Introduction
Brazil has ideal climatic conditions for the production of various fruit trees, including temperate species, commonly produced in the South and Southeast (ROSA et al., 2017a). Peach tree crop (Prunus persica L.) is widely used in family farming and generates direct and indirect jobs in both industry and commerce (GONÇALVES et al., 2019). The productivity and quality of the fruits may be related to the production of quality seedlings (MAYER et al., 2014), being the quality of the rootstock a limiting factor for the high vigor seedlings production and fruit yield (ROSA et al., 2017b).
The peach tree is propagated by grafting with the scion cultivar over the rootstock from seeds, however, the use of seeds increases genetic variability among the seedlings, making the propagation of the rootstocks by cuttings a viable practice, leading to production of clonal uniform seedlings with the same characteristics to the stock plant (TOMAZ et al., 2016). In addition to avoiding genetic variability, propagation of the rootstock by cuttings is viable because it is easy to perform and reduces the time required for seedling production (ROSA et al., 2017b).
Vegetative propagation by cuttings is made by the removal of parts of the stock plant to make a new complete plant by adventitious rooting induction (FERRIANI et al., 2008). This technique allows the shortly obtentions of commercial orchards with high genetic potential, because it ensure that the stock plant features are maintained, generating uniformity, precocity and high fruit quality (LATTUADA et al., 2011).
The rooting capacity of the cuttings can be increase with the use of PGR (Plant Growth Regulators) (TIMM et al., 2015). Auxin is an endogenous PGR that has an important function in rooting, but, depending on the physiologic or genetic conditions of the cuts and on the period of the year the levels of auxin can be low or scarce, being necessary exogenous auxins application, like indolbutyric acid (IBA) (PIZZATTO et al., 2011).
The part of the plant that is used is also a factor to be considered on peach tree propagation, once herbaceous cuttings have a smaller lignification degree, that allows the transport of substances, plus, herbaceous cuttings have high levels of auxin, favoring the rooting and development of the cuttings (ROSA et al., 2017b).
The rootstock influence on yield, on characters of the scion cultivar and also on the adoption of cultural practices like density, pruning, thinning and harvest, reason why the study of different genotypes of peach becomes extremely important (SCHMITZ et al., 2012).
Based on the presented, the aim of this study was to evaluate the initial development of different genotypes of peach rootstock propagated by herbaceous cuttings.
The experiment was conducted in a greenhouse located in Teaching, Research and Extension Unit of Federal University of Viçosa (20°45’26’’ South Latitude and 42°52’08’’ West Longitude and 638 m of altitude).
The region climate is of the type Cwa, with annual average temperature of 19°C and rainfall index of 1,314 mm per year.
For the propagation of rootstocks of peach (Prunus persica L.), were used 15 cm length apical cuttings without the apical meristem and with a couple of leaves, treated with indolbutyric acid (IBA) at 3,000 mg L-1 concentration for five seconds, which had shown to be efficient in preliminary studies. The cuttings were collected from a single stock plant for each genotype in winter season, obtained from the middle and apical portions of the plant and wrapped in plastic boxes (36.5 x 26.5 x 9.5 cm) with sand, with a depth of 4 to 5 cm and kept on intermittent fogging for 49 days, with timer programmed to be triggered for 5 seconds, in time interval of 3.5 minutes.
After the rooting, were transplanted to polyethylene bags with capacity for 3 L and the dimensions of 30 x 25 x 20 cm, with a mixture of ravine soil, washed sand and organic matter (tanned corral manure) in volumetric proportion (2:1:1). It was made a specific fertilization for seedlings production (Osmocote®), 30 days after transplant, in the dosage of 6 g/plastic bag, of formulation 15-10-10 + micronutrients.
The cuttings were selected based on the selection index of Mulamba and Mock (1978), according to results obtained in a previous study (OLIVEIRA et al., 2018).
Plants were conducted in single rod, being staking with bamboo stakes of 70 cm length, which were fixed on substrate near to rootstock and tied with cotton string, when required.
The experimental design used was the completely randomized, with five repetitions and eight cuttings for replication, being each plot composed by three plants.
Were tested 16 genotypes of peach: 713-3, 713-6, 713- 7, 713-10, 813-2, 813-3, 813-5, 813-15, 913-3, 913-6, 913-11, 913-17, C6, C7 and C8 from the UFV breeding program.
After 80 days of the cuttings transplant, the parameter was evaluated: stem length (measuring since the insertion on the original cutting to the apical meristem), whose results were expressed in cm, stem diameter, expressed in mm (measured at 5 cm of the original cutting) and length of internodes, expressed in cm.
The data were submitted to variance analysis by the test F, and the averages were compared by test of Scott- Knott, at level of 5% of probability, through the statistical software GENES (CRUZ, 2016).
Among the evaluated rootstock genotypes, 713-10, 713-13 and 813-5 had the longest stem length and 1113- 11 had reduced length (Figure 1A). Genotypes 713-13 and 813-5 showed an increase in stem diameter, while genotype 1113-11 presented a smaller diameter, indicating that the rootstock may be less vigorous (Figure 1B). The internode length of peach cuttings was higher in genotypes 713-13 and 813-5 and the shortest lengths were observed According to Galarça et al. (2013), plants with reduced length may have decreased productivity, however, there is the possibility of density, which would compensate for the decrease in productivity. Also, according to these authors, for both peach and citrus crops, it is possible to observe that smaller rootstocks showed good yield results.

Figure 1 Stem length (A), Stem diameter (B) and internode length (C) from seedlings of 16 peach rootstock genotypes from herbaceous cuttings.
The rootstock is capable of providing several characteristics that are absent in scion cultivar, such as resistance to pests and diseases, adaptability to climatic conditions and tolerance to saline or flooded soils, as well as modify the development of the scion cultivar decreasing crown size, which allows the establishment of orchards with high plant density (GAINZA et al., 2015). Rootstocks with these agronomic characteristics, especially the small size of the plant, are highly desirable in peach culture (SCHMITZ et al., 2014). The breeding program of the Federal University of Viçosa works to provide new options for peach rootstocks adapted to the growing region and with good interaction with the cultivar-canopy.
The density with the use of less vigorous rootstocks in peach tree contributes significantly to reduce and/or solve the problem of seasonality, low productivity and high perishability that peach tree culture faces, since among the peach tree producing countries, Brazil occupies the 21st position. The reduction in internode length induces an increase in the number of flowers and fruits in the productive branches, a fact probably caused by the lower vegetative growth, which consequently leads to less competition between leaves and fruits (TOMAZ et al., 2010). The initial growth of peach cuttings is influenced by a combination of factors such as the nutrient absorption capacity of the soil and the length and volume of the root system (OLDONI et al., 2019).
Genotype 1113-11 presented lower initial development, which is a desirable trait for densified cultivation and the rootstocks of the genotypes 713-13 and 813-5 were the ones that showed the highest initial development.