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Pesquisa Veterinária Brasileira

Print version ISSN 0100-736XOn-line version ISSN 1678-5150

Pesq. Vet. Bras. vol.38 no.5 Rio de Janeiro May 2018

https://doi.org/10.1590/1678-5150-pvb-5116 

LIVESTOCK DISEASES

Genetic engineering of Theileria parva lactate dehydrogenase gene: a new anti-theilerial target

Irmak Icen-Taskin2  * 

Omer Munzuroglu3 

Hikmet Geckil4 

2 Department of Biology, Firat University, 23119 Elazig, Turkey.

3 Department of Bioengineering, Firat University, 23119 Elazig, Turkey.

4 Department of Molecular Biology and Genetics, Inonu University, 44280 Malatya, Turkey.


ABSTRACT:

Theileria parva is the causative agent of East Coast Fever (ECF), a tick borne disease, which results in major economic losses in cattle. Major problems in dealing with this illness are the high cost of drugs, development of resistance, and absence of effective vaccines. Thus, exploiting new targets for cost effective and higher therapeutic value drugs are imperative. Glycolysis is the main pathway for generation of ATP in T. parva, given its development inside erythrocytes. Thus, the enzymes of this pathway may prove potential targets for designing new-generation anti-theilerials. Lactate dehydrogenase of T. parva (TpLDH) has the highest activity of all glycolytic enzymes and thus we selected this enzyme as the potential therapeutic target. Our study is the first to report the isolation, removal of introns through directed mutagenesis, and cloning of TpLDH and showing that amino acid insertions or deletions most notably corresponded to a 5-amino acid sequence (Asn-91A, Glu-91B, Glu-91C, Trp-91D, Asn-91E) between Ser-91 ve Arg-92 of the enzyme. This region is also present in other apicomplexan such as Babesia bovis, a pathogen of cattle and Plasmodium falciparum, a human pathogen. Providing as the attachment site for the enzyme inhibitors and not being present in LDH of respective hosts, we propose this site as an attractive drug target. The work here is expected to lead new studies on detailed structural and kinetic aspects of apicomplexan LDHs and development of new inhibitors.

INDEX TERMS: Theileria parva; lactate dehydrogenase; anti-theilerial; East coast fever; parasite

Introduction

Theileria spp. are tick borne protozoan parasites that are members of the phylum Apicomplexa which includes many species of both medical (e.g., Plasmodium spp.; Toxoplasma) and veterinary (e.g. Babesia, Eimeria, Theileria) importance (Shaw 1997). T. parva is one of the two economically important species of the Theileria genus transmitted by Rhipicephalus ticks (Yamada et al. 2008) and causes an often fatal lympho-proliferative disease of cattle (Morrison 2009) known as East Coast Fever (ECF). The disease caused by the parasite affects a large proportion of domestic and wild animals. The parasite is highly pathogenic for cattle, leading to serious economic losses (Mukhebi et al. 1992).

As the disease is tick-borne, it is hypothesized that breaking any of the links between the chain of host and pathogen would help prevent ECF. An important problem is the high cost of the acaricides used for tick control (Walker 2007) and the rapid resistance development (Benavides et al. 2000, Miller et al. 2002). Moreover, to date, there is no safe and effective vaccine against Theileria spp. (Elisa et al. 2015, Osman & Al-Gaabary 2007, Walker 2007). Buparvaquone, an antiprotozoal drug, is widely shown to be an effective antitheilerial (Dolan et al. 1992, McHardy et al. 1985). Although antitheilerials are used in the treatment of the disease (Dolan et al. 1992, McHardy et al. 1985), their efficacy is limited (Singh et al. 1993). Also, development of resistance to antitheilerials is a major problem (Marsolier et al. 2015, Mhadhbi et al. 2010, 2015, Sharifiyazdi et al. 2012). Thus, there is an urgent need for a safe, effective, and inexpensive antitheilerial.

Potential weak points of the energy metabolism may provide a chemotherapeutic target for the design of a new antitheilerial. However, almost the impossibility of removing all T. parva schizonts without any contamination by host materials (Hung & Parkinson 2011), cost, and length of isolation have delayed the studies on the enzymes of energy metabolism in schizonts (Kiama et al. 1999). On the other hand, previous studies showed that glycolytic enzymes had high activity in T. parva schizonts and this being highest for the lactate dehydrogenase (Kiama et al. 1999). This is the type of energetic metabolism utilizing glycolysis as the main route for energy production (Kiama et al. 1999) is similar to that of Plasmodium in the erythrocytic stage (Sherman 1979). Given this, LDH enzyme of the Theileria parva was selected as the target for structure-based drug design studies, as was the case for others apicomplexan (Cameron et al. 2004, Nural et al. 2016).

Building on the present day knowledge of structural differences between the host and the parasite LDH, we have isolated and cloned Theileria parva lactate dehydrogenase (TpLDH) gene to study its potential as the new target for the development of selective inhibitors.

Materials and Methods

Bacterial cloning and growth. Unless otherwise stated, all general methods were followed according to well-established standard protocols (Sambrook & Russell 2006). Genomic DNA including TpLDH gene was obtained from ILRI (International Livestock Research Institute, Nairobi, Kenya). The host bacterial strain was Escherichia coli JM103 (endA1 glnV44 sbcBC rpsL thi-1 Δ (lac-proAB) F’[traD36 proAB+ lacIq lacZΔM15). TpLDH gene was cloned using the pGEM-T easy vector sytem I (Promega, USA). Growth medium was Luria-Bertani Broth (LB). When required, 0.1% (v/v) of a filter sterilized solution of 100mg/ml-1 ampicillin was added after autoclaving the medium. The selection of presumptive transformants was done on LB-agar plates containing 20mg/ml IPTG, 20mg/ml X-Gal and 100μg/ml ampicillin.

Amplification of TpLDH. The initial sequence of TpLDH gene was retrieved from NCBI with the accession number of XM_761610.1. The sequence was utilized to design two particular oligonucleotide primers for the amplification of the LDH gene from genomic DNA of T. parva. The forward primer was TpLDHF and reverse primer was TpLDHR:

5’-ATGTCTGGAATTAGGAGGAAGTTGATTAG-3’

5’-TTATTTGATGAGTGATTCTAGTCGTCTG-3’

The PCR was performed as follow; the mixture of PCR contained 5μl of Taq DNA Polymerase buffer (10X), 3μl MgCl2 (25mM), 5μl of a stock of dNTPs (2mM), 2.5μl forward oligonucleotide primer (20pmol), 2.5μl reverse oligonucleotide primer (20pmol), 0.5μl T.parva genomic DNA (50ng), 0.5μl Taq DNA Polymerase (5 units), and H2O to a final volume of 50μl. A full thermal cycle was adjusted to 94°C for 1.5 min for DNA denaturation, 55°C for 2 min for annealing, and 72°C for 2 min for the extension for a total of 30 cycles. The confirmation of the amplified DNA was done on a 1% agarose gel from which DNA band was extracted using QIA Quick Gel Extraction Kit (QIAGEN).

Ligation and transformation. Gel purified PCR products were inserted into pGEM-T easy vector system (Promega) and cloned into E. coli JM103 using standard cloning protocols. This vector contains multiple cloning sites (MCS) containing a range of restriction enzyme recognition sites, the lacZ gene for blue white selection and an ampicillin resistance gene for antibiotic selection. The mixture for ligation of PCR products to pGEM-T easy subcloning vector contained 5μl 2X ligase buffer, 1μl pGEM-T easy vector, 100ng DNA (insert), 1μl T4 ligase and sterile dH2O to complete the volume to 10μl. Ligation was carried out at 4°C for 16 h. The recombinant plasmid containing the TpLDH gene transformed to the competent bacterial cells prepared by the calcium chloride method.

Removing introns by site-directed mutagenesis. Site-directed mutagenesis was performed to remove two introns present in the TpLDH gene (Horton et al. 2013). Briefly, TpLDH gene was amplified as 3 (A, B and C) gene fragments which were complementary to each other at the end:

TpLDHF 5’-ATGTCTGGAATTAGGAGGAAGTTGATTAG-3’ and TpLDHEx1R 5’-CAGCGCCAATATTGGGGACAATATC-3’,

TpLDHEx2F 5’- GATATTGTCCCCAATATTGGCGCTG-3’ and TpLDHEx2R 5’- AGCCTTCGCCAAACCGGCGGTAAC-3’,

TpLDHEx3F 5’- GTTACCGCCGGTTTGGCGAAGGCT-3 and TpLDHR 5’- TTATTTGATGAGTGATTCTAGTCGTCTG-3’

Above primers were used to produce A, B and C fragments, respectively. Using Go taq DNA Polymerase, the fragments were amplified by PCR using the appropriate primer set with 30 cycles, each with denaturation, annealing and extension at 94°C for 1 min, 55°C for 2 min and 72°C for 2 min, respectively. The three overlapping fragments were then joined by overlap extension reaction.

DNA sequencing and bioinformatics tools for molecular modeling. Cloned TpLDH gene was firstly checked through colony PCR using the TpLDHF and TpLDHR primers to confirm the correct DNA locus. Then plasmid DNA was isolated using QIA Miniprep Kit (QIAGEN) according to the manufacturer instructions and sent to Iontek (Istanbul) for sequencing.

A range of Web-based tools were used for analysis of DNA and protein sequences. Lactate dehydrogenase sequences of apicomplexan parasites and the host B. taurus were obtained from National Centre for Biotechnology Information (NCBI). The primers were designed based on the NCBI reference sequence (NC_007344.1) for the provisional TpLDH from the whole genome sequence of T. parva. The LDH sequences of T.annulata (accession no: XM_948495) and B. taurus (accession no: NM_174099.2) were used for comparison. Both strands of TpLDH were sequenced and the alignment results were compared using Lipper Centre for Computational Genetics of Harvard University. ClustalW2 tool was used to align the sequences at the nucleotide and amino acid level (Chenna et al. 2003). The positions of exons and introns were determined via the GSDS application (Guo et al. 2007). Homology modeling of BtLDH-A and TpLDH was conducted by using Phyre2 web (Kelley et al. 2015).

Results

Amplification, cloning and DNA sequencing of the Theileria parva LDH gene

Amplification and cloning of a predicted 1353bp band from T. parva genomic DNA was successfully carried out by using TpLDHF and TpLDHR oligonucleotides. After extraction, the PCR products were analyzed on a 1% (w/v) agarose gel electrophoresis (Fig.1), and bands with expected size (1353bp) were subcloned into pGEM®Teasy vector which was transformed into E.coli JM103. Sequences analysis showed that 1353 base pair length TpLDH gene contained 2 introns, 3 exons.

Fig.1. Agarose gel electrophoresis of PCR products amplified TpLDH gene from genomic DNA of Theileria parva. M = Marker, 1 = TpLDH. 

Removing of introns from TpLDH by using site-directed mutagenesis

The sequences analysis indicated that TpLDH gene is 1353 base pair in length and contains 3 open reading frames (ORF) of 121bp, 128bp, and 717bp, named as A, B, and C fragments respectively. The fragments were separated by two introns which were removed by site-directed mutagenesis (Fig.2A). Three sets of oligonucleotides primers were used to resolve the overlapping exons. These results showed that site-directed mutagenesis can effectively be used to remove introns in the case of difficulty arising from obtaining cDNA. The fragments (i.e. A, B, C) were then combined with PCR amplification through their complementary end sequences, resulting the full length 966bp coding gene starting with ATG and ending with TAA (stop) codon (Fig.2B).

Fig.2. Agarose gel electrophoresis of PCR products amplified to produce (A) overlapping exon fragments from TpLDH gene of Theileria parva: M = Marker, 1 = A fragment (Exon 1), 2 = B fragment (Exon 2), 3 = C fragment (Exon 3). (B) Overlapping A, B and C fragment to obtain TpLDH gene without introns: M = Marker, 1 and 2 = TpLDH without intron. 

Multiple amino acid sequence alignment

Isolated and cloned nucleotide sequence of TpLDH gene was aligned and compared both with Theileria annulata lactate dehydrogenase (TaLDH) and the host Bos taurus lactate dehydrogenase (BtLDH) genes. Results showed that the mutations on TpLDH corresponded to the same position on BtLDH which manifested themselves in similar amino acid insertions or deletions on the LDH enzyme. Most notable difference between TpLDH and BtLDH was a penta peptide (Asn-91A, Glu-91B, Glu-91C, Trp-91D, Asn-91E) insertion on TpLDH between positions serine 91 and arginine 92, making the active site loop of the enzyme. This five amino acid insertion is also present in TaLDH sequences (Fig.3).

Fig.3. Multiple alignment result of lactate dehydrogenase of Theileria parva (TpLDH), T. annulata (TaLDH, NCBI Reference Sequence: XM_948495.1) and Bos taurus (BtLDH, NCBI Reference Sequence: NM_174099.2) = Penta peptide insertion is shown in red frame. 

Homology modeling of Theileria parva and Bos taurus LDHs

The amino acid sequence acquired after the exclude of the two introns from the gene utilized to demonstrate the 3D structure of TpLDH by Phyre2 web portal (Fig.4A). BtLDH amino acid sequence (AAI46211.1) data from GenBank was also used for comparison (Fig.4B). The substrate specificity loop (residues 92-96) has a five residue insert which is absent in BtLDHs (Fig.4C).

Fig.4. (A) 3D structure of TpLDH and (B) BtLDH in ribbon style. (C) Superposition of the predicted structure TpLDH with BtLDH; five amino acid insertion in the substrate specificity loop of TpLDH (arrow). 

Discussion

By overcoming the isolation problem of Theileria parva (Goddeeris et al. 1991) and consequently after the completion of the genome project (Gardner et al. 2005), significant data were obtained about the metabolism of this parasite. While the parasite encodes all the enzymes required for the glycolysis and tricarboxylic acid (TCA) cycle (Gardner et al. 2005), the data obtained suggest that TCA cycle in T. parva is not a conventional one (Lau 2009). The same is true for Plasmodium (Foth et al. 2005, Ralph 2005) and other apicomplexan (Lau 2009, Pino et al. 2007). This raises a fundamental question regarding the functionality of TCA cycle in T. parva (Gardner et al. 2005). It is assumed that glycolysis is the sole pathway for energy generation in T. parva and plays a formidable role in its life cycle. Thus, targeting the enzymes of this pathway (Loo et al. 2010, Schaap et al. 2004) as well as lactate dehydrogenase as anti-theilerials may prove advantageous.

The crystal structure of Plasmodium falciparum LDH (PfLDH) reveals its unusual properties. Compared to its mammalian counterparts it has a significant shift in the position of the NADH cofactor (Dunn et al. 1996). PfLDH has also unique structural differences in comparison to host LDHs. The antigenic loop (residues 205-221) in PfLDH adopts a different conformation from vertebrate LDHs (Dunn et al. 1996). It is assumed that this loop is responsible for the antigenic discrimination. Moreover, the substrate specificity loop (residues 97-112) has a penta peptide insertion (Bzik et al. 1993) that forms a separate cavity in the surface adjacent to the catalytic region which is absent in mammalian LDHs (Dunn et al. 1996). These structural differences could be exploited for selective targeting of the parasite enzyme (Alam et al. 2014). Based on the previous investigations of Plasmodium species (Cameron et al. 2004, Dunn et al. 1996), we aimed to inquire the possibility of designing new anti-theilerial drugs from the data on TpLDH in this study. To our knowledge, this is the first study to clone and characterize TpLDH in terms of its potential as a new drug target against East Coast Fever. The structural analysis showed that compared to the host enzyme, the LDH of T. parva had a profound difference in the substrate binding site, where a penta peptide is present. This five amino acid insertion, which is absent from host enzyme, may provide an effective drug target. With varying sequences, other apicomplexan were also shown to have such penta peptides on the active region (Dunn et al. 1996, Kavanagh et al. 2004, Schaap et al. 2004) which constitutes an adsorption region for inhibitors (Dunn et al. 1996). In a study conducted by Erdemir et al., it has also shown that there is same penta peptide insertion in lactate dehydrogenase of Theileria annulata (Erdemir et al. 2012). In line with these, TpLDH enzyme could be utilized as a specific target for inhibitors without harming the vertebrate host.

Conclusions

This study appears to be the first to report the isolation, removal of introns through directed mutagenesis, and cloning of TpLDH and showing that amino acid insertions or deletions.

As an important finding, a penta-peptide (Asn-91A, Glu-91B, Glu-91C, Trp-91D, Asn-91E) between Ser-91 ve Arg-92 of the enzyme was defined in TpLDH through comparing to the homology model of TpLDH and its counterpart host BtLDH.

It was determined that the insertion region, which is present on TpLDH, do not exist in host BtLDH. Hence, this insertion site can be used as the binding sites for specific enzyme inhibitors to be developed

Acknowledgements

We thank Venhar Celik for her contribution to this study. We also thank Dr. Roger Pelle (International Livestock Research Institute, Nairobi, Kenya) for providing us the genomic DNA of the Theileria parva. This study has been supported by Firat University Scientific Research Projects Unit (FUBAP) with FF.11.16 project number

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Received: April 14, 2017; Accepted: May 08, 2017

*Corresponding author: irmak.icen@hotmail.com

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