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Revista do Colégio Brasileiro de Cirurgiões

Print version ISSN 0100-6991

Rev. Col. Bras. Cir. vol.38 no.3 Rio de Janeiro May/June 2011

http://dx.doi.org/10.1590/S0100-69912011000300012 

TECNIC NOTE

 

Use en autologous plasma in abdominoplasty – previous note

 

 

Angélica Maria Schettino; Diogo Franco Vieira de Oliveira, TCBC-RJ; Talita Romero Franco, ECBC-RJ

IMaster's Degree Graduate, Plastic Surgery Service, HUCFF-UFRJ – Rio de Janeiro, Brazil
IIAssociate Professor, Plastic Surgery Service, HUCFF-UFRJ – Rio de Janeiro, Brazil
IIIProfessor, Plastic Surgery Service, HUCFF-UFRJ – Rio de Janeiro, Brazil

Correspondence to

 

 


ABSTRACT

Autologous plasma began to be studied in the 90's, mainly because its adhesive and angiogenic properties and the presence of growth factors of platelet origin. In fact, plasma can be isolated from autologous manner, from the patient's own blood and obtained in its two parts: a high concentration of platelets (platelet-rich plasma – PRP) and one with low concentration of platelets (platelet-poor plasma – PPP). The present study is in development at the Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro (UFRJ-HUCFF) and Marcilio Dias Naval Hospital (HNMD), both in Rio de Janeiro. The objective is to evaluate the properties of platelet-poor plasma, particularly its adhesive action, in patients referred for restorative abdominal dermolipectomy, to reduce postoperative collections, such as hematoma and seroma, two major complications in this type of surgery.

Key words: Reconstrutive surgical procedures. Wound healing. Tissue adhesives. Platelet-rich plasma.


 

 

INTRODUCTION

The platelet-rich plasma has important properties that help in healing and reduce postoperative swelling and fluid collection, hematoma and seroma. After activation with autologous thrombin, it displays adhesive power, and it can be termed platelet gel, in the case of platelet-rich plasma (PRP) or biological glue, in the case of the use of the less concentrated portion – platelet-poor plasma (PPP). Among its properties there are still the hemostatic and angiogenic ones, deriving from the large amount of platelet growth factors in the plasma1,2.

The research with autologous plasma is being carried out in patients who require restorative abdominoplasty at the Clementino Fraga Filho University Hospital and Marcilio Dias Naval Hospital, both in Rio de Janeiro, and it has been approved by the Ethics in Research Committee. The study thus accompanies the recent world studies2 in search of biological sealants, with the advantage of being an autologous blood component, which also causes, as it indicates, a decrease of the most frequent complications in the postoperative period of patients undergoing surgery with detachments and large flaps – the seroma. The plasma is presented as a good choice for industrial adhesives, which have similar properties of adhesion and hemostasis, but with higher costs and also the risk, albeit small, of viral transmission and allergic reactions.

 

METHODS

The plasma is isolated from blood collected from the patient during anesthesia. The collected blood is taken to the hematology laboratory and, according to an already established protocol3,4, subjected to centrifugation for a period of ten minutes at 400G. With this procedure, there is separation of the plasma, red blood cells and an intermediate layer, called the fog zone or buff coat, composed of leukocytes. The plasma is separated and submitted to another centrifugation, this time at 800G, also for ten minutes. The volume is then reduced to a third, separating a portion rich in platelets (one-third of the total) and one with a slightly lower concentration (two thirds of total volume). Both have platelets and thus growth factors.

The plasma is mixed with (also autologous) thrombin before being used. (Figure 1)

 

 

Thrombin is added to the plasma in order to obtain the gelation of plasma. The production of thrombin also follows the same obtaining protocol1, with inclusion of calcium, a compound that serves as thrombin activator.

To date, we analyzed data from 12 patients undergoing abdominoplasty using autologous plasma in its less platelet-concentrated portion – biological glue (Figure 2).

 

 

Initial results

There was a reduction in the time of continuous suction drain, normally used in this type of operation because of the large detachment. There was also reduction in the number of seromas and, therefore, in the number of aspiration punctures in the postoperative period after removal of drains. The longest follow-up of ambulatory patients is three months.

Comments

The research results are still preliminary and need further study, but show promise. There seems to be a reduction in postoperative collections, mainly seroma, reducing the need for punctures in the office and the time of permanence of continuous suction drains. All that at low cost, resulting only from the use of syringes, test tubes and the structure of the laboratory, with centrifuges and laminar flow, present in any hospital environment.

The research will continue in order to increase the number of patients undergoing the procedure with autologous plasma usage and the data will be analyzed in comparison with literature data and with a control group.

 

REFERENCES

1. Vendramin FS, Franco D, Schamall RF, Franco TR. Utilização do plasma rico em plaquetas (PRP) autólogo em enxertos cutâneos em coelhos. Rev soc bras cir plast 2010; 25(1):4-10.         [ Links ]

2. Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001; 107(1):229-37; discussion 238-9.         [ Links ]

3. Vendramin FS, Franco D, Nogueira CM, Pereira MS, Franco TR. Plasma rico em plaquetas e fatores de crescimento: técnica de preparo e utilização em cirurgia plástica. Rev Col Bras Cir 2006; 33(1):24-8.         [ Links ]

4. Vendramin FS. Plasma rico em plaquetas: estudo de obtenção e utilização nas cirurgias de enxerto de pele [tese].  Rio de Janeiro: Universidade Federal do Rio de Janeiro, Faculdade de Medicina, 2008.         [ Links ]

 

 

Correspondence to:
Diogo Franco
E-mail: contato@diogofranco.com

Received 23/12/2010
Accepted for publication 26/02/2011
Conflict of interest: none
Source of funding: none

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