Overcoming crossing barriers between cassava, Manihot esculenta Crantz and a wild relative, M. pohlii Warwa

Nassar, Nagib M.A.; Carvalho, Claudio G.; Vieira, Clibas

doi:10.1590/S0100-84551996000400015

Abstract

The use of mentor pollen has enabled successful hybridization between cassava, Manihot esculenta Crantz, and the wild species M. pohlii Warwa. Killed pollen of a cross compatible type produced by freeze-thawing was mixed with incompatible pollen and the mixes were dusted on stigmas. This treatment resulted in production of seed in 4.9% of the total pollinations, compared to 0% in the case of untreated pollinations. The use of a bridge species, M. neusana Nassar, through the hybrid M. pohlii and M. neusana also proved successful in overcoming interspecific barriers between cassava and M. pohlii.

overcoming crossing barriers; cassava; Manihot esculenta Crantz; wild relative; M. pohlii Warwa

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Overcoming crossing barriers between cassava, Manihot esculenta Crantz and a wild relative, M. pohlii Warwa

Nagib M.A. Nassar^I; Claudio G. Carvalho^I; Clibas Vieira^II

^IDepartamento de Agronomia, Universidade de Brasilia, Caixa Postal 04477, 70919 Brasília, DF, Brasil. Send correspondence to N.M.A.N.

^IIDepartamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil

ABSTRACT

The use of mentor pollen has enabled successful hybridization between cassava, Manihot esculenta Crantz, and the wild species M. pohlii Warwa. Killed pollen of a cross compatible type produced by freeze-thawing was mixed with incompatible pollen and the mixes were dusted on stigmas. This treatment resulted in production of seed in 4.9% of the total pollinations, compared to 0% in the case of untreated pollinations. The use of a bridge species, M. neusana Nassar, through the hybrid M. pohlii and M. neusana also proved successful in overcoming interspecific barriers between cassava and M. pohlii.

Keywords: overcoming crossing barriers; cassava; Manihot esculenta Crantz; wild relative; M. pohlii Warwa.

Brewer, J.L. and Henstra, S. (1974). Pollen of Pyrethrum. Fine structure recognition. Euphytica 23: 657-663.
Dayton, D.F. (1974). Overcoming self incompatibility in apple with killed compatible pollen. J. Amer. Soc. Hort. Sci. 99: 190-192.
Dionne, L.A. (1963). Studies on the use of Solanum acaule as a bridge between Solanum tuberosum and species in the series Bulbocastana, cardiophylla and Pinnatisecta. Euphytica 12: 263-269.
Howlett, B.J., Knox, R.B., Paxton, J.H. and Heslop-Harrison, J. (1975). Pollen-wall proteins: Physiochemical characterization and role in self incompatibility in Cosmos bipinnatus. Proc. R. Soc. Br. 188: 167-182.
Knox, R.B., Willing, R.R. and Pryor, L.D. (1972). Interspecific hybridization in Populus using recognition pollen. Silvae Genetica 21: 3-4.
Nassar, N.M.A. (1978). Wild Manihot species of Central Brazil for cassava breeding. Can. J. Plant Sci. 58: 257-261.
Nassar, N.M.A. (1985). Manihot neusana Nassar: A new species native to Paraná, Brazil. Can. J. Plant Sci. 65: 1097-1100.
Nassar, N.M.A. (1986). Genetic variation of wild Manihot species native to Brazil and its potential for cassava improvement. Field Crop Res. 13: 177-184.
Nassar, N.M.A., da Silva, J.R. and Clibas Vieira, C. (1986). Hidridização interespecífica entre mandioca e espécies silvestres de Manihot. Ciênc. Cult. 33: 1050-1055.
Nassar, N.M.A. (1989). Broadening the genetic base of cassava, Manihot esculenta Crantz by interspecific hybridization. Can. J. Plant Sci. 69: 1071-1073.
Sastri, D.C. and Shivanna, K.R. (1976). Attempts to overcome interspecific incompatibility in Sesanum by using recognition pollen. Ann. Bot. 40: 891-893.
Stettler, R.F. (1968). Irradiated mentor pollen: Its use in remote hybridization of the cottonwood. Nature 219: 746-747.
Williams, R.R. and Church, R.M. (1975). The effect of killed compatible pollen on self compatibility in apple. J. Hort. Sci. 30: 457-461.

Publication Dates

Publication in this collection
26 Oct 2006
Date of issue
1996

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Brewer, J.L. and Henstra, S. (1974). Pollen of Pyrethrum. Fine structure recognition. Euphytica 23: 657-663.

[2] Dayton, D.F. (1974). Overcoming self incompatibility in apple with killed compatible pollen. J. Amer. Soc. Hort. Sci. 99: 190-192.

[3] Dionne, L.A. (1963). Studies on the use of Solanum acaule as a bridge between Solanum tuberosum and species in the series Bulbocastana, cardiophylla and Pinnatisecta. Euphytica 12: 263-269.

[4] Howlett, B.J., Knox, R.B., Paxton, J.H. and Heslop-Harrison, J. (1975). Pollen-wall proteins: Physiochemical characterization and role in self incompatibility in Cosmos bipinnatus. Proc. R. Soc. Br. 188: 167-182.

[5] Knox, R.B., Willing, R.R. and Pryor, L.D. (1972). Interspecific hybridization in Populus using recognition pollen. Silvae Genetica 21: 3-4.

[6] Nassar, N.M.A. (1978). Wild Manihot species of Central Brazil for cassava breeding. Can. J. Plant Sci. 58: 257-261.

[7] Nassar, N.M.A. (1985). Manihot neusana Nassar: A new species native to Paraná, Brazil. Can. J. Plant Sci. 65: 1097-1100.

[8] Nassar, N.M.A. (1986). Genetic variation of wild Manihot species native to Brazil and its potential for cassava improvement. Field Crop Res. 13: 177-184.

[9] Nassar, N.M.A., da Silva, J.R. and Clibas Vieira, C. (1986). Hidridização interespecífica entre mandioca e espécies silvestres de Manihot. Ciênc. Cult. 33: 1050-1055.

[10] Nassar, N.M.A. (1989). Broadening the genetic base of cassava, Manihot esculenta Crantz by interspecific hybridization. Can. J. Plant Sci. 69: 1071-1073.

[11] Sastri, D.C. and Shivanna, K.R. (1976). Attempts to overcome interspecific incompatibility in Sesanum by using recognition pollen. Ann. Bot. 40: 891-893.

[12] Stettler, R.F. (1968). Irradiated mentor pollen: Its use in remote hybridization of the cottonwood. Nature 219: 746-747.

[13] Williams, R.R. and Church, R.M. (1975). The effect of killed compatible pollen on self compatibility in apple. J. Hort. Sci. 30: 457-461.