Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues

Braz J Med Biol Res, August 1998, Volume 31(8) 1059-1067

Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues

V.I. Lushchak^1,2, T.V. Bahnjukova² and K.B. Storey³

¹Department of Medical Biology and Genetics, Ivano-Frankivsk State Medical Academy, Ivano-Frankivsk, Ukraine

²Karadag Branch of Southern Sea Biology Institute, Kurortne, Feodosia, Crimea, Ukraine

³Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, Ontario, Canada

Abstract

Introduction

Material and Methods

Results

Discussion

References

Acknowledgments

^{Correspondence and Footnotes}

The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK) by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three tissues were partitioned between soluble and particulate-bound forms. In several cases, the percentage of bound enzymes was reduced during hypoxia; bound aldolase in brain was reduced from 36.4 to 30.3% whereas glucose-6-phosphate dehydrogenase fell from 55.7 to 28.7% bound. In muscle PFK was reduced from 57.4 to 41.7% bound. Oppositely, the proportion of bound aldolase and triosephosphate isomerase increased in hypoxic muscle. Phosphoglucomutase did not appear to occur in a bound form in liver and bound phosphoglucomutase disappeared in muscle during hypoxia exposure. Anoxia exposure also led to the disappearance of bound fructose-1,6-bisphosphatase in liver, whereas a bound fraction of this enzyme appeared in white muscle of anoxic animals. The possible function of reversible binding of glycolytic enzymes to subcellular structures as a regulatory mechanism of carbohydrate metabolism is discussed.

Key words: fish, sea scorpion, Scorpaena porcus, hypoxia, metabolites, glycolytic enzyme binding

Glycolytic and associated enzymes can bind reversibly to various cellular structures such as glycogen particles, F-actin, microfilaments (1-5) and membranes (6-8). These interactions can change some of their functional properties. For example, bound lactate dehydrogenase (LDH) is not inhibited by high concentrations of pyruvate (7,9,10), and is more stable to trypsinolysis than the free enzyme (10). Catalytic properties can also be modified by enzyme interaction with structural elements (1,2,7,10,11). The distribution of enzymes between free and bound forms may, in turn, be modulated by changes in metabolite and ion levels (6,12,13) or by reversible protein phosphorylation (3). Various studies on muscle have further assessed the possible existence of a glycolytic complex that could facilitate pathway flux by grouping together all of the glycolytic enzymes in a binding association with subcellular structural elements. The complex has never been conclusively documented experimentally, not surprising considering the weak nature of the binding contacts between enzymes and structural elements (14). Different attempts have been made to stabilize such a complex such as the addition of crowding agents to the extraction medium (e.g. polyethylene glycol) that should increase the stability of enzyme-enzyme interactions during their isolation (14,15).

Although many questions about the functional consequences of glycolytic enzyme binding to the subcellular particulate fraction are not yet resolved, several researchers, using different techniques, have documented the redistribution of various enzymes between free and bound fractions as a function of changes in the physiological state of organisms. For instance, the percentage of glycolytic enzymes bound to subcellular particulate matter was altered in response to electrical stimulation of bovine muscle (16), anoxia in marine gastropods (17), or anoxia, hypoxia or exercise in fish (11,18,19).

Exposure to hypoxia elicits multiple biochemical and physiological adjustments in fish tissues (20,21). Immediate responses include an activation of anaerobic glycolysis and the breakdown of creatine phosphate which increase ATP production at a time when mitochondrial oxidative phosphorylation is limited by oxygen availability. Over the longer term physiological and biochemical adjustments occur which improve the ability to extract oxygen from hypoxic water or reduce the animal's dependence on oxygen, if hypoxia is severe. The latter adjustments can include a switch to specific fermentative pathways that have advantages over anaerobic glycolysis alone or the initiation of metabolic rate depression so that cellular energy demands are sharply suppressed and coordinated with the reduced rates of anaerobic ATP production (21).

It is evident that the glycolytic pathway is central to hypoxia survival in fish. Although the response of the pathway under hypoxia has been extensively investigated (22-28), the mechanisms which provide the metabolic adaptation are still not fully understood. Certainly, these processes are highly coordinated and many mechanisms such as allosteric effects of metabolites, reversible protein phosphorylation, and enzyme binding interactions with cellular structural elements may participate in the control of the overall response to hypoxia (29). The last mechanism, enzyme binding, remains poorly studied. The present study was designed to further investigate the effects of hypoxia exposure on the distribution of enzymes between free and particulate-bound fractions in tissues of teleost fish and to assess the effects that such changes may have on carbohydrate metabolism under hypoxia.

Experimental animals

Sea scorpions (Scorpaena porcus L.) weighing 120-400 g were caught in June-July 1995 at Karadag Bay, Black Sea (Crimea, Ukraine) and were held in tanks provided with flowing sea water and the native dark/light cycle at a temperature of 20-21^oC. Fish were not fed and were used the next day.

Biochemicals and coupling enzymes

All chemicals were obtained from Boehringer Mannheim, Montreal, PQ; Sigma Chemical Co., St. Louis, MO, and Reachim (USSR).

Exposure to hypoxia

A continuous-flow system was used for the experiments. Fish were placed in a 10-liter experimental chamber equipped with a water pump. This chamber was placed in a larger aquarium that was stabilized at 20.6 ± 1.0^oC. The temperature and oxygen levels were monitored with a two-channel oxymeter purchased from Sigma (Charkiv, Ukraine). Electrodes were calibrated to zero oxygen with a freshly prepared 2% sodium persulfate solution. Calibration to 100% was done with air.

Eight fish were carefully transferred from the tanks into the chamber at about 6:00-7:00 a.m. and held there for 1 h at 100% oxygen saturation. After this, four fish were sampled as controls and then the chamber was closed. The level of oxygen was allowed to gradually decrease over 90-120 min to 15% saturation as the result of its consumption by the fish. When the desired oxygen concentration was reached, this level was stabilized by bubbling air through the chamber and a constant 15% saturation was maintained over the subsequent 90 min.

Tissue sampling

Fish from the chamber were placed into a bucket with water from the chamber and sacrificed within 20 min. No essential differences in enzyme parameters were found between the first and the last animal in a group. The procedure from removal of the fish from the bucket through killing by spinal dissection, removal of tissue samples, and preparation of tissue homogenate took about 2 min. Whole brain and portions of the liver and white muscle were used for analysis. White muscle samples were removed from the body below the first spinal fin.

Metabolite extraction

For the analysis of glycogen, glucose and lactate, tissue extraction was performed in a "Potter-Elvehjam" homogenizer in 10-50 volumes of 60% ethanol, containing 8% perchloric acid (23). This procedure separates glycogen in the sediment after centrifugation whereas glucose and lactate remain in the supernatant (23). Glycogen in the precipitate and glucose in the supernatant were determined by the anthrone method whereas lactate was quantified enzymatically (30).

Enzyme studies

Excised fresh tissues were quickly washed in ice-cold 0.9% NaCl, blotted on filter paper, weighed and homogenized in a "Potter-Elvehjam" homogenizer in 10 volumes of homogenization solution of the following composition: 0.25 M sucrose, 10 mM dithiothreitol (DTT), 20 mM NaF, 1 mM EDTA, 20 mM imidazole-HCl, pH 7.0 at 20^oC, and a few crystals of the protease inhibitor phenylmethylsulfonyl fluoride. The homogenate was centrifuged for 20 min at 45,000 g at 4^oC and the supernatant was then removed and stored on ice. Enzyme activity in this supernatant represents the free enzyme fraction. The pellet was rehomogenized in 5 volumes (relative to initial tissue weight) of the following solution containing high salt to release bound enzymes: 0.25 M sucrose, 10 mM DTT, 20 mM NaF, 1 mM EDTA, 20 mM imidazole-HCl, pH 7.0 at 20^oC, and 500 mM KCl. After centrifugation, the supernatant was removed and the pellet was then extracted a second time and centrifuged and the second supernatant was combined with the first. Enzyme activity in this fraction represents the bound enzyme fraction. Maximal enzyme activities were quantified in both free and bound fractions and the percentage of activity in the bound fraction was calculated using the following formula: bound/(bound + free) x 100. Recovery of total enzyme activity was checked in all cases by comparing the sum of enzyme activities in free and bound fractions with enzyme activities measured in tissue homogenates; recovery was close to 100% in most case, ranging from 80-120%.

Enzyme activities were assayed at 25^oC using a KFK-324 spectrophotometer (LOMO, Leningrad, USSR) by monitoring the change in absorbance of NADH or NADPH at 340 nm. The activity of each enzyme was determined in four independently prepared supernatants of extracts from tissues of four individual fish. All reaction mixtures contained 50 mM imidazole-HCl, pH 7.0 at 25^oC, and the final volume was 1.0 ml including added extract. Blanks were run in all cases that omitted the most specific substrate (indicated by an asterisk in the assays below). In initial tests, reactions were started by addition of the most specific substrate, but if no blank was found, further assays were started by the addition of enzyme extract. Optimal assay conditions were based on those described earlier (31) and adapted for our experiments.

Enzymes were assayed under the following conditions: hexokinase (HK; EC 2.7.1.1): 10 mM glucose*, 0.2 mM NADP, 2 mM ATP, 5 mM MgCl₂, 0.5 unit (U) glucose-6-phosphate dehydrogenase (G6PDH), and 30-50 µl supernatant. Phosphoglucoisomerase (PGI; EC 5.3.1.9): 2.5 mM fructose-6-phosphate*, 0.2 mM NADP, 5 mM MgCl₂, 0.5 U G6PDH, and 5 µl supernatant. Phosphofructokinase (PFK; EC 2.7.1.11): 5 mM fructose-6-phosphate*, 5 mM ATP, 0.15 mM NADH, 10 mM MgCl₂, 50 mM KCl, 0.5 U aldolase, 0.5 U triosephosphate isomerase, 2 U glycerol-3-phosphate dehydrogenase, and 30-50 µl supernatant. Aldolase (Ald; EC 4.1.2.13): 0.5 mM fructose-1,6-bisphosphate*, 0.15 mM NADH, 0.5 U triosephosphate isomerase, 2 U glycerol-3-phosphate dehydrogenase, and 5-10 µl supernatant. Triosephosphate isomerase (TPI; EC 5.3.1.1): 4 mM glyceraldehyde-3-phosphate*, 0.15 mM NADH, 0.5 U glycerol-3-phosphate dehydrogenase, and 30-50 µl supernatant. Pyruvate kinase (PK; EC 2.7.1.40): 10 mM phosphoenolpyruvate*, 2.5 mM ADP, 50 mM KCl, 5 mM MgCl₂, 0.15 mM NADH, 0.5 U lactate dehydrogenase, and 5-10 µl supernatant. Lactate dehydrogenase (LDH; EC 1.1.1.27): 1 mM pyruvate*, 0.15 mM NADH, 1 mM EDTA, and 5-20 µl supernatant. Glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49): 2.0 mM glucose-6-phosphate*, 5 mM MgCl₂, 0.2 mM NADP, and 10-50 µl supernatant. Phosphoglucomutase (PGM; EC 2.7.5.1): 15 mM glucose-1-phosphate*, 0.20 mM NADP, 5.0 mM MgCl₂, 0.5 U G6PDH, and 10-30 µl supernatant. Fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11): 0.1 mM fructose-1,6-bisphosphate*, 0.2 mM NADP, 5 mM MgCl₂, 0.5 U G6PDH, 0.5 U phosphoglucoisomerase, and 10-50 µl supernatant.

One unit of enzyme activity was defined as the amount of enzyme which converts 1 µmol of substrate per minute at 25^oC (IU). All values are reported as mean ± SEM. Differences between the means were estimated by the two-tailed Student t-test.

Table 1 shows the effect of hypoxia on the levels of glycogen, glucose and lactate in three tissues of the sea scorpion: brain, liver and white muscle. Glycogen levels were highest in the liver and lowest in the brain. Exposure of fish to water with 15% oxygen saturation for 90 min did not significantly change the glycogen content of any tissue, although a definite trend to reduced mean glycogen levels after hypoxia was observed in all instances. Effects of hypoxia exposure on glucose levels were different in each tissue. In the brain, glucose was unaltered after hypoxia exposure whereas in hypoxic liver glucose decreased by 33%. By contrast, hypoxia led to a 6.9-fold increase in the glucose content of white skeletal muscle. The concentration of lactate in sea scorpion tissues was very low in all cases and was not changed after hypoxia exposure. The low lactate levels were consistent with a low activity level (and a possible metabolic depression) of the fish being observed during experimental hypoxia; after 30-40 min of hypoxia exposure the fish had lost their righting response and did not struggle when removed from the water. Since enzymes are responsible for the regulation of metabolic rate in organisms, we studied the effect of hypoxia on the activity of some glycolytic and other associated enzymes.

Hypoxia exposure altered both the maximal activities of some enzymes and the distribution of enzymes between free and bound forms in sea scorpion tissues. In the brain, the maximal activities of three enzymes were significantly elevated during hypoxia: HK by 23%, PGI by 47% and PFK by 56% (Table 2). However, in the liver only one hypoxia-induced change in enzyme activity was observed, i.e., a 17% decrease in PFK (Table 3). In white muscle hypoxia exposure resulted in a 27% decrease in PGI activity but a 64% increase in LDH (Table 4).

A high variation in the percentages of enzymes bound to subcellular particulate matter was found in sea scorpion tissues. In the liver, for example, about 60-65% of TPI was bound, whereas only 0-5% of FBPase was found in this state (Table 3). None of the PGM in liver was associated with the particulate fraction and bound FBPase in liver and PGM in muscle could not be detected in hypoxic tissues. Selected enzymes in each tissue showed significant changes in the percentage of activity in the bound fraction as the result of hypoxia exposure. In the brain, the percentage of bound Ald decreased and bound G6PDH fell by nearly half (Table 2). In hypoxic liver the percentage of bound HK increased by 2.7-fold, whereas all FBPase activity disappeared from the bound fraction (Table 3). In white muscle hypoxia exposure led to a reduced percentage of bound PFK and the disappearance of bound FBPase, whereas the percentages of bound Ald and TPI increased (Table 4).

Although alien to the physiology of man, a facility for metabolic depression occurs widely in nature. Metabolic depression is a key factor in facultative anaerobiosis, anhydrobiosis, hibernation, estivation, torpor and diapause. It allows organisms to economize on fuel and energy reserves in the face of environmental stress of unpredictable duration (29,32). The theory of metabolic depression applies well to fish in hypoxic or anoxic states. A drop in the contribution of oxidative phosphorylation to energy production and an enhancement of the anaerobic contribution are well documented but are often accompanied by an overall suppression of net ATP turnover that helps to conserve fuel use when inefficient fermentative pathways are the primary source of ATP (for reviews see 20,21,29). Therefore, the role of the glycolytic pathway in these states is critical.

The trend towards reduced glycogen levels in all three tissues during hypoxia as well as significant changes in glucose in liver and muscle were consistent with altered carbohydrate metabolism in hypoxic tissues (Table 1). In particular, glucose concentration rose 6.9-fold in muscle. Although lactate did not accumulate, the animals were clearly energy stressed, as shown by their loss of equilibrium and lack of muscle tone. Tissue specific changes in the levels of these and other glycolytic metabolites during hypoxia/anoxia exposure support the central role of glycolysis in metabolic ATP production at low oxygen levels in various other fish species including the Crucian carp Carassius carassius (22), European eel Anguilla anguilla (28), flounder Platichthys flesus (23,24) and red mullet Mullus barbatus (25). For instance, in red mullet the level of metabolites in the brain was not changed by 90-min exposure to water with 50% oxygen saturation, but in the liver glycogen and glucose concentrations were decreased about 3-fold (25).

The variation in maximal enzyme activities in the three tissues (Tables 2-4) correlates well with tissue function. For example, the high activities of most glycolytic enzymes in white muscle is associated with their use in supporting burst swimming for food capture, whereas activities of enzymes involved in biosynthetic processes, such as G6PDH and FBPase, were either undetectable or low in muscle. On the contrary, the liver showed substantial activities of both of these enzymes but lower overall activities of glycolytic enzymes.

It should be noted that the maximal activities of glycolytic enzymes in sea scorpion tissues were generally rather low compared with other animals and fish. This fact may be related to the low physical activity of this species. Sea scorpions spend much of their time motionless with infrequent bursts of activity for prey capture. It is possible that for such short bursts of activity ATP derived from creatine phosphate hydrolysis may be sufficient to support muscle work and that phosphagen would subsequently be slowly restored later.

Increased maximal activities of the key glycolytic enzymes, HK and PFK, in the brain correlate well with the known strong reliance of brain on glucose as its primary metabolic fuel, a reliance that would be further enhanced during hypoxia. The increases in these key activities may support increased glycolytic flux when oxygen-based metabolism is restricted. In contrast, a small reduction in PFK activity in the liver during hypoxia may be consistent with a primary function of this organ in glucose export during hypoxia in order to provide fermentative fuel to other organs. The primary effect of hypoxia on skeletal muscle, a strong increase in LDH activity, may be a precursor for increased glycolytic ATP production.

Information on the effects of hypoxia or anoxia on the activities of glycolytic and associated enzymes in fish tissues is quite limited. Previous studies showed that anoxia resulted in a redistribution of glycogen phosphorylase between a and b forms as well as changes in the content of fructose-2,6-bisphosphate in goldfish tissues (33). These data, taken together with kinetic constants for PFK measured in different tissues, led us to conclude that PFK represents a major control point for coordinating changes in carbohydrate metabolism in response to changes in the physiological state of animals and that the mechanism of this change includes the reversible phosphorylation of the enzyme as well as modification of the levels of the PFK allosteric activator, fructose-2,6-bisphosphate. Short-term hypoxia activated PFK in the brain and liver and HK in the brain of red mullet but had no effect on PK activity in either tissue (25). Long-term hypoxia (1.5 months) modified the activities of some glycolytic enzymes in goldfish tissues (34). Clearly, short- and long-term hypoxia or anoxia can differently affect the enzymes of carbohydrate metabolism in fish.

Hypoxia exposure significantly changed the distribution of various enzymes between free and particulate-bound states in sea scorpion tissues. Such a redistribution indicates that this mechanism may be involved in the regulation of glycolysis in these organs, providing space-time compartmentation of the pathway to serve different metabolic needs. The specific enzymes affected in different tissues could result in tissue-specific changes in flux through different pathways in a manner that serves the metabolic needs of each organ. In the brain, for example, a strong decrease in the percentage of G6PDH bound under hypoxic conditions may have a major effect on flux through the pentose phosphate shunt and on the relative consumption of glucose-6-phosphate by this pathway versus glycolysis. In the liver, the 3-fold increase in the amount of bound HK may be the key to supporting increased glucose utilization as a substrate for the hypoxic organ. A reduction in the percentage of bound PFK in skeletal muscle contrasts with an exercise-induced increase in bound PFK in trout muscle during glycolysis-fueled muscle work (14) and suggests that the hypoxic muscle of sea scorpion may exhibit a metabolic depression that actually lowers the glycolytic rate. Factors influencing the redistribution of enzymes could include several that might be due to the effects of hypoxia on cellular metabolism including a change in intracellular pH, changes in the levels of different metabolites, or reversible enzyme phosphorylation. As other studies have shown, free and bound enzymes can also differ in their stability (7,10,11) and changes in the percentage of bound enzyme could also affect the structural stability and/or the turnover rate of enzymes under stresses that alter physical conditions in the cytosol. In addition to different kinetic properties of free and bound enzymes (35), their redistribution may be an effective mechanism allowing animals to adapt to environmental changes.

The nature of the structural elements to which enzymes may bind can also be discussed. For example, in the brain, glycolytic enzymes may be associated with mitochondria or the cytoskeleton proteins, actin and tubulin (10,15), whereas in the liver association with the endoplasmic reticulum has been documented (8). In muscle, PFK interacts with actin (1-5) (as do other glycolytic enzymes) and binding is altered by the phosphorylation state of the enzyme (3). In muscle, LDH is also found to be associated with the membrane of the sarcoplasmic reticulum (10,13). Because various structural elements may be involved as subcellular sites for enzyme binding, this provides yet another mechanism for the differential control of enzymes and the pathways to which they belong. The possibility of formation of supramolecular complexes also cannot be ignored in this discussion. Although the interaction of some enzymes with structural elements reduced their activity, their association in a complex might result in a net increase in pathway flux through a multienzyme complex as a consequence of an increase in the local concentration of metabolites (10,14,15,19,35).

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The authors thank J.M. Storey for critical commentary on the manuscript and L.M. Rudenko for skillful technical assistance.

Address for correspondence: V.I. Lushchak, Department of Medical Biology and Genetics, Ivano-Frankivsk State Medical Academy, Ivano-Frankivsk, Ukraine 284000.

Received March 12, 1997. Accepted May 15, 1998.

Correspondence and Footnotes

Publication Dates

• Publication in this collection
  21 Sept 1998
• Date of issue
  Aug 1998

History

• Accepted
  15 May 1998
• Received
  12 Mar 1997