patterns in Sporothrix spanning more than 14,000 human and animal cases reports. Persoonia. 2015; 35(1): 1-20.
  • Financial support: FAPERJ, CAPES, CNPq.

Publication Dates

  • Publication in this collection
    11 July 2016
  • Date of issue
    Aug 2016

History

  • Received
    5 Mar 2016
  • Accepted
    3 June 2016
Instituto Oswaldo Cruz, Ministério da Saúde Av. Brasil, 4365 - Pavilhão Mourisco, Manguinhos, 21040-900 Rio de Janeiro RJ Brazil, Tel.: (55 21) 2562-1222, Fax: (55 21) 2562 1220 - Rio de Janeiro - RJ - Brazil
E-mail: memorias@fiocruz.br