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CHEMICAL PROFILES OF ESSENTIAL OILS AND FATTY ACIDS FROM Erythrina velutina

Abstract

Chemical analysis involving gas chromatography coupled to mass spectrometry of the essential oils of red and orange flowers from E. velutina revealed a predominance of phenylpropanoids in both samples. However, qualitative and quantitative differences were found in the compositions. (E)-Asarone (16.22 ± 0.23%) and (E)-caryophyllene (12.22 ± 0.11%) were identified as the major constituents of the essential oil from the red flowers, whereas (E)-caryophyllene (41.72 ± 1.65%) and eugenol (20.99 ± 0.21%) were identified as the major constituents of the oil from the orange flowers. Seventeen fatty acids were characterized in the red and black seeds. The fatty acid composition in the two chromatic variations had a high degree of similarity. Oleic acid (41.56 ± 1.33% and 46.12 ± 1.90% in the red and black seeds, respectively), palmitic acid (15.39 ± 0.16% and 12.33 ± 0.41%) and behenic acid (9.95 ± 0.14% and 12.10 ± 0.42%) were identified as the main fatty acids. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) enabled identifying significant differences in the chemical profile of the essential oils from the red flower and orange flower varieties of E. velutina. In contrast, PCA and HCA analysis revealed no significant differences in the fatty acid profile of the red and black seeds.

Keywords:
Erythrina velutina; fatty acids; essential oil; chemical constituents


INTRODUCTION

The genus Erythrina (Fabaceae) has broad distribution in tropical and subtropical regions and is composed of 120 species, 70 of which are found in the Americas.11 Schrire, B. D. In Legumes of the world; Lewis, G., Schrire, B. D., Mackinder, B., Lock, M., eds.; Royal Botanic Gardens: Kew, 2005, pp. 393-431. Eleven species of the genus occur in Brazil.22 http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB22965, accessed in October 2018.
http://floradobrasil.jbrj.gov.br/jabot/f...
Erythrina velutina is particularly abundant in the state of Pernambuco, occurring on both the mainland and the island of Fernando de Noronha. Known locally as “mulungu”, E. velutina is a large arboreal plant with a twisted stem that is employed for landscaping purposes and the regeneration of degraded areas. In folk medicine, the infusion of the bark is indicated as a tranquilizer and cough suppressant as well as for the treatment of bronchitis, hemorrhoids and parasitic intestinal infections.33 Lorenzi, H.; Árvores brasileiras: Manual de Identificação e Cultivo de Plantas arbóreas nativas do Brasil, Editora Plantarum: São Paulo, 1992.,44 Agra, M. F.; Silva, K. N.; Basílio, I. J. L. D.; França, P. F.; Barbosa-Filho, J. M.; Rev. Bras. Farmacogn. 2008, 18, 472.

The flowers of E. velutina are predominantly red, with some plants exhibiting orange flowers. However, other plants, especially cultivated ones, have different colors, such as greenish yellow, white or pale.55 Neill, D. A. In Erythrina in the New and Old Worlds; Westley, S. B., Powell, M. H., eds.; Nitrogen Fixing Tree Research Reports: Hawaii, 1993, cap. 1. The fruit is a dehiscent legume, with a pod measuring 5 to 8 cm in length containing one to three kidney-shaped, shiny red seeds.66 Lorenzi, H.; Matos, F. J. A.; Plantas Medicinais no Brasil: Nativas e Exóticas, 2nd ed., Nova Odessa/Plantarum: São Paulo, 2008. Ridley77 Ridley, H. N.; Bot. J. Linn. Soc. 1890, 27, 95. identified a plant found on the island of Fernando de Noronha with the same morphological characteristics as E. velutina, but with chromatic differences in the seeds as a new species denominated E. aurantiaca (black or blackish red seeds). In the mid 20th century, Krukoff88 Krukoff, B. A.; Brittonia 1939, 3, 205. noted that the species Ridley identified had many similarities to E. velutina, with a difference only in the color of the seeds. As E. aurantiaca had blackish red or black seeds and E. velutina had red seeds, Krukoff proposed a new combination: E. velutinafo. aurantiaca. Recently, genetic studies of individuals with red and black or blackish red seeds collected on the island of Fernando de Noronha demonstrated similarities and proposed the synonymy of the aurantiaca form of Erythrinavelutina.99 Martins, M. V.; Tese de Doutorado, Universidade estadual de Campinas, Brasil, 2014. Specimens with orange flowers and black or blackish red seeds only occur on the island of Fernando de Noronha. According to a bibliographic survey, chemical investigations of E. velutina (red seeds) collected on the mainland reveal the presence of alkaloids and flavonoids.1010 Da Cunha, E. V. L.; Dias, C.; Barbosa-Filho, J. M.; Gray, A. I.; Phytochemistry 1996, 43, 1371.,1111 Rabelo, L. A.; Agra, M. F.; Cunha, E. V. L.; Silva, M. S.; Barbosa-Filho. J. M.; Biochem. Syst. Ecol. 2001, 29, 543. However, no previous study has been conducted for the identification of the chemical constituents of the essential oils from this species.

In our ongoing efforts to broaden knowledge on the chemical profiles of plants that occur in the state of Pernambuco (northeastern Brazil), the aim of the present study was to determine and compare the chemical composition of the essential oils from the flowers and fatty acids from the seeds of E. veluntina that occur on the island of Fernando de Noronha and have chromatic differences in the seeds and flowers.

MATERIAL AND METHODS

Collection of plant material

The flowers and seeds of each synonym of Erythrina velutina Willd. were collected from five specimens from different sites (light orange flower and black seeds: Forte de Nossa Senhora dos Remédios 32º24’37” W 3º50’22” S; Praia da Conceição 32º25’08” W 3º50’50” S; Praia do Sancho 32º25’59” W 3º51’18” S; Alameda do Boldró 32º25’05” W 3º50’58” S and Açude da Ema 32º25’42” W 3º51’01” S; red flowers and red seeds: Forte de Nossa Senhora dos Remédios 32º24’37” W 3º50’22” S; Praia do Sancho 32º25’58” W 3º51’18” S and 32º25’59” W 3º51’18” S; Alameda do Boldró 32º25’05” W 3º50’58” S; Açude da Ema 32º25’45” W 3º51’04” S) on the Fernando de Noronha archipelago in the state of Pernambuco - Brazil. The plants were identified by botanist M. R. Pereira (Federal Rural University of Pernambuco). Voucher of both samples were mounted and deposited in the Vasconcelos Sobrinho Herbarium of the same university (UFRPE) under numbers: 19951 Erythrina velutina (red seed) and 49645 E. velutina (black seed).

Chemicals

All monoterpenes (α-pinene; β-pinene; myrcene; limonene; terpinolene; camphor; α-terpineol; carvacrol), sesquiterpenes ((E)‑caryophyllene; caryophyllene oxide), phenilpropanoids (eugenol; eugenol acetate; (E)-asarone; (Z)-asarone; apiole) and methyl esters (methyl myristate (C14:0), methyl palmitate (C16:0) and methyl stearate (C18:0)) were purchased from Sigma-Aldrich - Brazil (> 98%).

HPLC-grade methanol and hexane were purchased from Merck SA and used without further purification. Hydrochloric acid (36%), anhydrous sodium sulfate (Na2SO4) and potassium hydroxide (KOH, > 85%) were purchased from Sigma-Aldrich - Brazil. Deionized water was purified by a Milli-Q purification system (Millipore, Bedford, MA, USA).

Isolation of essential oils

The essential oils from fresh red and orange flowers (200 g) were separately isolated using a modified Clevenger-type apparatus and hydrodistillation for 2 h. The oil layers were separated and dried over anhydrous sodium sulfate and stored in hermetically sealed glass containers at a low temperature (-5 ºC) until analysis. Total oil yields were expressed as percentages (g/100 g of fresh plant material). All experiments were carried out in triplicate.

Preparation of fatty acid methyl esters from seed oil

Approximately 50 g of E. velutina black and red seeds were milled and extracted separately with n-hexane in a Soxhlet apparatus. The residual solvent in the oil was evaporated and the oil yield was determined in relation to the dry mass of the original sample. The oils from the black and red E. velutina seeds were converted into methyl esters using the transesterification process in methanol with a basic catalyst. The dried oils reacted with the catalyst (potassium hydroxide) dissolved in methanol for 45 min at 45 ºC.1212 Fernandes, A. M. A. P.; Khatib, E. S.; Cunha, I. B. S.; Porcari, A. M.; Eberlin, M. N.; Silva, M. J.; Silva, P. R.; Cunha, V. S.; Daroda, R. J.; Alberici, R. M.; Energy Fuel 2015, 29, 3096. A solution of hydrochloric acid was added, followed by water until reaching pH 7. The methyl esters were extracted three times with n-hexane. The organic phases were united and the solvent was removed with a rotary evaporator. An aliquot corresponding to about 0.1 mg was diluted in 1 mL of n-hexane and analyzed by gas chromatography coupled to mass spectrometry (GC-MS) to determine the fatty acid composition.

Gas chromatography

Quantitative GC analyses were carried out using a Hewlett-Packard 5890 Series II GC apparatus equipped with a flame ionization detector (FID) and a non-polar DB-5 fused silica capillary column (30 m x 0.25 mm x 0.25 µm) (J & W Scientific). The oven temperature was programmed from 60 to 240 ºC and held for 6 min at a rate 3 ºC min-1. Injector and detector temperatures were 260 ºC. Hydrogen was the carrier gas, with a flow rate of 1 mL min-1 in split mode (1:30). The injection volume was 0.5 µL of diluted solution (1/100) of oil in n-hexane. The amount of each compound was calculated from GC peak areas in the order of the DB-5 column elution and expressed as a relative percentage of the area of the chromatograms. All analyses were carried out in triplicate.

Gas chromatography-mass spectrometry

The GC-MS analysis was carried out using a Varian 220-MS IT GC system with a mass selective detector mass spectrometer in EI 70 eV with a scan interval of 0.5 s and fragments from 40 to 550 Da fitted with the same column and temperature program as that for the GC experiments. Helium was the carrier gas, with a flow rate of 1 mL min-1 in split mode (1:30). The injection volume was 1 µL of diluted solutions (1/100) of oil in n-hexane. All analyses were carried out in triplicate.

Identification of components

The identification of the components was based on GC retention indices with reference to a homologous series of C8-C40 n-alkanes calculated using the Van den Dool and Kratz equation1313 Van D. L. H.; Kratz, P. H.; J. Chromatogr. A 1963, 11, 463. by computer matching against the mass spectral library of the GC-MS databases, such as the NIST electron ionization (EI) mass spectral library,1414 Johnson, S.; NIST Standard Reference Database 1A: Mass Spectral Library, version 14; National Institute of Standards and Technology: Gaithersburg, MD, 2016. and co-injection with authentic standards as well as other published mass spectra.1515 Adams, R. P.; Identification of Essential Oil Components by Gas Chromatography/ Quadrupole Mass Espectroscopy, 4th ed., Allured Publishing Corporation: Carol Stream, 2007. Area percentages were obtained from the GC-FID response without the use of an internal standard or correction factors.

Statistical analysis

Based on the results of chromatographic analysis of fatty acids in the red and black Erythrina velutina seeds as well as previous reports on other species of Erythrina (E. variegate, E. velutina, E. lithosperna, E. arborescens, E. stricta, E. indica 1, E. indica 2, E. indica 3, E. speciosa, E. suberosa and E. Americana),1616 Samanta, T. D.; Laskar, S.; Biosci. Biotech. Res. Asia. 2013, 10, 433; Mayworm, M. A. S.; Nascimento, A. S.; Salatino, A.; Rev. Bras. Bot. 1998, 21, 961; Tandon, S. P.; Tiwari, K. Prasad.; Gupta, A. P.; Indian Oil and Soap J. 1969, 34, 151; Singh, H.; Chawla, A. S.; Jindal, A. K.; Subbaram, M. R.; Achaya, K. T.; Indian J. Technol. 1972, 10, 115; Amir, F.; Wan, S. Y.; Yen, C. K.; Eur. J. Chem. 2011, 2, 561; Pugalenthi, M.; Vadivel, V.; Gurumoorthi, P.; Janardhanan, K.; Trop. Subtrop. Agroecosyst. 2004, 4, 107; 22. Tresina, P. S.; Mohan, V. R.; Trop. Subtrop. Agroecosyst. 2012, 15, 539; Mello, J. I. O.; Barbedo, C. J.; Salatino, A.; Figueiredo-Ribeiro, R. C. L.; Braz. Arch. Biol. Technol. 2010, 53, 889; Joshi, R.; Garg, B. D.; J. Sci. Res. 1981, 3, 11; Giral, F.; Mariel, R.; Cienc. Mex. 1959, 19, 27. a species similarity graph was generated by the distance of the correlation coefficient using hierarchical cluster analysis (HCA) with the aid of BioDiversity Pro version 2.1717 McAlece, N.; Gage, J. D. G.; Lambshead, P. J. D.; Paterson, G. L. J.; BioDiversity Professional statistics analysis software, Scottish Association for Marine Science and the Natural History Museum, London, 1997. Principal component analysis (PCA) based on the complete dataset was conducted to evaluate the fatty acid composition of the Erythrina seeds. GC-MS data were exported in ASCII format to Microsoft Excel to produce a data matrix of sample versus metabolite peaks with associated peak areas. All the analyses were performed using Unscrambler® version 9.5.1818 Unscrambler® software version 9.5, CAMO Process AS, Norway, 1996-2010.

RESULTS AND DISCUSSION

Hyrdodistillation of the red and orange flowers from the varieties of E. velutina that occur on the island of Fernando de Noronha furnished yellow and colorless essential oils, respectively. Table 1 displays the yields, specific rotation and identification of the chemical constituents of the two essential oils. A greater yield was achieved with the orange variety (0.07 ± 0.00%). The oils were levorotatory, with a specific rotation of [α]D25 = -27.4º (c=1, CH2Cl2) for the oil from the red flowers and [α]D25 = -18.0º (c=1, CH2Cl2) for the oil from the orange flowers.

Table 1
Percentage composition (% ± SD), specific rotation and yield of essential oils from red and orange flowers of Erythrina velutina

The GC-MS analysis enabled the identification of 64 compounds, representing 94.67 ± 0.81% and 95.97 ± 1.93% of the oils from the red and orange flowers, respectively (oil chromatograms in Figure 1S, Supplementary Material). Both oils are characterized by ketones, alcohols, esters, fatty acids, monoterpenes, sesquiterpenes and phenylpropanoids.

Forty-seven compounds were identified in the essential oil from the red flowers, in which phenylpropanoids constituted the predominant chemical class (47.42 ± 0.71%). (E)-Asarone (16.22 ± 0.23%) was the major constituent, followed by (E)-caryophyllene (12.22 ± 0.11%) and exalatacin (9.94 ± 0.20%). Significant percentages of other constituents were also identified, such as eugenol (6.05 ± 0.64%), g-terpinene (5.19 ± 0.41%) and (Z)-asarone (5.99 ± 0.05%). Less diversity was found in the essential oil from the orange leaves, in which 38 compounds were identified. Phenylpropanoids (47.70 ± 0.28%) were once again the predominant class, but the main constituent was the sesquiterpene (E)‑caryophyllene (41.72 ± 1.65%), followed by eugenol (20.99 ± 0.21%) and croweacin (9.76 ± 0.14%).

Regarding the terpenoids found in the oils, the red flower variety had six-fold more monoterpenes than the orange variety. In contrast (as if a form of compensation in the production of these constituents), the amount of sesquiterpenes was twofold higher in the oil from the orange flowers. The major chemical constituents identified herein are found in both oils, varying only in terms of relative percentages. For example, (E)-asarone is the major constituent of the oil from the red flowers (16.22 ± 0.23%) and is found at a much lower percentage in the oil from the orange flowers (1.50 ± 0.00%). Likewise, (E)‑caryophyllene is the major constituent of the oil from the orange flowers (41.72 ± 1.65%) and is found at a lower percentage in the oil from the red flowers (12.22 ± 0.11%). Quantitative differences were also found for other constituents present in both oils, indicating quantitative variation in the chemical profile of the oils from the red and orange varieties. Among the 64 chemical constituents identified, 24 were found in both oils, demonstrating qualitative and quantitative differences in the chemical composition of the oils investigated.

These results were confirmed by PCA, which showed a clear separation of the two oils (Figure 1). Ninety-eight percent of the variability in the data was explained by the first (PC1 = 92%) and second (PC2 = 6%) components.

Figure 1
Principal component analysis of essential oils from Erythrina velutina

To the best of our knowledge, the only study on essential oils from the genus Erythrina in the literature describes the leaf oil from E. crista-galli1919 Lamarque, A. L.; Damian M.; Maestri1, J. A. Z.; Nelson R. G.; Flavour Fragr. J. 1998, 13, 266. and no similarities are found between it and the oils described herein. The oil from E. crista-galli had monoterpenes as the predominant chemical class (70.2%), with linalool (53.9%), spathulenol (9.6%) and a-terpineol (8.4%) as the major constituents. Linalool and α-terpineol were found in the present analyses only at low percentages (1.59 ± 0.01% and 1.46 ± 0.01% in the red flower oil; 1.26 ± 0.02% and 0.00 ± 0.00% in the orange flower oil, respectively). Among the phenylpropanoids found in the red flower and orange flower oils from E. velutina, only eugenol was identified in the leaf oil from E. crista-galli (1.5%). Moreover, (E)-caryophyllene was the major constituent of the orange flower oil from E. velutina and was not found in the oil from E. crista-galli.

The fatty acids from the red (red flower variety) and black (orange flower variety) seeds of E. velutina were analyzed by GC-MS (fatty acid chromatograms in Figure 2S, Supplementary Material). Table 2 displays the chemical compositions and yields of the fatty acids found in the red and black seeds.

Table 2
Relative composition (% ± SD) and yield of fatty acids from red and black Erythrina velutina seeds

A greater yield was achieved with the red seeds (11.93 ± 0.60%), which is similar to the yield described by Rao2020 Rao, J. V. P. R.; Curr. Sci. 1945, 14, 198. for the white seeds of the white variety of E. indica (about 12% fatty acids).

A total of 13 fatty acids were identified in the two seed varieties, representing 91.17 ± 1.40% of the oil from the red seeds and 95.55 ± 1.76% of the oil from the black seeds. The oils from both colors of seeds were characterized by long-chain fatty acids ranging from 14 to 22 carbon atoms, containing saturated fatty acids (28.36 ± 0.14% for red seeds and 27.60 ± 0.09% for black seeds), monounsaturated fatty acids (52.94 ± 1.44% for red seeds and 51.78 ± 0.38% for black seeds) and polyunsaturated fatty acids (9.90 ± 0.16% for red seeds and 16.07 ± 1.94% for black seeds). The fatty acids characterized in the red and black seeds have a similar chemical profile, varying only in the relative percentages. For example, oleic acid (C18:1) is the major component of both seeds, with a significant difference in the percentage (46.12 ± 1.90% for black seeds and 41.56 ± 1.33% for red seeds). Other significant differences were found for palmitic acid (C16:0) (15.39 ± 0.36 and 12.33 ± 0.41% for red and black seeds, respectively), behenic acid (C22:0) (9.95 ± 0.14% and 12.10 ± 0.42%) and linoleic acid (C18:2) (6.13 ± 0.41% and 10.58 ± 0.52%).

The data on the fatty acid composition of the E. velutina seeds in the present study are similar to those reported by Mayworm et al.1616 Samanta, T. D.; Laskar, S.; Biosci. Biotech. Res. Asia. 2013, 10, 433; Mayworm, M. A. S.; Nascimento, A. S.; Salatino, A.; Rev. Bras. Bot. 1998, 21, 961; Tandon, S. P.; Tiwari, K. Prasad.; Gupta, A. P.; Indian Oil and Soap J. 1969, 34, 151; Singh, H.; Chawla, A. S.; Jindal, A. K.; Subbaram, M. R.; Achaya, K. T.; Indian J. Technol. 1972, 10, 115; Amir, F.; Wan, S. Y.; Yen, C. K.; Eur. J. Chem. 2011, 2, 561; Pugalenthi, M.; Vadivel, V.; Gurumoorthi, P.; Janardhanan, K.; Trop. Subtrop. Agroecosyst. 2004, 4, 107; 22. Tresina, P. S.; Mohan, V. R.; Trop. Subtrop. Agroecosyst. 2012, 15, 539; Mello, J. I. O.; Barbedo, C. J.; Salatino, A.; Figueiredo-Ribeiro, R. C. L.; Braz. Arch. Biol. Technol. 2010, 53, 889; Joshi, R.; Garg, B. D.; J. Sci. Res. 1981, 3, 11; Giral, F.; Mariel, R.; Cienc. Mex. 1959, 19, 27. for a sample collected in the state of Bahia, Brazil, with regard to the major constituents (51.0% oleic acid, 14.8% linoleic acid, 10.7% behenic acid and 10.0% palmitic acid). However, arachidic acid (2.8%) and lignoceric acid (2.6%) described in the sample from Bahia were not found in the present study.

The similarities among the fatty acids in the black and red seeds reported in this study from samples collected on the island of Fernando de Noronha and a sample collected in the state of Bahia were confirmed by hierarchical cluster analysis (HCA) and principal component analysis (PCA).

Figure 1 presents the HCA of the fatty acids identified in black and red seeds collected from Fernando de Noronha as well as data compiled from the literature on fatty acids identified in E. velutina from Bahia and other congeners from different regions of the world: E. variegata, E. lithosperna, E. arborescens, E. stricta, E. indica 1, E. indica 2, E. indica 3, E. speciosa, E. suberosa and E. americana.1616 Samanta, T. D.; Laskar, S.; Biosci. Biotech. Res. Asia. 2013, 10, 433; Mayworm, M. A. S.; Nascimento, A. S.; Salatino, A.; Rev. Bras. Bot. 1998, 21, 961; Tandon, S. P.; Tiwari, K. Prasad.; Gupta, A. P.; Indian Oil and Soap J. 1969, 34, 151; Singh, H.; Chawla, A. S.; Jindal, A. K.; Subbaram, M. R.; Achaya, K. T.; Indian J. Technol. 1972, 10, 115; Amir, F.; Wan, S. Y.; Yen, C. K.; Eur. J. Chem. 2011, 2, 561; Pugalenthi, M.; Vadivel, V.; Gurumoorthi, P.; Janardhanan, K.; Trop. Subtrop. Agroecosyst. 2004, 4, 107; 22. Tresina, P. S.; Mohan, V. R.; Trop. Subtrop. Agroecosyst. 2012, 15, 539; Mello, J. I. O.; Barbedo, C. J.; Salatino, A.; Figueiredo-Ribeiro, R. C. L.; Braz. Arch. Biol. Technol. 2010, 53, 889; Joshi, R.; Garg, B. D.; J. Sci. Res. 1981, 3, 11; Giral, F.; Mariel, R.; Cienc. Mex. 1959, 19, 27.

HCA revealed 93.8% similarity among the fatty acids found in the black and red seeds from E. velutina collected on the island of Fernando de Noronha and reds seeds from E. velutina collected in the state of Bahia (Figure 2). This similarity was supported by the PCA (Figure 3), in which the first two components explained 79% of the variation in the data (PC1 = 57% and PC2 = 22%). The graph shows the formation of a distinct group formed by the red and black seeds in the present study and the seeds from E. velutina collected in the state of Bahia.

Figure 2
Hierarchical cluster analysis of similarity in fatty acid profile among species of Erythrina
Figure 3
Principal component analysis of fatty acids in seeds from species of Erythrina

These results show that, despite differences in the chemical composition of the essential oils from the red and orange flowers of E. velutina, the chemical composition of the fatty acids in the black and red seeds of these plants did not differ. Therefore, despite the difference in the color of the seeds, the samples have the same chemotype.

CONCLUSION

This is the first study on the chemical composition of essential oils from the flowers of E. velutina. The oils from the orange and red flowers exhibit significant qualitative and quantitative differences in the chemical profile, which were confirmed by principal component analysis. Although phenylpropanoids are the predominant chemical class in both oils, (E)-caryophyllene is the major constituent in the orange flower oil, whereas (E)-asarone is the major constituent of the red flower oil.

Oleic acid, palmitic acid and behenic acid were the main fatty acids found in the red and black seeds of E. velutina. PCA and HCA confirmed a high degree of similarity in the fatty acid profile of the two chromatic variations of seeds.

ACKNOWLEDGMENTS

The authors are grateful to the Brazilian fostering agencies Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for awarding a grant (CAPES – Proc. # IBPG-0984-5.01/10), productivity scholarship (CNPq, # 312277/2013-0) and funding for this study (CNPq # 403162/203-0; FACEPE # APQ-1008-1.06/15; APQ-0476-1.06/14; APQ-08601.06/16).

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    http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB22965, accessed in October 2018.
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Publication Dates

  • Publication in this collection
    Jan 2019

History

  • Received
    11 July 2018
  • Accepted
    24 Oct 2018
  • Published
    07 Nov 2018
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